10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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THE MOLECULAR GENETICS OF THE INTERACTION OFVERTICILLIUM WITH THE PLANT HOSTS ARABIDOPSIS ANDTOMATOBART P.H.J. THOMMA, KOSTE A. YADETA, SAJID REHMAN, URSULAELLENDORFF, RONNIE DE JONGE, H. PETER VAN ESSE, ANJAKOMBRINK, GRARDY C.M. VAN DEN BERG, ZHAO ZHANG, ERMISYANES PAZ, PARTHASARATHY SANTHANAM AND EMILIE FRADINLaboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen,The NetherlandsSo far, a locus responsible for resistance against Verticillium has been cloned onlyfrom tomato [1]. This Ve locus governs resistance against race 1 strains of V. dahliae andV. albo-atrum, and contains two closely linked inversely oriented genes, Ve1 and Ve2.We recently investigated the role of Ve1 and Ve2 in Verticillium resistance of tomato.Surprisingly, we found that Ve1, but not Ve2, mediates Verticillium resistance in tomato[2]. Using VIGS, we determined the role of a number of known disease resistancesignaling components in Verticillium resistance. We found that the receptor-like kinaseSERK3 (BAK1) is required for Ve1-mediated resistance, providing the first example ofrace-specific disease resistance for which SERK3/BAK1 is required [2]. Through cDNA-AFLP followed by VIGS analysis of candidate genes we are trying to identify novelcomponents that are required for Ve1 mediated-resistance in tomato.In addition to tomato, we also use Arabidopsis as a host for Verticillium dahliae.We recently discovered a role for gene silencing in Verticillium defense [3]. Furthermore,mutants displaying enhanced Verticillium resistance have been isolated and characterized.Finally, we are interested to characterize the secretome of V. dahliae, potentialvirulence factors that are secreted in planta, amounting to over 780 proteins. Thesecretome contains seven lysin motif (LysM) effectors that are homologous to therecently identified C. fulvum effector protein Ecp6 [4,5]. The role of LysM effectors inpathogen virulence will be addressed.References:[1] Kawchuk L et al. 2001. Tomato Ve disease resistance genes encode cell surface-like receptors. Proc. Natl.Acad. Sci. USA 98:6511-6515. [2] Fradin EF et al. 2009. Genetic dissection of Verticillium wilt resistancemediated 1 by tomato Ve1. Plant Physiol. 150:320-332. [3] Ellendorff U et al.2009. RNA silencing is required forArabidopsis defense against Verticillium wilt disease. J. Exp. Bot. 60:591-602. [4] Bolton MD et al. 2008. Thenovel Cladosporium fulvum lysin motif effector Ecp6 is a virulence factor with orthologs in other fungal species.Mol. Microbiol. 69:119-136. [5] de Jonge R and Thomma BPHJ 2009. Fungal LysM effectors - extinguishers ofhost immunity? Trends Microbiol. 17:151-157.26
ANALYSES OF MATING TYPE GENES IN VERTICILLIUMSPECIESDEZ BARBARA 8* , PATRIK INDERBITZIN 2, EMILY CLEWES 8 , CLAREGRANT 8 , STEVE KLOSTERMAN 1 , KRISHNA V. SUBBARAO 2 ,KATHERINE F. DOBINSON 3 , PAOLA VERONESE 4 , BART P. H. J.THOMMA 5 , MARIA D. GARCIA-PEDRAJAS 6 , AMY ANCHIETA 1 , ZEHUACHEN 7 , RONNIE DE JONGE 5 , PARTHA SANTHANAM 5 , KARUNAKARANMARUTHACHALAM 2 , ZAHI ATALLAH 2 , STEFAN AMYOTTE 3 , ZAHIPAZ 9 , DAVID I. HEIMAN 7 , SARAH YOUNG 7 , QIANDONG ZENG 7 ,REINHARD ENGELS 7 , MICHAEL KOEHRSEN 7 , JAMES GALAGAN 7 ,BRUCE BIRREN 7 , CHRISTINA CUOMO 7 , SEOGCHAN KANG 10 , SCOTT E.GOLD 9 AND LI-JUN MA 7*Dez.Barbara@warwick.ac.uk1 USDA-ARS, Salinas, CA, USA; 2 University of California Davis, Davis, CA, USA; 3 Agriculture andAgri-Food Canada, London, Ontario, Canada, 4 North Carolina State University, Raleigh, NC, USA; 5Wageningen University and Research Centre, Wageningen, The Netherlands; 6 Estacion ExperimentalLa Mayora, CSIC, Malaga, Spain; 7 The Broad Institute, Cambridge, MA, USA; 8 University ofWarwick, Wellesbourne, Warwick, UK 9 University of Georgia, Athens, GA, USA; 10 Pennsylvania StateUniversity, University Park, PA, USASexual mating has not been recorded in any of the plant pathogenic Verticilliumspecies but as ascomycetes they are presumably derived from species which wereonce sexual. Two idiomorphic genes with DNA binding motifs determine mating typein many fungi. Such genes determining mating type have been reported in otherasexual fungi and we have looked for them in Verticillium.A MAT1-2-1 gene (with an HMG-box motif) has been reported from Japaneseand other isolates of V. dahliae (Usami et al, 2009, Phys. Mol. Pl Path. 73, 133) butthis group have not reported finding a MAT1-1-1 gene (which should carry an alphaboxmotif). Building both on the work of Usami et al. and the sequence coming fromthe Verticillium genome sequencing project we here report the presence of bothmating type idiomorphs in isolates of V. dahliae. We have also found a MAT1-2-1gene homologue in Non-Lucerne isolates of V. albo-atrum whilst Lucerne isolates ofV. albo-atrum carry the MAT1-1-1 idiomorph. Within the two idiomorphs there aresequence variants and preliminary data suggests this variation may correlate withother properties, probably as an indicator of clonal lineages rather than causallyrelated.Long-spored isolates from cruciferous hosts (V. longisporum) are interspecificamphihaploid hybrids and as expected these carry two distinct copies of the matingtype locus. However these have sequences distinct from either of the haploid species.The significance of this in relation to the origins of these isolates will be discussed.27
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Page 6: ORAL PRESENTATIONSTAXONOMY AND GENE
Page 10 and 11: 12.00 MANOLIS MARKAKISQuantificatio
Page 12 and 13: 16.40 MALTE BEINHOFFDetection and f
Page 14 and 15: Production and scavenging of ROS du
Page 16 and 17: C.Lucena 1 , J.A.J.Berni 2 , M.Mont
Page 18 and 19: 15.10 MARIA DOLORES GARCÍA-PEDRAJA
Page 20 and 21: 1 Agricultural Research Organizatio
Page 22 and 23: 17.15 CLOSING REMARKS17.30ELECTION
Page 24: VERTICILLIUM COMPARATIVE GENOMICSST
Page 29 and 30: DEVELOPMENT AND APPLICATION OF NEW
Page 31 and 32: QUANTITATIVE DETECTION OF MULTIPLE
Page 33 and 34: ANALYSIS OF VERTICILLIUM LONGISPORU
Page 35 and 36: CHORISMATE SYNTHASE AND COLONIZATIO
Page 37 and 38: CHANGES IN ETHYLENE PERCEPTION OF A
Page 39 and 40: IDENTIFICATION OF THE V. DAHLIAE SE
Page 41 and 42: MOLECULAR AND GENETIC DETERMINANTS
Page 43 and 44: CHARACTERIZATION AND GENETICS OF DI
Page 45 and 46: THE SUCROSE NON FERMENTING PROTEIN
Page 47 and 48: QTL MAPPING OF VERTICILLIUM RESISTA
Page 49 and 50: QUANTIFICATION OF DEFOLIATING AND N
Page 51 and 52: CATALYTIC SUBUNIT OF PROTEIN KINASE
Page 53 and 54: IDENTIFICATION AND CHARACTERIZATION
Page 55 and 56: AUTOPHAGY AND RESTING STRUCTURE DEV
Page 57 and 58: WILTING DISEASE IN THE HALLERTAUER
Page 59 and 60: CHARACTERISATION OF POTENTIAL PATHO
Page 61 and 62: CHARACTERIZATION OF NEP-LIKE PROTEI
Page 63 and 64: INSIGHTS INTO THE ROLE OF THE NECRO
Page 65 and 66: VERTICILLIUM LONGISPORUM-INDUCED GE
Page 67 and 68: IN VITRO ELICITATION OF PATHOGENICI
Page 69 and 70: ON VERTICILLIUM DAHLIAE PLASTICITY
Page 71 and 72: PHYSIOLOGICAL DIFFERENCES EXPRESSED
Page 73 and 74: PRODUCTION AND SCAVENGING OF ROS DU
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VERTICILLIUM WILT OF OLIVES IN SOUT
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BIOLOGICAL CONTROL OF VERTICILLIUM
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THE INFLUENCE OF AGRONOMIC FACTORS
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HIGH RESOLUTION THERMAL REMOTE SENS
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STENOTROPHOMONAS - NEW INSIGHTS INT
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CONTROL OF VERTICILLIUM WILT OF OLI
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MICROBIAL ANTAGONISTS AND COMPOST-B
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ATTEMPTS TO CONTROL VERTICILLIUM WI
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VERTICILLIUM RESISTANCE IN MAPLEJEL
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INITIAL SURVEY OF VERTICILLIUM WILT
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DETECTION AND QUANTIFICATION OF VER
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VERTICILLIUM INTERSPECIFIC HYBRIDS
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AN OVERVIEW OF VEGETATIVE COMPATIBI
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DETERMINATION OF PATHOTYPES OF VERT
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POTENTIAL OF LIGNIN INCORPORATION I
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A KNOWLEDGE-BASED SYSTEM TO PREDICT
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EFFECTS OF ACIBENZOLAR-S-METHYL ON
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MOLECULAR ANALYSIS OF ENDOPHYTIC CO
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AN OUTBREAK OF VERTICILLIUM WILT IN
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EFFECT OF GUANITO ON OLIVE PLANTLET
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Grisebachstr. 8D-37077 GOETTINGEN,
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DR. EVA HÄFFNERFreie Universität
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The Pennsylvania State UniversityUN
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Department of Plant Pathology75 Ier
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FAX: +31-317-483412E-mail: Partha.s
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DR. DIMITRIOS TSITSIYIANNISAgricult
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AUTHORS’ INDEXAADAM..............
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KKAMBLE ...........................
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TRAPERO-CASAS .....................
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10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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