Management of stage Ⅳ rectal cancer - World Journal of ...

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A SS 0 1023 ed into the abdominal cavity of AHF rats. The mortality rate and hepatic pathological changes were determined. At 48 h after transplantation, HE staining of the encapsulated group revealed that the hepatic lobules were still intact; denaturation was the major change in hepatocytes and the area of necrosis nidus was small, and congestion and hemorrhage were almost undetectable (Figure 5). The mortality rate at 48 h after transplantation in three groups was 42.1% (encapsulated group), 65% (unencapsulated group) and 75% (PBS group), respectively. Compared with the unencapsulated group, the mortality rate of the encapsulated group was significantly lower (P < 0.05). In addition, the serum biochemical indexes of ALT, AST and total bilirubin in the microencapsulated group differed significantly from those in the PBS group (P < 0.01) WJG|www.wjgnet.com C 91.2% 10 0 10 1 CD34-PE 10 2 10 3 FS 10 0 10 1 Figure 1 FACS determination of CD34 + cells. A: Purity of CD34 + cells; B: Homotypic control cells. A B C D Zhang FT et al . Transplantation of microencapsulated hepatic-like cells B 0 1023 H 0.2% CD34-PE 50 μm 100 μm 50 μm 50 μm Figure 2 Cell culture and analyses. A: After 16 d; B: A binucleated cell; C, D: Positive staining for albumin (C) and α-fetoprotein (D) after 16 d of indction. at 48 h after transplantation, but there were no differences between the encapsulated and the unencapsulated group (Table 2). At 1-2 wk post-transplantation, free microcapsules with a round clear structure and a smooth surface were observed in peritoneal lavage fluid, surviving cells in microcapsules were found by trypan blue staining, but some fibrous tissues around microcapsules were also detected in the greater omentum of encapsulated group by HE staining (Figure 6). DISCUSSION 10 2 With the continued increase in people with hepatic failure from cirrhosis and hepatocarcinoma, cell transplantation as an effective therapy is becoming a matter of concern 941 February 21, 2011|Volume 17|Issue 7| 10 3
Zhang FT et al . Transplantation of microencapsulated hepatic-like cells Table 2 Changes in serum biochemical indexes at different times 48 h after injection D-GalN All 3 groups Encapsulated group Unencapsulated group ALT (U/L) 3242.3 ± 2403.24 93.93 ± 63.45 b AST (U/L) 4237.20 ± 1372.07 168.87 ± 89.33 b TBIL (μmol/L) 5.57 ± 1.86 1.73 ± 1.01 a WJG|www.wjgnet.com 48 h after transplantation 7 d after transplantation PBS group Encapsulated group Unencapsulated group PBS group 126.1 ± 54.35 245.9 ± 67.87 42.25 ± 11.86 45.07 ± 10.56 47.27 ± 11.08 275.7 ± 52.74 439.7 ± 133.01 162.6 ± 54.29 124.52 ± 24.61 114.83 ± 16.50 2.23 ± 1.98 3.50 ± 1.23 1.90 ± 0.52 2.72 ± 0.96 3.72 ± 1.18 Data are shown as means ± SD. a P < 0.05; b P < 0.01, in comparison with PBS group. TBIL: total bilirubin; AST: Aspartate aminotransferase; ALT: Alanine aminotransferase. ALB GATA-4 AFP GAPDH d 0 d 8 d 16 Figure 3 Reverse transcription-polymerase chain reaction analysis of umbilical cord blood CD34 + cells cultured in vitro d 0, d 8 and d 16. ALB: Albumin; AFP: α-fetoprotein. A B C D ALB (μg/mL) 48 h 100 80 60 40 20 0 With growth factors Without growth factors 0 8 16 Figure 4 Determination of albumin expression by Enzyme-Linked Immunosorbent Assay. ALB: Albumin. 100 μm 100 μm Figure 5 Pathological changes in the livers of acute hepatic failure rats. A: Liver at 48 h after injection of D-galactosamine; B: Liver at 48 h after microcapsule transplantation; C: HE staining of the liver shown in section (A); D: HE staining of the liver shown in section (B). 942 February 21, 2011|Volume 17|Issue 7| t/d
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World Journal of Gastroenterology W
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Robert JL Fraser, Daw Park Jacob Ge
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Italy Donato F Altomare, Bari Piero
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Fernando Azpiroz, Barcelona Ramon B
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Contents BRIEF ARTICLE WJG|www.wjgn
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Online Submissions: http://www.wjgn
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expressed miRNAs including known on
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Oh et al [73] have shown that upreg
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5 Bardeesy N, DePinho RA. Pancreati
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Fazli L, Motoo Y, Wang Q, Rocchi P,
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standard investigation to confirm t
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proximity to a T3 tumor or nodules
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CONCLUSION Imaging in rectal cancer
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Ronnekleiv-Kelly SM et al . Palliat
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Popek S et al . Neoadjuvant vs adju
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esection [19] . Conversely, approxi
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that option again, than patients wh
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Mulsow J et al . Treatment options
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most significant is ensuring oncolo
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tive times and concerns over both l
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Sharma C et al . Clinical advances
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Table 10 Published results on lapar
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Page 85 and 86: Sharma C et al . Clinical advances
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Page 96 and 97: Table 2 Neuronal density and mean a
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Page 100 and 101: troparesis. BMC Gastroenterol 2008;
Page 104 and 105: Table 2 Details of patients who nee
Page 106 and 107: Table 5 Lab data and performance st
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Page 112 and 113: A BMI 28 26 24 22 20 18 40-49 50-59
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Page 116 and 117: Part1. Diagnosis and classification
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Page 120 and 121: of osteomalacia with oral 25-hydrox
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Page 124 and 125: Table 1 Comparisons of three routes
Page 126 and 127: study demonstrated that the water-j
Page 128 and 129: It has been recently shown that CD1
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Page 134 and 135: J Gastroenterol 2011; 17(7): 938-94
Page 138 and 139: A B C D for more scientists. Cell t
Page 140 and 141: marrow as a potential source of hep
Page 142 and 143: INTRODUCTION Hepatocellular carcino
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Page 147 and 148: Liu QY et al . Primary clear cell c
Page 149 and 150: Online Submissions: http://www.wjgn
Page 151 and 152: Instructions to authors Indexed and
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