Stave River Water Use Plan - BC Hydro

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Stave River Water Use Plan Monitoring Terms of Reference June 13, 2005 To analyse total phosphorous (TP) content, the TP sample test tubes and caps (one per site) will first be rinsed with the sampled water, and then filled, capped and labelled. At no time will the mouth of the bottle or the inside of the cap be touched, as it can easily become contaminated. Once filled, the sample test tubes should be placed in a cooler and then refrigerated until analysed. Once per field trip, two sample bottles of double distilled water (DDW) will be prepared as blanks for comparison purposes. Figure 2: Map of Stave Lake and Hayward Lake reservoirs showing monitor sampling locations BC Hydro Page 14
Stave River Water Use Plan Monitoring Terms of Reference June 13, 2005 In preparation for dissolved phosphorus and nitrogen (in the form of N03) analysis, the mixed epilimnetic water sample will be field filtered using a 47 mm filtering manifold equipped with an ashed GF/F filter. Prior to filtering, the filter will be rinsed with 180 ml of DDW, and then rinsed again with 180 ml of the sampled epilimnetic water. Plastic 120 ml sample bottles will be rinsed by filtering 60 ml of the Van Dorn sampled water into each bottle, which will then be capped and shaken. All filtrate to this point will be discarded. The rinsed sample bottles will then be filled with filtered epilimnetic water, capped tightly, and immediately frozen. Once per field trip, two sample bottles of double distilled water (DDW) will be prepared as blanks for comparison purposes. During Phase 1, water quality samples will be collected every 4-6 weeks to determine annual trends. In phase 2, sampling frequency will be reduced to 4 times per year (every 8 weeks during months of May to November). All samples will be to be sent immediately to a certified chemistry laboratory for analysis. For consistency purposes it is recommended that the Department of Fisheries and Oceans chemistry lab in Cultus Lake should be used. The data obtained from this analysis will be used to test hypotheses H01, H02, and H05 to H07 in Section 1.3. 2.3.3 Plankton Phytoplankton Phytoplankton community structure will be determined by visual assessment of water samples collected by vertical Van Dorn sampler at depths 1, 3, and 5 m below the water surface. Each sample will be stored in 250 ml glass bottles to which 2 ml of acidic Lugol’s iodine solution has been added as a preservative. Samples are to be stored in a cool dark location until analysed. Sampling at each site (Figure 2) will occur monthly between March and November each year. Only one sample per site will be taken and analysed. Prior to enumeration by the Utermohl (1958) method, the sample will be shaken for 60 s, poured into 25 ml settling chambers, and then allowed to settle for a minimum of 8 hr. Counts should be done with an inverted phase-contrast plankton microscope. Counting will follow a two step process starting with an enumeration of microphytoplankton (e.g., diatoms, dinoflagellates, and blue-green algae) in the first 5-10 random fields at a microscope magnification of 250X. This will be followed by a random transect (10-15 mm in length) count where magnification is increased to 1560X to enumerate ultra-phytoplankton (e.g., pico-cyanobacteria and nano-flagellates). In total, a minimum of 250 cells will be counted per sample to ensure statistical accuracy. All enumerated cells will be identified to the nearest species taxon level. Counts will be reported as an abundance value (cells·ml -1 ) and in terms of biovolume (mm 3 L -1 ). During phase 1 of the monitor, phytoplankton enumeration will be carried out every 4 to 6 weeks each year to capture seasonal and annual trends. The level of phytoplankton sampling effort will be reduced to just one sample per site, per year for Phase 2 of the monitor. The maximise the utility of the phase 2 samples, it will have to BC Hydro Page 15
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Stave River Water Use Plan - BC Hydro

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