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NEW<br />
ENGLAND<br />
BIOLABS<br />
on target. every time.<br />
ShortCut RNAi Kit<br />
NOVEL TECHNOLOGY TO TARGET THE RIGHT GENE FASTER.<br />
500 bp<br />
A) B)<br />
1000 bp<br />
175 bp<br />
From DNA template to<br />
transfection in just 1 day<br />
Heterogeneous population of<br />
siRNA ensures effective<br />
silencing of target gene<br />
Eliminates trial and error<br />
approach of synthetic siRNA<br />
The ShortCut RNAi Kit is a new system<br />
for the in vitro generation of siRNA mixtures<br />
for RNAi experiments in mammalian cells.<br />
The kit includes reagents for both high-yield<br />
in vitro transcription of large dsRNA and<br />
subsequent processing into a heterogeneous<br />
population of siRNA by digestion with ShortCut<br />
RNase III (also available separately) in a novel<br />
reaction buffer containing manganese.<br />
21 bp<br />
ShortCut 0 0.5 1 2 4 8 16<br />
RNase III (µl)<br />
21 bp<br />
— + — + ShortCut<br />
RNase III<br />
siRNA production by ShortCut RNase III: (A) Varying amounts of ShortCut RNase III were incubated<br />
with 2 µg of a 500 bp dsRNA for 20 minutes. (B) dsRNA fragments (1 kb and 175 bp) were digested<br />
with ShortCut RNase III. Digests were analyzed by 20% TBE polyacrylamide gel electrophoresis.<br />
Kennziffer 25 LW 06 · www.biocom.de<br />
ORDERING INFORMATION:<br />
ShortCut RNAi Kit<br />
E2450S . . . . . . . . 100 transfections<br />
available separately:<br />
ShortCut RNase III<br />
M0245S . . . . . . . . 130 units (100 µl)<br />
M0245L . . . . . . . . 650 units (500 µl)<br />
TransPass R1 and R2 RNA Transfection Reagents<br />
M2551S . . . . . . TransPass R1 (polyamine-lipid)<br />
M2552S . . . . . . TransPass R2 (two component)<br />
GFP Silencing in COS-7 Cells:<br />
COS-7 cells co-transfected with a plasmid<br />
expressing GFP in the absence (control)<br />
or the presence of 30 ng (4 nM) of GFP<br />
siRNA prepared using the ShortCut RNAi<br />
Kit. Cells were photographed 48 hours<br />
post-transfection.<br />
IN T ERNE T- BE S T EL L UNGEN, NEUIGK EI T EN UND T ECHNISCHE INF ORMAT IONEN. w w w.neb-online.de<br />
Ihr zuverlässiger Partner für die Molekularbiologie:<br />
New England Biolabs GmbH<br />
Frankfurt/Main Deutschland Tel. 0800/BIOLABS (246-5227) Fax 0800/BIOLABX (246-5229) email: info@de.neb.com<br />
New England Biolabs Inc.<br />
Ipswich, MA USA 1-800-NEB-LABS Tel. (978) 927-5054 Fax (978) 921-1350 email: info@neb.com internet: www.neb.com<br />
control ShortCut siRNA (4 nM)<br />
the leader in enzyme technology
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