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Flower development of Lilium longiflorum - The Lilium information ...

Flower development of Lilium longiflorum - The Lilium information ...

Resumo metodológica,

Resumo metodológica, além da rapidez de obtenção dos resultados. Estas características aludem ao sistema VIGS como um instrumento alternativo na genômica funcional, mesmo quando experimentos são desejados em larga escala. Este sistema já está bem estabelecido em Nicotiana benthamiana, mas esforços estão sendo atualmente alocados na melhoria do sistema VIGS em outras espécies, incluindo monocotiledôneas. A pesquisa neste campo tem-se focado atualmente em revelar os mecanismos do VIGS e do silenciamento gênico pós-transcricional (PTGS ou posttranscriptional gene silencing), bem como encontrar novos vetores virais no intuito de aumentar o espectro de espécies hospedeiras. No capítulo sete, discutem-se as vantagens de se utilizar o sistema VIGS para acessar funções gênicas em plantas. Apresentam-se os mecanismos moleculares envolvidos no processo, os elementos metodológicos disponíveis como vetores, procedimentos de inoculação, com exemplos nos quais o sistema foi aplicado com sucesso na caracterização da função de genes de plantas. Ademais, analisa-se o potencial da aplicação do sistema VIGS na caracterização funcional de fatores de transcrição envolvidos no florescimento de espécies de monocotiledôneas. As análises de funções gênicas envolvidas no desenvolvimento floral de lírio e a geração de informações úteis no melhoramento molecular potencial deste espécie foram os principais objetivos do trabalho descrito nesta tese. O campo de estudos dos aspectos moleculares do desenvolvimento floral de lírio está agora de portas abertas e o futuro poderá trazer aspectos muito interessantes que contribuam para levar novas ferramentas aos melhoristas de espécies ornamentais, bem como revelar características particulares do florescimento das monocotiledôneas e da família Liliaceae. 136

ACKNOWLEDGEMENTS Acknowledgements With a feeling of mission accomplished and great expectations for my return to Brazil, I can finally express my deep appreciation towards those that supported me during the preparation of this thesis and contributed, in so many ways, to this happy ending. I start writing these pages with an immense rejoicing. I would like to initiate the expression of my gratitude to the first people that opened to me the doors of the Old Continent. My dear friend, Francine Lorena Cuquel, who introduced me to Raoul Bino, during a congress in Piracicaba, the city where I did my M.Sc. program. She knew that I was looking for an opportunity to go abroad and extend my experiences on plant molecular biology. Raoul introduced me to Huub Löffler, which in turn introduced me to my former supervisor Jurriaan Mes, my copromoter Gerco Angenent and my professor Sacco de Vries. Later, as the institutional rearrangement took place at PRI, Frans Krens received me as his Ph.D. student. Here, I want to pronounce my admiration to Sacco, who taught me to face Science with a great spirit, always keeping the humour and, through his attitude, teaching me that Science can also be fun! Gerco introduced me to the MADS world, and showed me that a brain can accumulate an amazing amount of information. Frans, with his accurate scientific method, good administration skills and gentleness, showed me that a person could be strong without ever losing the tenderness. You not only made this thesis possible, guiding me through the mists of doubts and uncertainties, but also made me a better scientist and improved my spirit. In order to get a financial support from the Brazilian government through CNPq, some people believed in me, assuring that I was capable of successfully following a Ph.D. program. Those scientists were José Édson Fontes Figueiredo, Luiz Antonio dos Santos Dias and Francine Lorena Cuquel. Thanks you all for the support, friendship and example! As soon as I was introduced in the former Genetics and Breeding Business Unit at PRI, I was welcomed with an open heart by many people, especially Jurriaan, Huub, Bernadette (to whom I own my first experiences with lily tissue culture and transformation), Greetje, Betty, Ton, Ria Jongerius, Linda, Ria Vrielink, Herma, Danny, Ludmila, Chris, Sjaak, Erik, Karin, Marcel, Jelle, and later by Jaan, Elma, Marjan, Iris, Mark, Koen (with whom I discussed many of the constitutive weird results), Theo (thanks for bringing me the nice lily mutant, which I report in chapter 6), Kitty, Dianka, Carole, Alex, Paul, Wang. Cheers for all the support I received also from the colleagues Lim, Si-Jun (with whom I gladly share the office), Ronald, Zifu and Paulo (thanks for the friendship, political discussions, gatherings and lunches 137

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