Stave River Water Use Plan - BC Hydro

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Stave River Water Use Plan Monitoring Terms of Reference June 13, 2005 Depth (m) Bench Mark AFDW Sp. Comp. Chl a C Accrual Maximum Reservoir Elevation Minimum Reservoir Elevation Pressure Transducer Figure 2-1: Schematic diagram illustrating the distribution of periphyton sampling blocks along a transect line to measure the pattern of periphyton growth. Inset shows the layout of quadrants on the Plexiglas plates on top of each block. BC Hydro Page 32
Stave River Water Use Plan Monitoring Terms of Reference June 13, 2005 2.3.2 Laboratory Methods Chlorophyll The periphyton scraped from Quadrant 1 will be filtered through a 47 mm diameter, 0.45um Millipore HA filter and rinsed twice with double distilled water (DDW). If periphyton growth is found to be exceptionally heavy, i.e., a predominance of filamentous green or blue-green algae, then a Whatman GF/F filter will be used instead of the Millipore filter. The algae-ladened filters will be placed in acetone overnight to extract the chlorophyll. The resulting solution will then be analysed for chlorophyll content using a spectrophotometer or Turner © fluorimeter. Chlorophyll concentrations will be reported on a per-unit area basis, i.e., mg/cm 2 or m 2 . Chlorophyll data will only be collected during Phase 1 of the monitor. Ash-free dry weight (biomass) The periphyton scraped from Quadrant 2 will filtered onto a pre-ashed and weighed 5.5 cm Whatman GF/F glass fibre filter. After filtration at low vacuum (< 20 cm Hg), the filters will be folded in half, placed in aluminium weighing dishes and frozen. The Filters will then be dried in an oven at 105 o C to constant weight, weighed, then ashed at 500 °C for four hours in a muffle furnace, and then weighed again. Periphyton biomass will be expressed as a dry weight (DW) and ash-free dry weight (AFDW) organic content per unit area, i.e., mg/cm 2 or m 2 . Biomass accrual The rate of accumulation of organic periphyton biomass or accrual will be calculated by dividing total organic biomass (AFDW) accumulation by days in the interval between sampling. Values are then expressed as mg of organic matter·m -2 ·day -1 . For purposes of comparison, the carbon (C) content of periphyton will be calculated as 50 % of the organic content (AFDW) in the sample (Stockner and Armstrong 1971). The accrual rate will be a first approximation of periphyton net production because it does not account for sloughing or grazing losses during the sampling interval (immersion period). During phase 1 of the monitor, biomass accrual will be estimated using samples collected from the 25 cm 2 quadrants. During phase 2 of the monitor however, accrual will be estimated from a single large sample collected at one of the 100 cm 2 quadrants. Species composition Species composition data will only be collected during Phase 1 of the monitor and will consist of two types of analyses; that of the biofilm community and of the attached algae community. Biofilm The ‘Biofilm’ community is mostly microbial (e.g. bacteria, picoplankters, flagellates, ciliates and fungi) and constitutes the first successional sere of the periphyton community in both lakes and streams. A homogeneous 10 ml sub-sample of BC Hydro Page 33
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Stave River Water Use Plan - BC Hydro

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