10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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FUNGAL ADHESION TO PLANTS – THE FIRST STEP OFEARLY INFECTION AND SYSTEMIC COLONIZATION OFBRASSICA NAPUS BY <strong>VERTICILLIUM</strong> LONGISPORUM.TUAN VAN TRAN, SUSANNA A. BRAUS-STROMEYER AND GERHARD H.BRAUSInstitute of Microbiology and Genetics, Goettingen, GermanyE-mail: Tuan.Tran-van@biologie.uni-goettingen.deVerticillium longisporum is a major pathogen for oilseed rape (Brassicanapus). This fungus induces early senescence and causes severe economic losses. V.longisporum infects host-plants via the roots and lives biotrophically in the xylemvessels of B. napus. Currently no fungicides are available to cure infected plants.Adhesion is a crucial step for the early infection and colonization of many pathogenicfungi. Proteins required for adhesion of plant fungal pathogens play important rolesduring pre-penetration, growth, biofilm formation as well as pathogenicity.The goal of this project is to investigate adhesive proteins that may supportsuccessful infection and xylem vessel colonization of V. longisporum. Adhesioncomplementation assays in non-adherent yeasts Saccharomyces cerevisiae andbioinformatics analyses of the fungal cDNA library revealed ten genes encodingputatively secreted proteins that may be involved in adhesion of the fungus. We couldshow that eight of these putatively secreted proteins were up-regulated when thefungus interacted with roots of the host plant. In addition, two transcriptionalregulators from V. longisporum induce strong adhesion of S. cerevisae to the agarsurface. These transcription factors belong to the zinc finger family and were reportedto regulate many essential genes in filamentous fungi such as genes for toxinproduction, for conidial formation or for cell wall biosynthesis, etc. We are currentlyanalyzing the knockdowns of the corresponding genes in V. longisporum.102
POTENTIAL OF LIGNIN INCORPORATION IN SOIL TOCONTROL <strong>VERTICILLIUM</strong> WILT OF CAULIFLOWERFRANÇA S.C. 1 , VAN BENEDEN, S. 1 , SPIESSENS, K. 2 , DE ROOSTER, L. 2 ,POLLET, S. 3 , CALLENS, D. 3 AND HÖFTE, M. 11 Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering,Ghent University, Coupure links 653, 9000 Ghent, Belgium;.2 Proefstation voor de Groenteteelt, Sint-Katelijne-Waver, Belgium.;3 Provinciaal Onderzoeks- en Voorlichtingscentrum voor Land- en Tuinbouw Rumbeke-Beitem,Belgium.E-mail: Soraya.deCarvalhoFranca@UGent.beVerticillium wilt is an important problem in Belgian cauliflower production.The disease is caused by Verticillium longisporum, a soil-borne pathogen which canpersist for many years in soil in the form of microsclerotia. Organic amendments,such as certain crop residues and side products have been used to control Verticilliumwilt of other crops. Previous work in our lab has shown that incorporation of Kraftpine lignin, a side product of the paper industry, decreased the viability of V.longisporum microsclerotia in soil. In addition, lignin combined with introducedPseudomonas spp. in soil was more effective than each treatment alone. These resultsraised the hypothesis that lignin incorporation in soil weakens the microsclerotia andmakes them more susceptible for antagonists. Further investigation was carried out totest the effect of lignin on Verticillium wilt of cauliflower and explore themechanisms underlying the disease control. Lower disease incidence and severitywere observed on cauliflower plants grown in pots containing naturally infested soilamended with lignin (1% w/w) compared to the control plants, confirming thepotential of lignin incorporation for managing the disease. However, the effect oflignin on microsclerotia seems to be soil dependent. When lignin was incorporatedinto two different soil types – Leest and Oppuurs, the number of viable Verticilliummicrosclerotia was reduced in Leest soil, but not in Oppuurs soil. Changes inmicrobial populations in these soils were studied using phospholipid fatty acidanalysis. It revealed a similar increase in Gram negative bacteria upon ligninamendment in both soils and a higher increase in fungi and acitnomycetes in Leestsoil than in Oppuurs soil. We suggest that the differential stimulation of fungi andactinomycetes that occurred in Leest soil was probably necessary for the successfulresult obtained in this soil type, but the decrease of microsclerotia viability might bethe outcome of a complex microbial interaction involving Gram negative bacteria aswell.103
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10th INTERNATIONAL VERTICILLIUMSYMP
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10th INTERNATIONAL VERTICILLIUM SYM
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ORAL PRESENTATIONSTAXONOMY AND GENE
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12.00 MANOLIS MARKAKISQuantificatio
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16.40 MALTE BEINHOFFDetection and f
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Production and scavenging of ROS du
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C.Lucena 1 , J.A.J.Berni 2 , M.Mont
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15.10 MARIA DOLORES GARCÍA-PEDRAJA
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1 Agricultural Research Organizatio
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17.15 CLOSING REMARKS17.30ELECTION
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VERTICILLIUM COMPARATIVE GENOMICSST
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ANALYSES OF MATING TYPE GENES IN VE
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DEVELOPMENT AND APPLICATION OF NEW
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QUANTITATIVE DETECTION OF MULTIPLE
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ANALYSIS OF VERTICILLIUM LONGISPORU
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CHORISMATE SYNTHASE AND COLONIZATIO
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CHANGES IN ETHYLENE PERCEPTION OF A
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IDENTIFICATION OF THE V. DAHLIAE SE
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MOLECULAR AND GENETIC DETERMINANTS
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CHARACTERIZATION AND GENETICS OF DI
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THE SUCROSE NON FERMENTING PROTEIN
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QTL MAPPING OF VERTICILLIUM RESISTA
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QUANTIFICATION OF DEFOLIATING AND N
Page 51 and 52: CATALYTIC SUBUNIT OF PROTEIN KINASE
Page 53 and 54: IDENTIFICATION AND CHARACTERIZATION
Page 55 and 56: AUTOPHAGY AND RESTING STRUCTURE DEV
Page 57 and 58: WILTING DISEASE IN THE HALLERTAUER
Page 59 and 60: CHARACTERISATION OF POTENTIAL PATHO
Page 61 and 62: CHARACTERIZATION OF NEP-LIKE PROTEI
Page 63 and 64: INSIGHTS INTO THE ROLE OF THE NECRO
Page 65 and 66: VERTICILLIUM LONGISPORUM-INDUCED GE
Page 67 and 68: IN VITRO ELICITATION OF PATHOGENICI
Page 69 and 70: ON VERTICILLIUM DAHLIAE PLASTICITY
Page 71 and 72: PHYSIOLOGICAL DIFFERENCES EXPRESSED
Page 73 and 74: PRODUCTION AND SCAVENGING OF ROS DU
Page 75 and 76: VERTICILLIUM WILT OF OLIVES IN SOUT
Page 77 and 78: BIOLOGICAL CONTROL OF VERTICILLIUM
Page 79 and 80: THE INFLUENCE OF AGRONOMIC FACTORS
Page 81 and 82: HIGH RESOLUTION THERMAL REMOTE SENS
Page 83 and 84: STENOTROPHOMONAS - NEW INSIGHTS INT
Page 85 and 86: CONTROL OF VERTICILLIUM WILT OF OLI
Page 87 and 88: MICROBIAL ANTAGONISTS AND COMPOST-B
Page 89 and 90: ATTEMPTS TO CONTROL VERTICILLIUM WI
Page 91 and 92: VERTICILLIUM RESISTANCE IN MAPLEJEL
Page 93 and 94: INITIAL SURVEY OF VERTICILLIUM WILT
Page 95 and 96: DETECTION AND QUANTIFICATION OF VER
Page 97 and 98: VERTICILLIUM INTERSPECIFIC HYBRIDS
Page 99 and 100: AN OVERVIEW OF VEGETATIVE COMPATIBI
Page 101: DETERMINATION OF PATHOTYPES OF VERT
Page 105 and 106: A KNOWLEDGE-BASED SYSTEM TO PREDICT
Page 107 and 108: EFFECTS OF ACIBENZOLAR-S-METHYL ON
Page 109 and 110: MOLECULAR ANALYSIS OF ENDOPHYTIC CO
Page 111 and 112: AN OUTBREAK OF VERTICILLIUM WILT IN
Page 113 and 114: EFFECT OF GUANITO ON OLIVE PLANTLET
Page 115 and 116: Grisebachstr. 8D-37077 GOETTINGEN,
Page 117 and 118: DR. EVA HÄFFNERFreie Universität
Page 119 and 120: The Pennsylvania State UniversityUN
Page 121 and 122: Department of Plant Pathology75 Ier
Page 123 and 124: FAX: +31-317-483412E-mail: Partha.s
Page 125 and 126: DR. DIMITRIOS TSITSIYIANNISAgricult
Page 127 and 128: AUTHORS’ INDEXAADAM..............
Page 129 and 130: KKAMBLE ...........................
Page 131: TRAPERO-CASAS .....................
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10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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