10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

More documents

Recommendations

Info

EFFECT OF AGRONOMICAL FACTORS ON THEIMPORTANCE OF VERTICILLIUM WILT OF OLIVE IN THEGUADALQUIVIR VALLEY IN ANDALUCÍA (SOUTHERNSPAIN)LÓPEZ-ESCUDERO, F.J. 1 (ag2loesj@uco.es), ROCA, J.M. 1 (ag3roloj@uco.es),MERCADO-BLANCO, J. 2 (jesus.mercado@ias.csic.es), VALVERDE-CORREDOR,A. 2 (antonio.valverde@ias.csic.es) AND BLANCO-LÓPEZ, M.A 1 (ag1bllom@uco.es).1 Dep. de Agronomía, Universidad de Córdoba, Campus Univ. Rabanales, Edificio Celestino Mutis,14071, Córdoba, Spain ;2 Dep. de Protección de Cultivos, Instituto de Agricultura Sostenible, CSIC, Alameda del Obispo s/nApdo. 14080, Córdoba, SpainVerticillium wilt of olive (VWO) is nowadays the most destructive olivedisease in the Guadalquivir Valley in Andalucía, the largest olive cultivation area inthe world, where 1.5 million ha of olive orchards are concentrated. Disease surveyswere conducted throughout the valley with the aim to update the importance anddistribution of VWO, and to assess the influence of geographical, edaphical andagronomical factors in the current spread of the disease. Disease incidence (DI) wasrecorded in 90 olive orchards affected by VWO (27, 33 and 30, in Jaén, Córdoba andSeville provinces, respectively) in a one-hundred-tree rectangular plot, chosenarbitrarily inside plantations. VWO was widely distributed in the Guadalquivir Valley,with a mean DI reaching the 20.4% of 9000 inspected trees, but with significantdifferences between provinces (25.7, 23.7 and 12%, for Jaén, Córdoba and Seville,respectively). DI was significantly higher in irrigated (20.7%) than in dry-farming(18.3%) olive orchards, being differences particularly important in the central and theupper Valley. Also, non-tilled orchards showed higher DI (25.6%) than those regularlytilled (16.3%). DI was lower when the number of trees per ha in orchards was higherthan 200. Moreover percentage of wilted plants was higher (21.5%) with theproximity of neighboring V. dahliae host crops to olive orchards, than if nonsusceptiblehosts surrounded them (11.9%). Finally, differences of DI were morestressed in plots where less than 25-year-old trees were grown and in those plantationscloser to the Guadalquivir River (less than 10 km). ‘Picual’ was identified as the mostsusceptible cultivar to the disease, reaching 41.9% of DI in the plots in which thiscultivar was identified. PCR-based molecular pathotyping of V. dahliae isolatesrecovered from wilted olive trees showed that defoliating (D) highly virulent isolateswere presented at surveyed plantations in percentages significantly higher (67.7%)than non-defoliating isolates (32.3%), specially in lower (Seville province) and upper(Jaén province) valley. This fact could explain the important increasing of incidenceand severity of VWO observed in the valley during the last decade.96
VERTICILLIUM INTERSPECIFIC HYBRIDS MIGHT NOT BE SORARE IN NATUREMELANIA COLLADO-ROMERO 1 , RAFAEL M. JIMÉNEZ-DÍAZ 1,2 ANDJESÚS MERCADO-BLANCO 11 Instituto de Agricultura Sostenible (CSIC), Apartado 4084, 14080 Córdoba, Spain(jesus.mercado@ias.csic.es);2 ETSIAM, Universidad de Córdoba, Edificio C-4, Campus Rabanales, Ctra de Madrid, km 396, 14071Córdoba, Spain.Molecular evidences for interspecific hybrid origin of Verticillium dahliaeisolates of vegetative compatibility group 3 (VCG3) were found while analyzing thepresence of multiple sequences of genes actin (Act), β-tubulin (β-Tub), calmodulin(Cal) and histone 3 (H3), and the internal transcribed sequences (ITS-1 and ITS-2) ofthe rRNA genes. Moreover, analyses of these sequences provided further consistentevidences of the hybrid origin of V. dahliae var. longisporum (VDLSP). Thus,analysis of the two alleles found for genes Act and β-Tub in V. dahliae VCG3revealed that one allele was identical to previously characterized V. dahliaesequences, whereas the other was as unrelated as those homologues found in V.nigrescens or V. albo-atrum. However, for those genes for which unique sequenceswere identified, neither homology (Cal) nor full identity (ITS) to V. dahliae werefound. None of the VCG3 sequences unrelated to V. dahliae showed completehomology in gene databases. On the other hand, none of the Act, β-Tub and Calalleles found in VDLSP isolates were identical to their homologues in V. dahliae or V.albo-atrum. Moreover, the unique H3 sequence found was identical to that existing inV. dahliae, but ITS sequences showed closer to V. albo-atrum ITS than to V. dahliaeITS. Additionally, a V. dahliae-specific polymorphic sequence was used to shed lightabout the putative parents of VCG3 and VDLSP isolates. Phylogenetic analysis of allthese sequences suggested that the hybridization event originating some of thecurrently known VDLSP isolates might have happened between a V. dahliae ofVCG1 and/or VCG4A and a closely-related V. albo-atrum as parents. On the otherhand, phylogenetic analysis and PCR markers profiling of a V. dahliae VCG3 isolatesuggested a VCG1B isolate as putative parent. However, the other parent appearedunrelated to Verticillium spp. and could not be identify according to the sequencesavailable in gene databases. Therefore, the representative VCG3 isolate examined inthis study must be considered as an interspecific hybrid between V. dahliae and as yetunidentified parent. These results suggest an important role for parasexuality indiversity and evolution in the genus Verticillium, and that interspecific hybrids withinthis genus might no be so rare in nature.97
Page 1 and 2:
10th INTERNATIONAL VERTICILLIUMSYMP
Page 3 and 4:
10th INTERNATIONAL VERTICILLIUM SYM
Page 6:
ORAL PRESENTATIONSTAXONOMY AND GENE
Page 10 and 11:
12.00 MANOLIS MARKAKISQuantificatio
Page 12 and 13:
16.40 MALTE BEINHOFFDetection and f
Page 14 and 15:
Production and scavenging of ROS du
Page 16 and 17:
C.Lucena 1 , J.A.J.Berni 2 , M.Mont
Page 18 and 19:
15.10 MARIA DOLORES GARCÍA-PEDRAJA
Page 20 and 21:
1 Agricultural Research Organizatio
Page 22 and 23:
17.15 CLOSING REMARKS17.30ELECTION
Page 24:
VERTICILLIUM COMPARATIVE GENOMICSST
Page 27 and 28:
ANALYSES OF MATING TYPE GENES IN VE
Page 29 and 30:
DEVELOPMENT AND APPLICATION OF NEW
Page 31 and 32:
QUANTITATIVE DETECTION OF MULTIPLE
Page 33 and 34:
ANALYSIS OF VERTICILLIUM LONGISPORU
Page 35 and 36:
CHORISMATE SYNTHASE AND COLONIZATIO
Page 37 and 38:
CHANGES IN ETHYLENE PERCEPTION OF A
Page 39 and 40:
IDENTIFICATION OF THE V. DAHLIAE SE
Page 41 and 42:
MOLECULAR AND GENETIC DETERMINANTS
Page 43 and 44:
CHARACTERIZATION AND GENETICS OF DI
Page 45 and 46: THE SUCROSE NON FERMENTING PROTEIN
Page 47 and 48: QTL MAPPING OF VERTICILLIUM RESISTA
Page 49 and 50: QUANTIFICATION OF DEFOLIATING AND N
Page 51 and 52: CATALYTIC SUBUNIT OF PROTEIN KINASE
Page 53 and 54: IDENTIFICATION AND CHARACTERIZATION
Page 55 and 56: AUTOPHAGY AND RESTING STRUCTURE DEV
Page 57 and 58: WILTING DISEASE IN THE HALLERTAUER
Page 59 and 60: CHARACTERISATION OF POTENTIAL PATHO
Page 61 and 62: CHARACTERIZATION OF NEP-LIKE PROTEI
Page 63 and 64: INSIGHTS INTO THE ROLE OF THE NECRO
Page 65 and 66: VERTICILLIUM LONGISPORUM-INDUCED GE
Page 67 and 68: IN VITRO ELICITATION OF PATHOGENICI
Page 69 and 70: ON VERTICILLIUM DAHLIAE PLASTICITY
Page 71 and 72: PHYSIOLOGICAL DIFFERENCES EXPRESSED
Page 73 and 74: PRODUCTION AND SCAVENGING OF ROS DU
Page 75 and 76: VERTICILLIUM WILT OF OLIVES IN SOUT
Page 77 and 78: BIOLOGICAL CONTROL OF VERTICILLIUM
Page 79 and 80: THE INFLUENCE OF AGRONOMIC FACTORS
Page 81 and 82: HIGH RESOLUTION THERMAL REMOTE SENS
Page 83 and 84: STENOTROPHOMONAS - NEW INSIGHTS INT
Page 85 and 86: CONTROL OF VERTICILLIUM WILT OF OLI
Page 87 and 88: MICROBIAL ANTAGONISTS AND COMPOST-B
Page 89 and 90: ATTEMPTS TO CONTROL VERTICILLIUM WI
Page 91 and 92: VERTICILLIUM RESISTANCE IN MAPLEJEL
Page 93 and 94: INITIAL SURVEY OF VERTICILLIUM WILT
Page 95: DETECTION AND QUANTIFICATION OF VER
Page 99 and 100: AN OVERVIEW OF VEGETATIVE COMPATIBI
Page 101 and 102: DETERMINATION OF PATHOTYPES OF VERT
Page 103 and 104: POTENTIAL OF LIGNIN INCORPORATION I
Page 105 and 106: A KNOWLEDGE-BASED SYSTEM TO PREDICT
Page 107 and 108: EFFECTS OF ACIBENZOLAR-S-METHYL ON
Page 109 and 110: MOLECULAR ANALYSIS OF ENDOPHYTIC CO
Page 111 and 112: AN OUTBREAK OF VERTICILLIUM WILT IN
Page 113 and 114: EFFECT OF GUANITO ON OLIVE PLANTLET
Page 115 and 116: Grisebachstr. 8D-37077 GOETTINGEN,
Page 117 and 118: DR. EVA HÄFFNERFreie Universität
Page 119 and 120: The Pennsylvania State UniversityUN
Page 121 and 122: Department of Plant Pathology75 Ier
Page 123 and 124: FAX: +31-317-483412E-mail: Partha.s
Page 125 and 126: DR. DIMITRIOS TSITSIYIANNISAgricult
Page 127 and 128: AUTHORS’ INDEXAADAM..............
Page 129 and 130: KKAMBLE ...........................
Page 131: TRAPERO-CASAS .....................
show all

10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

Create successful ePaper yourself

Delete template?

Save as template?