L - Cochin University of Science and Technology
L - Cochin University of Science and Technology
L - Cochin University of Science and Technology
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2. 2. 2. 1. Macrobenthos<br />
After sub sampling, all the samples remaining in the grab were sieved through<br />
O.5mm (SOO J!) sieve to separate specimens from the substrate. For unloading the<br />
sediment from the grab <strong>and</strong> then sieving, a wooden platform was fabricated (Plate 4).<br />
The sediment was unloaded in the conical aluminum portion <strong>of</strong> the platfonn, which<br />
was attached to the upper frame. The lower frame was modified in such a way so as<br />
to slide in <strong>and</strong> out the sieve in the form <strong>of</strong> a drawer, where the sediment unloaded as<br />
directly collected in the sieve. For the present study, O.S mm sieve was selected for<br />
separating macro benthos.<br />
In order to avoid sample degradation, the sieving was accomplished on board<br />
soon after the sample collection. It was done by washing the sample with seawater in<br />
a bucket <strong>and</strong> then sieving using SOOJ! sieve. Since immediate processing for<br />
taxonomic study is not possible, sieved specimens <strong>and</strong> residual sediments were<br />
transferred to plastic bottles <strong>and</strong> fixed in S% neutral fonnaldehyde with "Rose<br />
Bengal" for staining the live organisms. Samples were properly labeled with details<br />
like cruise number, date, time, station position <strong>and</strong> depth.<br />
2.2.2.1. Meiobenthos<br />
At each station, with the help <strong>of</strong> a glass corer (2.S cm diameter), sediment<br />
samples <strong>of</strong> 10 cm long cores were drawn <strong>and</strong> length <strong>of</strong> the core measured. Replicate<br />
(in some cases only one) sub samples were collected from each haul. The samples in<br />
Toto were transferred to polythene containers, labeled <strong>and</strong> material preserved in 70%<br />
alcohol-Rose Bengal solution for further examination.<br />
On arrival at the shore laboratory, the sediment samples were processed<br />
through a set <strong>of</strong> two sieves, the upper one <strong>of</strong> SOOJ! <strong>and</strong> the lower with 63Jl. Residue<br />
retained over 63 J! sieve was back washed into a glass container <strong>and</strong> the same<br />
preserved in 70% alcohol or 4% neutral formalin. In some cases, Rose Bengal was<br />
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