Landbauforschung vTI Agriculture and Forestry ... - 1. Januar 2008

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132 ai effect of dietary inclusion of REE (0; 200 mg REE-citrate/kg diet) bj effect of the sex axb( ) ij interactions between REE concentration and sex eijk error term. The performance parameters of experiment 2 were analyzed according to a one-way factorial design of analysis of variance (ANOVA) corrected for initial LW using the following model: Y ij = µ + a + i where βy.x(xij - ˉx ..) + eij (2) Y tested parameter of the calf‘‘j’’ fed diet type ‘‘i’’ ij µ overall mean a effect of dietary inclusion of REE (0, 100, 200 and i 300 mg REE-citrate/kg diet) β regression coefficient of y on x y.x x co-variable ij x mean value for x error term e ij Results of AVONA are presented in the tables either as arithmetic means (Experiment 1) or as least square means (LSmeans; Experiment 2) and the pooled standard errors of means (PSEM). In case of significant treatment effects the Tukey-Test for multiple comparisons was used to identify significant mean value differences (p < 0.05). The Statistica for Windows operating system (StatSoft Inc. 2007, Version 10.0) was used for all statistical evaluations. 3 Results 3.1 Composition and REE concentrations of feedstuffs used in experiment 1 and 2 The contents of crude nutrients and the REE (pure elements) concentrations of the analyzed feedstuffs milk replacer, concentrate and roughage as well as the Lancer 500 ® are summarised in Tables 3 and 4. The nutrient composition of the two milk replacers was almost equal and only very small differences in crude nutrients could be detected between control and supplemented concentrates (Tables 3 and 4). Negligible amounts of REE were detected in the unsupplemented milk replacer, whereas 9.5 and 17.0 mg total REE/kg DM were measured in the concentrate of experiment 1 and in the unsupplemented control concentrate of experiment 2. In the supplemented concentrates, total REE concentrations of 43, 90, 124 mg/kg DM were detected and were increased as intended by formulation. Also grass hay and grass silage, which were used as roughage contained low levels of REE (Tables 3 and 4). Table 3: Dry matter, nutrient composition and energy concentration of the milk replacer, concentrates and grass hay as well as the rare earth element (REE) contents in the milk feeding period Milk replacer REE-citrate supplementation [mg/kg DM] 0 200 Concentrate Grass hay REE-Citrate Dry matter [g/kg] Crude nutrients [g/kg DM] 961 961 883 863 Crude ash 78 77 64 62 Crude protein 212 216 234 85 Crude fat 141 142 42 15 Crude fibre - - 67 303 Acid detergent fibre - - 84 318 Neutral detergent fibre - - 200 604 Metabolizable energy [MJ/kg DM] 15.9 15.9 12.6 9.0 Pure rare earth elements [mg/kg DM] Lanthanum 0.1 24 2 0.3 42 243 Cerium 0.1 41 4 0.6 73 395 Praseodymium 0.0 5 0.5 0.1 8 641 Neodymium 0.0 0.02 1.7 0.1 23 Remaining REE* 0.0 1 1.3 0.1 1 977 Total rare earth elements Notes: *Without the element Scandium 0.2 71.02 9.5 1.2 126 279
A. Schwabe, U. Meyer, G. Flachowsky, S. Dänicke / Landbauforschung - vTI Agriculture and Forestry Research 3 2012 (62)129-136 133 Table 4: Dry matter, nutrient composition and energy concentration of the concentrates, grass silage and grass hay as well as the rare earth element (REE) contents in the growing period REE-citrate supplementation [mg/kg DM] Concentrates 0 100 200 300 Grass silage Grass hay REE-citrate Dry matter [g/kg] 889 894 890 889 342 870 Crude Nutrients [g/kg DM] Crude ash 64 64 65 62 100 64 Crude protein 223 216 210 213 150 95 Crude fat 43 43 42 42 36 17 Crude fibre 69 71 69 70 281 306 Acid detergent fibre 88 89 87 89 308 323 Neutral detergent fibre 207 216 213 212 520 609 Metabolizable energy [MJ/kg DM] 12.6 12.6 12.6 12.6 9.5 9.0 Pure rare earth elements [mg/kg DM] Lanthanum 5 13 29 41 0.5 0.2 42 243 Cerium 9 23 51 71 0.9 0.4 73 395 Praseodymium 1 3 6 9 0.1 0.1 8 641 Neodymium 1 2 2 1 0.2 0.1 23 Remaining REE* 1 2 2 2 0.2 0.1 1 977 Total rare earth elements Notes: *Without the element Scandium 17 43 90 124 1.9 0.9 126 279 3.2 Performance parameters 3.2.1 Experiment 1 In the REE group, one male calf died on the 4 th day of trial for unknown reasons and was not included in the statistical analysis. Furthermore, in both groups some calves were treated due to digestive and respiratory problems. The performance parameters of the pre-ruminant calves over the milk feeding period are shown in Table 5. The mean daily REE-citrate intake of the REE group was 111 mg/d or 123 mg/kg total DM. The initial and final weight was similar between the control and REE group and no interaction was observed between group and sex. However, a significant sex effect was observed at the start and the end of the experiment, as the female calves showed lower live weights than the male calves (Table 5). The mean intake of milk replacer and concentrate was not significantly affected by the REE supplementation, although numerically differences could be determined in the concentrate intake. The supplemented animals consumed 42 g/d (13.5 %) less concentrate than the control animals. With regard to the single group means it becomes obvious that this fact mainly result from the supplemented male calves (Table 5). Furthermore, the REE group showed a lower grass hay intake (26.3 %) compared to the control group, but no statistical analysis could be performed because the feed consumption was recorded daily per group. This depression in feed intake was reflected in the daily weight gain of the calves (496 g/day in REE group vs 525 g/day in control group). As a consequence of the lower feed intake the ME- and crude protein intake was influenced in a similar manner. The feed-to-gain ratio and ME-to-gain ratio was similar between the two groups (Table 5). 3.2.2 Experiment 2 The growth experiment 2 comprised the LW range between 108 ± 9 kg and 182 ± 14 kg and lasted approximately 11 weeks in total. During this time, no calf died and no health problems were observed. The average actual intake of REE-citrate/kg DM amounted to 0; 74 ± 7; 149 ± 13 and 225 ± 15 mg for the Group A, B, C, and D, respectively, and differed distinctly from the planned intake. The grass silage, grass hay and concentrate intake was not different among treatments. Although not statistically significant, the calves of Group D (300 mg REE-citrate/ kg DM) consumed less grass silage (2.9 %) and grass hay (12.1 %) than the calves of the control group (Table 6). Hence, the total feed intake was the lowest for Group D with 4.02 kg DM/d followed by the Group C with 4.06 kg and Group B with 4.10 kg daily DM intake. The control
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