2017 Cardiovascular Research Day Abstract Book

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67 An XX Sex Chromosome Complement Promotes Hypercholesterolemia and Atherosclerosis in Ldlr-/- Mice Fed Western Diet Yasir Alsiraj, MS 1 • Sean Thatcher, PhD 1 • Lei Cai, PhD 2 • Ryan Temel, PhD 2 • Patrick Tso, PhD 3 • Lisa Cassis, PhD 1 1Pharmacology and Nutritional Sciences, University of Kentucky • 2 Saha <strong>Cardiovascular</strong> <strong>Research</strong> Center, University of Kentucky • 3 Department of Pathology and Laboratory Medicine, University of Cincinnati Graduate Student Objectives: Sex hormones are primary contributors to sexual dimorphism of cardiovascular diseases, but little is known about the influence of genes on sex chromosomes as mediators of cardiovascular sex differences. In this study, we hypothesized that genes on sex chromosomes influence the development of hypercholesterolemia and atherosclerosis. Methods and Results: Transgenic male mice with deletion of Sry from the Y-chromosome expressing Sry on autosomes (8-12 weeks of age) were bred to female Ldlr-/- mice to generate female and male mice with an XX or an XY sex chromosome complement. Male (M) and female (F) mice were fed a Western diet (Teklad TD88137) for 3 months. XX mice exhibited increased body weights compared to XY mice, regardless of gonadal sex (FXX, 38 ± 1.8; FXY, 30.6 ± 1.3 g; P
68 Adipocyte deficiency of ACE2 increases systolic blood pressures of obese female C57BL/6 mice Robin Shoemaker, PhD 1 • Wen Su, MD 2 • Ming Gong, PhD 2 • Lisa Cassis, PhD 1 1Pharmacology and Nutritional Sciences, University of Kentucky • 2 Physiology, University of Kentucky Postdoc Background: We demonstrated that sexual dimorphism of obesity-hypertension was associated with differential activity of adipocyte angiotensin-converting enzyme 2 (ACE2) in male versus (vs) female mice. These data suggest that adipocyte ACE2 regulates blood pressure by influencing the balance of angiotensin II (AngII, a substrate of ACE2) vs angiotensin-(1-7) (Ang-(1-7)), which differs in male and female mice. We hypothesized that deficiency of ACE2 in adipocytes increases blood pressure of HF-fed female mice. Methods/Results: Mice with adipocyte ACE2 deficiency were developed from breeding Ace2fl/fl mice to transgenic C57BL/6 mice with heterozygous transgenic expression of Cre recombinase driven by the adiponectin promoter (Ace2Adipo). Female or male Ace2fl/fl and Ace2Adipo mice (8 weeks old) were fed a HF (60% kcal as fat) or low fat (LF; 10% kcal from fat) diet for 16 weeks, after which blood pressure was quantified by radiotelemetry. Systolic blood pressures (SBP) were not different in LF-fed female mice of either genotype (24 hr average SBP [mmHg]: Ace2fl./fl: 121+/-1; Ace2Adipo: 121+/-1; p>0.05). However, SBP was significantly increased in HF-fed female Ace2Adipo vs Ace2fl/fl mice (Ace2fl/fl: 127+/-2; Ace2Adipo: 133+/-3; p
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Cardiovascular Research Day ABSTRAC
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SCHEDULE FOR THE DAY Friday, Novemb
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SCHEDULE FOR THE DAY continued Frid
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2017 GILL HEART INSTITUTE YOUNG INV
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FEATURED SPEAKER Calum MacRae, M.D.
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SPECIAL PRESENTATION Mark Stoops He
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NOTES 12
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2017 POSTER PARTICIPANTS 40 Mohamed
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2017 ABSTRACTS 16
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2 Inhibition of Megalin Reduces Ath
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4 Identification of a Lipid Signatu
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6 Serum Amyloid A Activates the NLR
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8 Azithromycin Therapy Reduces Card
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10 Evidence of Angiotensin II-depen
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12 Auditory Entrainment of Respirat
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14 Cardiac specific Rad knockout In
Page 33 and 34: 16 Hypercholesterolemia Induces Acu
Page 35 and 36: 18 A Novel Approach for Modifying I
Page 37 and 38: 20 Clinical Use of the Amplatzer Se
Page 39 and 40: 22 Regulation of Akt Signaling by N
Page 41 and 42: 24 Vascular Inflammatory Regulation
Page 43 and 44: 26 Vascular inflammation induced ex
Page 45 and 46: 28 Sexual Dimorphism of Angiotensin
Page 47 and 48: 30 Computational modeling of amylin
Page 49 and 50: 32 PCB 126 Exposure Increases Perip
Page 51 and 52: 34 Endothelial Mineralocorticoid Re
Page 53 and 54: 36 Optimization of immunomagnetic s
Page 55 and 56: 38 Gestational Diabetes Provokes Po
Page 57 and 58: 40 The Prognostic Role of Elevated
Page 59 and 60: 42 Lysophosphatidic Acid Receptor 4
Page 61 and 62: 44 Recapitulating In Vivo Fibroblas
Page 63 and 64: 46 Sex-Specific Differences in Card
Page 65 and 66: 48 Ticagrelor Reduces Inflammation
Page 67 and 68: 50 Association between Plasma Chole
Page 69 and 70: 52 Does Light Pollution Affect the
Page 71 and 72: 54 Impact of miR-33 antagonism on m
Page 73 and 74: 56 Isolation and characterization o
Page 75 and 76: 58 The XY sex chromosome complement
Page 77 and 78: 60 CHROME: a Long Non-coding RNA th
Page 79 and 80: 62 Making Good: Secretory Quality C
Page 81 and 82: 64 Thrombospondin 1 (TSP1) Deficien
Page 83: 66 Serum Amyloid A3 is a High Densi
Page 87 and 88: 70 Hypercholesterolemia-induced end
Page 89 and 90: 72 Increased Circulating Trimethyla
Page 91 and 92: 74 Circulating Bacterial Small RNA
Page 93 and 94: 76 Pancreatic Beta Cell Export of m
Page 95 and 96: 78 Understanding inhibition of lipo
Page 97 and 98: 80 Insulin signaling regulates apol
Page 99 and 100: 82 Protease-activated Receptor 2 De
Page 101 and 102: 84 Reduced Low-Density Lipoprotein
Page 103 and 104: 86 Pharmacological inhibition of Hu
Page 105 and 106: 88 The Effects of Hypercholesterole
Page 107 and 108: 90 Reduced HDL in mice overexpressi
Page 109 and 110: 92 Human Antigen R (HuR) Regulates
Page 111 and 112: 94 Impact of miR-33 antagonism on c
Page 113 and 114: 96 HB-EGF is a Key Positive Regulat
Page 115 and 116: 98 HDL-miR-223 Communication Pathwa
Page 117 and 118: 100 Short-Term Exercise Training Di
Page 119 and 120: 102 The Ral-Exocyst Pathway Regulat
Page 121 and 122: NOTES 120
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2017 Cardiovascular Research Day Abstract Book

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