10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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PROTEOMIC ANALYSES OF DEFENCE GENE EXPRESSION INA MODEL TOMATO-<strong>VERTICILLIUM</strong> PATHOSYSTEMJANE ROBB 1 , BARBARA LEE 1 , ALEX KUROSKY 2 AND ROSS N. NAZAR 11 Molecular and Cellular Biology, University of Guelph, ON, Canada and Department of Biochemistryand2 Molecular Biology, University of Texas Medical Branch, Galveston, TX, USARelatively little is known about the molecular mechanisms utilized by plantsto defend themselves against fungal vascular pathogens. Based on DNA microarrayanalyses, in past studies we used a model tomato-Verticillium pathosystem to comparethe global expression of genes in compatible and incompatible interactions. Whilevery significant patterns of mRNA changes were defined, how these are translated atthe protein level remained unclear. In the present study, 2D gel electrophoresis andproteomic analyses were applied to evaluate the actual changes in protein levels.Whole cell protein was extracted simultaneously with RNA to permit parallelcomparisons of both mRNA and proteins from the same samples. Proteinsrepresenting 13 of the most intense changes were identified using mass spectrometry.Three were products of commonly identified pathogenesis related genes, three wereperoxidase related and two were osmotin related proteins, all products of genescommonly reported to be involved in genetic responses to stress or pathogens. Thelevels of induction relative to other cellular proteins were particularly striking, anobservation which underlines the plant's heroic systemic response to a vasculardisease such as Verticillium wilt.Supported by the Natural Sciences and Engineering Research Council of Canada.32
ANALYSIS OF <strong>VERTICILLIUM</strong> LONGISPORUM-INDUCEDGENE EXPRESSION IN ARABIDOPSIS THALIANACHRİSTİANE GATZ, ELKE GERHARD BRAUS, ELKE DİEDERİCHSEN,WOLFGANG DRÖGE-LASER, VOLKER LİPKA, IVO FEUßNER, PETRKARLOVSKY, ANDREA POLLE, THOMAS TEİCHMANN AND ANDREASVON TİEDEMANNGeorg-August-University Goettingen, GermanyThe Verticillium research unit, which consists of eight partners of the GeorgAugust University Göttingen and one partner of the Free University Berlin, haschosen to elucidate the interaction between V. longisporum and Arabidopsis thalianaand Brassica napus as a common experimental system to address the question howplants can sense and react to foreign organisms in the xylem. One fruitful aspect ofthis research group is the strong focus on one experimental system that is investigatedusing the different expertises of laboratories working in the fields of biochemistry,cell biology, molecular and applied genetics, plant physiology, plant pathology, andmicrobiology, and entomology. The project profits from the modern infrastructure(proteomics, transcriptomics, metabolomics, microscopy) available on campus. Indetail, we address the question concerning the identity of the fungal PAMPs orelicitors that evoke plant responses to Verticillium longisporum in roots and in thexylem. Second, we analyze, which responses of the plant serve to accommodate thefungus and which responses serve defense mechanisms (see Abstracts byDiederichsen et al., Häffner et al., Reusche et al.). Third, we aim to elucidate theplant signal transduction chains that elicit the responses (see Abstracts by Ralhan etal., Teichmann et al., Weiberg et al.). Finally, the adaptation of the fungus to living inthe xylem is explored (see Abstracts by Braus-Stromeier et al. and van Tran et al.).The talk will give a brief overview of the research approaches of the different groups.Finally, I will present data from our group that focus on the use of Verticilliumlongisporum-induced marker genes to elucidate the contribution of known andunknown singaling pathways to the gene expression pattern elicited by the plant(See also Abstract by Ralhan et al.).33
Page 1 and 2: 10th INTERNATIONAL VERTICILLIUMSYMP
Page 3 and 4: 10th INTERNATIONAL VERTICILLIUM SYM
Page 6: ORAL PRESENTATIONSTAXONOMY AND GENE
Page 10 and 11: 12.00 MANOLIS MARKAKISQuantificatio
Page 12 and 13: 16.40 MALTE BEINHOFFDetection and f
Page 14 and 15: Production and scavenging of ROS du
Page 16 and 17: C.Lucena 1 , J.A.J.Berni 2 , M.Mont
Page 18 and 19: 15.10 MARIA DOLORES GARCÍA-PEDRAJA
Page 20 and 21: 1 Agricultural Research Organizatio
Page 22 and 23: 17.15 CLOSING REMARKS17.30ELECTION
Page 24: VERTICILLIUM COMPARATIVE GENOMICSST
Page 27 and 28: ANALYSES OF MATING TYPE GENES IN VE
Page 29 and 30: DEVELOPMENT AND APPLICATION OF NEW
Page 31: QUANTITATIVE DETECTION OF MULTIPLE
Page 35 and 36: CHORISMATE SYNTHASE AND COLONIZATIO
Page 37 and 38: CHANGES IN ETHYLENE PERCEPTION OF A
Page 39 and 40: IDENTIFICATION OF THE V. DAHLIAE SE
Page 41 and 42: MOLECULAR AND GENETIC DETERMINANTS
Page 43 and 44: CHARACTERIZATION AND GENETICS OF DI
Page 45 and 46: THE SUCROSE NON FERMENTING PROTEIN
Page 47 and 48: QTL MAPPING OF VERTICILLIUM RESISTA
Page 49 and 50: QUANTIFICATION OF DEFOLIATING AND N
Page 51 and 52: CATALYTIC SUBUNIT OF PROTEIN KINASE
Page 53 and 54: IDENTIFICATION AND CHARACTERIZATION
Page 55 and 56: AUTOPHAGY AND RESTING STRUCTURE DEV
Page 57 and 58: WILTING DISEASE IN THE HALLERTAUER
Page 59 and 60: CHARACTERISATION OF POTENTIAL PATHO
Page 61 and 62: CHARACTERIZATION OF NEP-LIKE PROTEI
Page 63 and 64: INSIGHTS INTO THE ROLE OF THE NECRO
Page 65 and 66: VERTICILLIUM LONGISPORUM-INDUCED GE
Page 67 and 68: IN VITRO ELICITATION OF PATHOGENICI
Page 69 and 70: ON VERTICILLIUM DAHLIAE PLASTICITY
Page 71 and 72: PHYSIOLOGICAL DIFFERENCES EXPRESSED
Page 73 and 74: PRODUCTION AND SCAVENGING OF ROS DU
Page 75 and 76: VERTICILLIUM WILT OF OLIVES IN SOUT
Page 77 and 78: BIOLOGICAL CONTROL OF VERTICILLIUM
Page 79 and 80: THE INFLUENCE OF AGRONOMIC FACTORS
Page 81 and 82: HIGH RESOLUTION THERMAL REMOTE SENS
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STENOTROPHOMONAS - NEW INSIGHTS INT
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CONTROL OF VERTICILLIUM WILT OF OLI
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MICROBIAL ANTAGONISTS AND COMPOST-B
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ATTEMPTS TO CONTROL VERTICILLIUM WI
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VERTICILLIUM RESISTANCE IN MAPLEJEL
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INITIAL SURVEY OF VERTICILLIUM WILT
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DETECTION AND QUANTIFICATION OF VER
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VERTICILLIUM INTERSPECIFIC HYBRIDS
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AN OVERVIEW OF VEGETATIVE COMPATIBI
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DETERMINATION OF PATHOTYPES OF VERT
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POTENTIAL OF LIGNIN INCORPORATION I
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A KNOWLEDGE-BASED SYSTEM TO PREDICT
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EFFECTS OF ACIBENZOLAR-S-METHYL ON
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MOLECULAR ANALYSIS OF ENDOPHYTIC CO
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AN OUTBREAK OF VERTICILLIUM WILT IN
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EFFECT OF GUANITO ON OLIVE PLANTLET
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Grisebachstr. 8D-37077 GOETTINGEN,
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DR. EVA HÄFFNERFreie Universität
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The Pennsylvania State UniversityUN
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Department of Plant Pathology75 Ier
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FAX: +31-317-483412E-mail: Partha.s
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DR. DIMITRIOS TSITSIYIANNISAgricult
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AUTHORS’ INDEXAADAM..............
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KKAMBLE ...........................
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TRAPERO-CASAS .....................
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