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Syllabus - MBBS

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Microbiology 37<br />

3. use the correct method of collection, storage and transport of clinical material for microbiological<br />

investigations<br />

COURSE CONTENT<br />

III SEMESTER<br />

1. Introduction to Microbiology<br />

(i) Natural history of microbial diseases.<br />

(ii) Unique differentiating features of eukaryotes and prokaryotes<br />

(iii) Source and spread of microbes<br />

(iv) Rationale for classifying microbes into bacteria, fungi viruses, parasites.<br />

2. Introduction to Bacteriology<br />

– The nature of bacteria<br />

– Morphological differences<br />

– Growth requirement<br />

– Nomenclature and classification<br />

– Enumeration of bacteria responsible for certain primary diseases.<br />

3. Bacterial Staining and Cultivation<br />

– Microscopy : types and principles<br />

– Staining : principles<br />

– Media for growth / bacterial colony<br />

– Familiarization with materials used<br />

4. Common Tests for Bacterial identification<br />

– Various types of staining such as simple, differential staining; different procedures of staining<br />

and their principles<br />

– Motility testing<br />

– Common sugar fermentation and other biochemical tests such as Catalase / Coagulase/ citrate<br />

utilization/ nitrate reduction / urease/ PPA/ OF/ Indole etc.<br />

– Sensitivity testing<br />

5. Introduction to parasitology<br />

– Biology of protozoa<br />

– Protozoan parasites causing human infection<br />

– Medically important helminths<br />

– Ectoparasites<br />

6. Introduction to Virology<br />

– The nature and properties of viruses<br />

– Classification of viruses

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