Flower development of Lilium longiflorum - The Lilium information ...
Flower development of Lilium longiflorum - The Lilium information ...
Flower development of Lilium longiflorum - The Lilium information ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
SUMMARY<br />
Summary<br />
Lily (<strong>Lilium</strong> spp.) is among the most traditional and beloved ornamental<br />
flowers worldwide. <strong>The</strong> genus <strong>Lilium</strong> comprises almost one hundred species, among<br />
which is the primary subject <strong>of</strong> our research, described in this thesis, the species <strong>Lilium</strong><br />
<strong>longiflorum</strong> (Thunb.), known as trumpet lily or Easter lily.<br />
Despite the great economic importance <strong>of</strong> ornamental lily species, little is<br />
known about its biology at the molecular level so far. In a time when two genomes are<br />
fully sequenced, Arabidopsis thaliana and Oryza sativa, only a few genes have been<br />
characterized in <strong>Lilium</strong> spp. yet. Possible reasons for this are discussed throughout this<br />
thesis.<br />
This work intends to be a contribution to bridging the fundamental research<br />
concerning transcription factors involved in <strong>development</strong> <strong>of</strong> flower morphology in<br />
model species and the applied objectives <strong>of</strong> molecular breeding for manipulating<br />
flower morphology, endeavouring to create new cultivars with specific and novel<br />
features, more specifically in <strong>Lilium</strong> spp.<br />
<strong>The</strong> ABC model for floral <strong>development</strong> was proposed more than 10 years ago<br />
and since then many studies have been performed in model species, such as<br />
Arabidopsis thaliana, Antirrhinum majus, petunia and many other species in order to<br />
confirm this model. This investigation has led to additional <strong>information</strong> on flower<br />
<strong>development</strong> and to more complex molecular models.<br />
In the first chapter <strong>of</strong> this thesis, notions <strong>of</strong> molecular floral <strong>development</strong>, the<br />
difficulties <strong>of</strong> working with molecular biology <strong>of</strong> lily, the state-<strong>of</strong>-the-art in lily<br />
transformation are introduced, as well as general overviews <strong>of</strong> transcription factors,<br />
MADS-box genes, the ABCDE model for flower <strong>development</strong> and functional<br />
characterization <strong>of</strong> genes in heterologous systems. <strong>The</strong>se concepts will guide the<br />
reader throughout the work we present here.<br />
AGAMOUS (AG) is the only C type gene found in Arabidopsis and it is<br />
responsible for stamen and carpel <strong>development</strong> as well as floral determinacy. In the<br />
second chapter, we describe the isolation <strong>of</strong> LLAG1, a putative AG orthologue from<br />
lily (L. <strong>longiflorum</strong>) by screening a cDNA library derived from developing floral buds.<br />
<strong>The</strong> deduced amino acid sequence <strong>of</strong> LLAG1 revealed the MIKC structure and a high<br />
homology in the MADS-box among AG and other orthologues. Phylogenetic analysis<br />
indicated close relationship between LLAG1 and AG orthologues from monocot<br />
species. Spatial expression data showed LLAG1 transcripts exclusively in stamens and<br />
125