10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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MOLECULAR AND BIOCHEMICAL ANALYSES OF DEFENSESAND COUNTER-DEFENSES IN THE POTATO X VERTICILLIUMDAHLIAE PATHOSYSTEMABDELBASSET EL HADRAMI, LORNE R. ADAM AND FOUAD DAAYFUniversity of Manitoba, Department of Plant Science, 222 Agriculture Building, Winnipeg, Manitoba,R3T 2N2, Canada. elhadrami@cc.umanitoba.caIn our quest to provide alternative strategies for potato Verticilium wiltmanagement, we pre-screened a set of bacteria and plant extracts for their efficacy inreducing disease severity under controlled and field conditions. In this preliminaryscreening, the most effective treatments acted through enhancement of plant defenseresponses. Under controlled conditions, the changes in the host response to V.dahliae, in presence or in absence of these treatments, included a variation in solublephenolics. In particular, the flavonol glycoside rutin (querectin 3-O-rutinoside) washighly induced in response to the most effective treatments (up to 160 μM). Theselevels were revealed to be toxic to V. dahliae in vitro. Treatments that wereineffective still induced the same compound, but only to levels below the toxicthreshold. Additional investigations led us to determine the ability of V. dahliae tocleave and use the sugar moiety of rutin (rutinoside: glycosyl-rhamnoside) as a carbonsource and to further metabolize the remaining flavonol (quercetin) to by-productssuch as phloroglucinol and protocatechoylphloroglucinol carboxylic acid (2-PCPGCA). This ability allows V. dahliae overcome the toxicity of some of potatodefense-related metabolites. Furthermore, it allows it to generate phloroglucinolderivatives that could enhance its prevalence in the rhizosphere. The 2-PCPGCA isalso a source of protocatechoyl acid, one of the precursors for salicylic acid (SA),which could trigger SA-pathway in planta, thus interfering with the jasmonic acid(JA)-pathway, seemingly the main signaling pathway induced in potato against V.dahliae. This study illustrates a defense-counter defense race between potato and V.dahliae. It also shows that any biocontrol agent able to help the plant winning thisrace would be beneficial in controlling Verticillium wilt.36
CHANGES IN ETHYLENE PERCEPTION OF ARABIDOPSISPLANTS LEAD TO DIFFERENTIAL DEFENCE RESPONSESAGAINST VERTICILLIUM DAHLIAEI.S. PANTELIDES, S. E. TJAMOS, AND E. J. PAPLOMATASDepartment of Plant Pathology, Agricultural University of Athens, 75 Iera Odos, 118 55 Athens,GreeceThe defence components of Arabidopsis thaliana plants against Verticilliumdahliae were explored by the responses of mutant plants impaired in known pathogenresponse pathways. Pathogenicity tests of mutant plants revealed enhanced resistancein etr1-1 [ethylene (ET) receptor mutant] plants, but not in salicylic acid-, jasmonicacid or other ET-deficient mutants, indicating a crucial role of ETR1 in defenceagainst V. dahliae. Quantitative Real-time PCR analysis revealed that the decrease insymptom severity shown in etr1-1 plants was associated with significant reductions inthe growth of the pathogen in the vascular tissues of the plants, suggesting thatimpaired perception of ET via ETR1 results in increased disease resistance.Furthermore, the extent of V. dahliae growth within the vascular tissues of plants wasdetermined to be positively correlated with disease severity. Microarrays and RealtimePCR analysis of the expression levels of defence related genes, revealeddifferential transcriptional changes of the etr1-1 compared to wild-type and ein4 (ETreceptor mutant) plants, in response to V. dahliae infection. The activation andincreased accumulation of the PR-1, PR-2, PR-5, GSTF12, GSTU16, CHI-1, CHI-2and Myb75 genes, observed in etr1-1 plants after V. dahliae inoculation, indicate thatthe defence response of etr1-1 plants is dependent on a set of defence genes activatedupon pathogen attack.37
Page 1 and 2: 10th INTERNATIONAL VERTICILLIUMSYMP
Page 3 and 4: 10th INTERNATIONAL VERTICILLIUM SYM
Page 6: ORAL PRESENTATIONSTAXONOMY AND GENE
Page 10 and 11: 12.00 MANOLIS MARKAKISQuantificatio
Page 12 and 13: 16.40 MALTE BEINHOFFDetection and f
Page 14 and 15: Production and scavenging of ROS du
Page 16 and 17: C.Lucena 1 , J.A.J.Berni 2 , M.Mont
Page 18 and 19: 15.10 MARIA DOLORES GARCÍA-PEDRAJA
Page 20 and 21: 1 Agricultural Research Organizatio
Page 22 and 23: 17.15 CLOSING REMARKS17.30ELECTION
Page 24: VERTICILLIUM COMPARATIVE GENOMICSST
Page 27 and 28: ANALYSES OF MATING TYPE GENES IN VE
Page 29 and 30: DEVELOPMENT AND APPLICATION OF NEW
Page 31 and 32: QUANTITATIVE DETECTION OF MULTIPLE
Page 33 and 34: ANALYSIS OF VERTICILLIUM LONGISPORU
Page 35: CHORISMATE SYNTHASE AND COLONIZATIO
Page 39 and 40: IDENTIFICATION OF THE V. DAHLIAE SE
Page 41 and 42: MOLECULAR AND GENETIC DETERMINANTS
Page 43 and 44: CHARACTERIZATION AND GENETICS OF DI
Page 45 and 46: THE SUCROSE NON FERMENTING PROTEIN
Page 47 and 48: QTL MAPPING OF VERTICILLIUM RESISTA
Page 49 and 50: QUANTIFICATION OF DEFOLIATING AND N
Page 51 and 52: CATALYTIC SUBUNIT OF PROTEIN KINASE
Page 53 and 54: IDENTIFICATION AND CHARACTERIZATION
Page 55 and 56: AUTOPHAGY AND RESTING STRUCTURE DEV
Page 57 and 58: WILTING DISEASE IN THE HALLERTAUER
Page 59 and 60: CHARACTERISATION OF POTENTIAL PATHO
Page 61 and 62: CHARACTERIZATION OF NEP-LIKE PROTEI
Page 63 and 64: INSIGHTS INTO THE ROLE OF THE NECRO
Page 65 and 66: VERTICILLIUM LONGISPORUM-INDUCED GE
Page 67 and 68: IN VITRO ELICITATION OF PATHOGENICI
Page 69 and 70: ON VERTICILLIUM DAHLIAE PLASTICITY
Page 71 and 72: PHYSIOLOGICAL DIFFERENCES EXPRESSED
Page 73 and 74: PRODUCTION AND SCAVENGING OF ROS DU
Page 75 and 76: VERTICILLIUM WILT OF OLIVES IN SOUT
Page 77 and 78: BIOLOGICAL CONTROL OF VERTICILLIUM
Page 79 and 80: THE INFLUENCE OF AGRONOMIC FACTORS
Page 81 and 82: HIGH RESOLUTION THERMAL REMOTE SENS
Page 83 and 84: STENOTROPHOMONAS - NEW INSIGHTS INT
Page 85 and 86: CONTROL OF VERTICILLIUM WILT OF OLI
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MICROBIAL ANTAGONISTS AND COMPOST-B
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ATTEMPTS TO CONTROL VERTICILLIUM WI
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VERTICILLIUM RESISTANCE IN MAPLEJEL
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INITIAL SURVEY OF VERTICILLIUM WILT
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DETECTION AND QUANTIFICATION OF VER
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VERTICILLIUM INTERSPECIFIC HYBRIDS
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AN OVERVIEW OF VEGETATIVE COMPATIBI
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DETERMINATION OF PATHOTYPES OF VERT
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POTENTIAL OF LIGNIN INCORPORATION I
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A KNOWLEDGE-BASED SYSTEM TO PREDICT
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EFFECTS OF ACIBENZOLAR-S-METHYL ON
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MOLECULAR ANALYSIS OF ENDOPHYTIC CO
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AN OUTBREAK OF VERTICILLIUM WILT IN
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EFFECT OF GUANITO ON OLIVE PLANTLET
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Grisebachstr. 8D-37077 GOETTINGEN,
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DR. EVA HÄFFNERFreie Universität
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The Pennsylvania State UniversityUN
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Department of Plant Pathology75 Ier
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FAX: +31-317-483412E-mail: Partha.s
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DR. DIMITRIOS TSITSIYIANNISAgricult
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AUTHORS’ INDEXAADAM..............
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KKAMBLE ...........................
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TRAPERO-CASAS .....................
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10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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