Syllabus - MBBS

40 Syllabus MBBS — AIIMS
(To be continued in IV Semester)
PRACTICAL SCHEDULE FOR III SEMESTER
1. Microscopy and micrometry
– Introduction to microscopes
– Focussing slides under low/ high power and oil immersion
– Principles and demonstration of various types of microscopes
2. Direct demonstration of bacteria by staining
– Gram staining
– Albert’s staining
– Acid fast staining
3. Motility tests and biochemical tests for bacterial identification
– Hanging drop method for motility testing
– Important biochemical tests : principles and interpretation
4. Laboratory diagnosis of viral infections
– Collection and transport of samples
– Demonstration of egg inoculation techniques, cell culture, cytopathic effect, plaque assay,
serological tests (complement fixation, haemagglutination inhibition, neutralization, ELISA)
5. Laboratory diagnosis of fungal infections
– Collection and transport of specimens
– Gram stain, KOH preparation, India ink preparation for direct demonstration
– Sabouraud’s dextrose agar/media
– Lactophenol cotton blue for identification
– Latex agglutination test
6. Sterilization and disinfection
– Visit to media and sterilization room (demonstration of autoclave and hot air oven)
– Visit to CSSD
7. Stool examination for cysts
– Collection and transport of stool sample for parasites
– Direct examination (saline and iodine preparations)
– Concentration of stool for parasites
– Identification of cysts
III SEMESTER - SCHEDULE BREAK-UP
Didactic lectures (DL) : 16
16 hours
Intra-departmental seminars (IDS) : 3 6 hours
Integrated seminars (IS) : 2
4 hours