QUANTA LiteÂ® C1q CIC ELISA - inova

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Intervalo de LeituraO intervalo de leitura do teste é de 1,23 - 100µg Eq/mL.Esquema da Microplaca1 2 3 4 5 6 7 8 9 10 11 12ABCDEFGHSumário do Procedimento1. Pipetar 100µL de cada calibrador, controlo e amostra diluída (1:101) nos poços.Incubar durante 30 minutos.Lavar.2. Adicionar 100µL de conjugado em cada poço.Incubar durante 30 minutos.Lavar.3. Adicionar 100µL de substrato em cada poço.Incubar durante 30 minutos.4. Adicionar 100µL de solução de paragem em cada poço.Ler as absorvâncias a 450nm.QUANTA Lite é uma marca registrada da INOVA Diagnostics, Inc.Copyright 2012 Todos os Direitos Reservados©6
Referências1. Hay FC et al.: Routine assay for the detection of immune complexes of known immunoglobulin classusing solid phase C1q. Clin. Exp. Immunol., 1976; 24: 396-400.2. Stanilova SA, Slavov ES: Comparative study of circulating immune complexes quantity detection by threeassays - CIF-ELISA, C1q-ELISA and anti-C3 ELISA. J. Immunol. Meth, 2001; 253: 13-21.3. Van Hoeyveld E, Bossuyt X: Evaluation of seven commercial ELISA kits compared with the C1q solidphasebinding RIA for detection of circulating immune complexes. Clin. Chem, 2000; 46:283-5.4. Lambert PH: WHO collaborative study for the evaluation of immune complexes in serum. Clin LabImmunol., 1978; 1: 1-15.5. Espinoza LR and Osterland CK.: Circulating immune complexes. Their clinical significance. Futurepublishing Co., 1983.6. Abrass CK et al.: Correlation and Predictive Accuracy of Circulating Immune Complexes with DiseaseActivity in Patients with Systemic Lupus Erythematosus . Arthritis Rheum., 1980; 23: 273-282.7. Protein Reference Unit Handbook of Autoimmunity (3rd Edition) 2004 Ed A Milford Ward. J. Sheldon,GD Wild. Publ. PRU Publications, Sheffield. 14.8. Biosafety in Microbiological and Biomedical Laboratories. Center for Disease Control/National Institute ofHealth, 2007, Fifth Edition.Fabricado por:INOVA Diagnostics, Inc.9900 Old Grove RoadSan Diego, CA 92131United States of AmericaTechnical Service (U.S. & Canada Only) : 877-829-4745Technical Service (Outside the U.S.) : 00+ 1 858-805-7950support@inovadx.comRepresentante Autorizado:Medical Technology Promedt Consulting GmbHAltenhofstrasse 80D-66386 St. Ingbert, GermanyTel.: +49-6894-581020Fax.: +49-6894-581021www.mt-procons.com624620PRT August 2012Revision 37
Page 1 and 2: QUANTA Lite ®Para utilização em
Page 3 and 4: • Calibradores C1q CIC: 5 frascos
Page 5: Distribuição normal da amostraNo.

QUANTA LiteÂ® C1q CIC ELISA - inova

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