JofIMAB-2016-vol22-issue3

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mans and animals. WHO/CDS/EPR/ 2006.7:89. [Internet] 11. Harvey RA, Cornelissen CN, Fisher BD. Microbiology. 3rd ed. Lippincott Williams & Wilkins. c2013. p.129-149. 12. Enikova RK. Hygiene of nutrition. 1st ed. Pleven:Medical University. EA-AD. 2014; p.151-157 [in Bulgarian] 13. Enikova RK. [Microbiological processes and safety of Bulgarian white cheese]. [in Bulgarian] Bulgarian National Focal Point of EFSA. 2010; 36 p. [Internet] 14. European commission health and consumers directorate-general. Working Document on Eradication of Bovine, Sheep and Goats Brucellosis in the EU. SANCO/6095/2009. 15. Denk A, Demirdag K, Kalkan A, Ozden M, Cetinkaya B, Kilic S. In vitro activity of Brucella melitensis isolates to various antimicrobials in Turkey. Infect Dis (Lond). 2015 Jun;47(6):364- 369. [PubMed] Please cite this article as: Vasileva BI, Karcheva MD, Birdanova VA, Valkov MV, Nikolov GN, Valkov AV. Outbreak investigation and control of Brucellosis. J of IMAB. 2016 Jul-Sep;22(3):1253-1255. DOI: http://dx.doi.org/10.5272/jimab.2016223.1253 Received: 08/06/2016; Published online: 08/08/2016 Correspondence to: Milena Karcheva, MD, PhD; Department of Epidemiology, Medical University-Pleven, Bulgaria; e-mail: milena_karcheva@abv.bg 1256 http://www.journal-imab-bg.org / J of IMAB. 2016, vol. 22, issue 3/
Journal of IMAB ISSN: 1312-773X http://www.journal-imab-bg.org http://dx.doi.org/10.5272/jimab.2016223.1257 Journal of IMAB - Annual Proceeding (Scientific Papers) 2016, vol. 22, issue 3 EFFECTIVENESS OF NON-SURGICAL PERIODONTAL THERAPY BY GINGIVAL EXPRESSION OF IL-1β AND IL-6 Antoaneta Mlachkova 1 , Velitchka Dosseva-Panova 1 , Christina Popova 1 , Maya Kicheva 2 1) Department of Periodontology, Faculty of Dental Medicine, Medical University of Sofia, Bulgaria; 2) PhD, biochemist, Progene Ltd., Sofia SUMMARY: Chronic periodontitis is a multifactorial disease that is characterized by attachment loss and loss of alveolar bone. Periodontal pathogens from the subgingival microbiota are suggested as a major etiological factor for the periodontitis. The host inflammatory response against bacteria and their virulence factors underlies the current understanding of the pathogenesis of chronic periodontitis. The destructive immune response was shown to be associated with an extremely increased production of inflammatory cytokines in periodontal tissues. The expression of several cytokines likeIL-1β and IL-6 is responsible for alveolar bone resorption, and loss of connective tissue attachment in chronic periodontitis. Authors reported a reduction in the levels of inflammatory cytokines after successful periodontal therapy. Measuring the levels of inflammatory factors may be diagnostic approach to assess the effectiveness of the therapy of periodontitis. Keywords: chronic periodontitis, cytokines, IL-1β, IL-6, periodontal therapy, periodontal diagnosis. INTRODUCTION: Inflammatory periodontal diseases are related with a response to complex dental biofilm that result in destruction of connective tissue attachment and alveolar bone [1, 2, 3, 4]. Different periodontal diseases showed some variations in the histological and biochemical characteristics, but the molecular mediators and destructive mechanisms of pathological processes are similar. In the gingival tissue and the gingival crevicularfluid of patients with chronic periodontitis were detected high levels of pro-inflammatory factors: RANKL - (Receptor activator of NF- KB ligand), pro-inflammatory cytokines such as: IL-1β and IL-6 and TNF-α [1, 5, 6]. Studies on the expression levels of IL-1β and IL-6 in relation to the periodontal treatment will lead to: completing the understanding of the pathogenesis of periodontal destruction, assessing the severity of periodontitis, assessing the effectiveness of therapy or refractory, prediction of a recurrence and determination of progression of periodontitis. There is evidence that non-surgical therapy of chronic periodontitis is effective leading to reduction of bacteria load and pathogenic species levels in periodontal pockets, elimination of inflammation and significant reduction of periodontal pockets depth. This also results in a shift of the local destructive response including expression levels of some pro - inflammatory factors IL- 1β and IL-6. Their evaluation may serve to evaluate the effectiveness of nonsurgical therapy [1, 2, 6, 7]. AIM: ‣ Assessment of the effectiveness of non-surgical periodontal therapy in chronic periodontitis IL-1β and IL- 6 gingival gene expression. MATERIALS AND METHODS: In this study were used the following assessment methods: 1. Clinical methods: Selection of patients - 20 patients with diagnosis chronic moderate to severe periodontitis. Including criteria: • presence of a minimum of 20 teeth, • presence of periodontal pockets with PPD ≤7 mm to ≥7 mm, • patients without periodontal therapy in the past 6 months, • patients without systemic antimicrobial treatment in last 6 months. Survey excluded: pregnant women, patients with systemic diseases and systemic medication. Clinical parameters: Hygiene Index (HI); Bleeding on probing (BOP); Periodontal pocket depth (PPD); Clinical attachment loss (CAL); Gingival recession (R); Furcation degree - Hamp 1975 classification (F). 2. Molecular methods: ‣ Gene expressionof IL-6 and IL-1β in the gingival tissue was determined by comparative quantitative analysis - TAQ MAN REAL-TIME PCR - (REVERSE TRAN- SCRIPT PCR) method. / J of IMAB. 2016, vol. 22, issue 3/ http://www.journal-imab-bg.org 1257
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