Products at a Glance 2024

www.pelobiotech.com Ask

www.pelobiotech.com Ask for PD Collagenase! The products below are defined by the total Wünsch activity per bottle, an assay that uses a peptide substrate to measure the activity of the collagenase catalytic domain. This activity does not provide functional assessment of collagenase activity but it is one of the most consistent and reliable activity measures used by many collagenase suppliers. The table below provides ordering information and a cross reference chart to indicate which PD Collagenase product may meet your needs. The Collagenase Gold product is similar to the PD Collagenase 800 but does not contain any supplemental purified protease. This product enables users to use other proteases or to purchase from VC the same protease used in the PD Collagenase products (BP Protease, catalog # 003-1000) for creation of a custom mixture. Product Catalog # #/Quantity Cross Reference PD Collagenase 100 PD Collagenase 800 Collagenase Gold PB- 011- 1010 PB-011- 1050 PB-011- 1060 1 g Worthington Type 1 or 2 Liberase TM or DH, Blendzyme 1 or 2 1 g Liberase TL, Liberase DL, Liberase H I 1 g Human Islets To isolate sensitive cells such as islets, high purity and fidelity collagenase are essential due to their critical role in enzymatic degradation of the extracellular matrix without damaging the structural and functional integrity of the cells. Successful human islet isolation requires the use of defined collagenase-protease enzyme mixtures, and intact class I and class II collagenase should be used to ensure maximal islet recovery at the lowest dose of enzyme. This is why Collagenase Gold is ideal for islet isolation. Thereby the use of these enzymes which are GMP and AOF can help with the best results. It has been found that excess collagenase may potentially harm islets during the isolation process, emphasizing the need for precise dosing and high purity enzymes to avoid detrimental effects on the isolated cells. Furthermore, studies have demonstrated that islet mass, quality, and purity are determined by the efficacy of islet isolation, which depends on factors such as organ procurement, collagenase digestion, and purification method. This highlights the critical role of high purity collagenase in ensuring the quality and purity of isolated islets. References 1. Breite AG, et al. Transplantation Proceedings 42 (2010); 2052-2054. 2. McCarthy RC, et al. Transplant Proc 40 (2008); 339-342. 3. Wünsch E and Hedrich H-G. Hoppe-Seyler’s Zeitschrift Physiol Chemie 333 (1963);149-151. 4. Mitchell WM and Harrington WF. Methods in Enzymology (1970) 635-642. 5. McCarthy RC, et al. Transplant 91 (2011) 137-145. Scan the QR code to visit the VitaCyte website to get additional protocols and product information. 6

www.pelobiotech.com CIzyme TM Products Product Catalog # Quantity App Quality CIzyme TM Collagenase HA PB-001-1000 2000 Wünsch units Human islets GMP CIzyme TM Collagenase HA PB-001-1050 200 Wünsch units Rodent islets GMP CIzyme TM Collagenase MA PB-001-2020 1100 Wünsch units Porcine islets GMP CIzyme TM Collagenase MA PB-001-2030 2,5 Mill CDA units Human hepatocytes GMP CIzyme TM rCollagenase HI PB-001-4010 1600 Wünsch units Human islets GMP & AOF CIzyme TM Collagenase Gold PB-011-1060 800 Wünsch units Human islets CIzyme TM Collagenase Gold Plus PB-011-2000 >1,500 FALGPA units Human islets from nondiseased organs GMP CIzyme TM Thermolysin PB-002-3000 6 mg Protease GMP & AOF CIzyme TM BP Protease PB-003-1000 1,1 mill neutral protease units Neutral Protease GMP & AOF CIzyme TM BP Protease PB-003-2000 2,3 mill neutral protease units Neutral Protease GMP & AOF Clostripain PB-004-2000 25 mg Protease GMP CIzyme TM RI PB-005-1030 375k CDA U, 75K NP U Rodent islets CIzyme TM AS PB-005-1090 35 W U, 280k NP U Adipose derived stem cells GMP CIzyme TM Hepatocyte Isolation Kit PB-005-1010 2,5 Mill CDA U,2,2 Mill NP U mg Hepatocytes GMP PD Collagenase 100 PB- 011- 1010 1 g PD Collagenase 800 PB-011- 1050 1 g Porcine & rodent hepatocytes Rodent dendritic cells, rat cardiomyocytes, fibroblasts and porcine kidney epithelial cells 7