JofIMAB-2016-vol22-issue3

Journal of IMAB
ISSN: 1312-773X
http://www.journal-imab-bg.org
http://dx.doi.org/10.5272/jimab.2016223.1257
Journal of IMAB - Annual Proceeding (Scientific Papers) 2016, vol. 22, issue 3
EFFECTIVENESS OF NON-SURGICAL
PERIODONTAL THERAPY BY GINGIVAL
EXPRESSION OF IL-1β AND IL-6
Antoaneta Mlachkova 1 , Velitchka Dosseva-Panova 1 , Christina Popova 1 , Maya
Kicheva 2
1) Department of Periodontology, Faculty of Dental Medicine, Medical University
of Sofia, Bulgaria;
2) PhD, biochemist, Progene Ltd., Sofia
SUMMARY:
Chronic periodontitis is a multifactorial disease that
is characterized by attachment loss and loss of alveolar
bone. Periodontal pathogens from the subgingival
microbiota are suggested as a major etiological factor for
the periodontitis. The host inflammatory response against
bacteria and their virulence factors underlies the current
understanding of the pathogenesis of chronic periodontitis.
The destructive immune response was shown to be associated
with an extremely increased production of inflammatory
cytokines in periodontal tissues. The expression of
several cytokines likeIL-1β and IL-6 is responsible for alveolar
bone resorption, and loss of connective tissue attachment
in chronic periodontitis.
Authors reported a reduction in the levels of inflammatory
cytokines after successful periodontal therapy.
Measuring the levels of inflammatory factors may be diagnostic
approach to assess the effectiveness of the therapy
of periodontitis.
Keywords: chronic periodontitis, cytokines, IL-1β,
IL-6, periodontal therapy, periodontal diagnosis.
INTRODUCTION:
Inflammatory periodontal diseases are related with
a response to complex dental biofilm that result in destruction
of connective tissue attachment and alveolar bone [1,
2, 3, 4]. Different periodontal diseases showed some variations
in the histological and biochemical characteristics,
but the molecular mediators and destructive mechanisms
of pathological processes are similar. In the gingival tissue
and the gingival crevicularfluid of patients with
chronic periodontitis were detected high levels of pro-inflammatory
factors: RANKL - (Receptor activator of NF-
KB ligand), pro-inflammatory cytokines such as: IL-1β and
IL-6 and TNF-α [1, 5, 6].
Studies on the expression levels of IL-1β and IL-6
in relation to the periodontal treatment will lead to: completing
the understanding of the pathogenesis of periodontal
destruction, assessing the severity of periodontitis, assessing
the effectiveness of therapy or refractory, prediction
of a recurrence and determination of progression of
periodontitis.
There is evidence that non-surgical therapy of
chronic periodontitis is effective leading to reduction of
bacteria load and pathogenic species levels in periodontal
pockets, elimination of inflammation and significant reduction
of periodontal pockets depth. This also results in a shift
of the local destructive response including expression levels
of some pro - inflammatory factors IL- 1β and IL-6. Their
evaluation may serve to evaluate the effectiveness of nonsurgical
therapy [1, 2, 6, 7].
AIM:
‣ Assessment of the effectiveness of non-surgical
periodontal therapy in chronic periodontitis IL-1β and IL-
6 gingival gene expression.
MATERIALS AND METHODS:
In this study were used the following assessment
methods:
1. Clinical methods: Selection of patients - 20 patients
with diagnosis chronic moderate to severe periodontitis.
Including criteria:
• presence of a minimum of 20 teeth,
• presence of periodontal pockets with PPD ≤7 mm
to ≥7 mm,
• patients without periodontal therapy in the past 6
months,
• patients without systemic antimicrobial treatment
in last 6 months.
Survey excluded: pregnant women, patients with
systemic diseases and systemic medication.
Clinical parameters:
Hygiene Index (HI); Bleeding on probing (BOP);
Periodontal pocket depth (PPD); Clinical attachment loss
(CAL); Gingival recession (R); Furcation degree - Hamp
1975 classification (F).
2. Molecular methods:
‣ Gene expressionof IL-6 and IL-1β in the gingival
tissue was determined by comparative quantitative analysis
- TAQ MAN REAL-TIME PCR - (REVERSE TRAN-
SCRIPT PCR) method.
/ J of IMAB. 2016, vol. 22, issue 3/ http://www.journal-imab-bg.org 1257