Phenobarbital and its Sodium Salt - IARC Monographs on the ...

Table 3 (contd)
Strain (sex) Initiator, dose, route
<strong>and</strong> duration
Fischer 344
(M)
Fischer 344
(M)
Fischer 344
(M)
Fischer 344
(M)
NDEA, 150 mg/kg bw,
ip × 2
NDEA, 100 mg/kg bw,
ip × 1/week, 3 weeks
NDEA, 200 mg/kg bw,
ip; 2 weeks later, Dgalactosamine,
300
mg/kg bw, ip at the
end of weeks 2 <strong>and</strong> 5
NDEA, 200 mg/kg ip,
PH at week 3
Interval
between
initiator <strong>and</strong>
promoter
Dose <strong>and</strong> duration of PB or
NaPB <strong>and</strong> route
4 months PB, 10, 100 or 500 mg/kg
diet for 7, 30 or 60 days
1 week PB, 500 mg/kg diet, 20
weeks or PB + 100 mg/kg
diet MMTS, 20 weeks
2 weeks PB, 500 mg/kg diet, weeks
3–8
2 weeks PB, 1, 2, 4, 7.5, 15 or 500
mg/kg diet, 6 weeks
PB, 0.01, 0.1 or 0.5 mg/kg
diet, 6 weeks
PB, 1, 2, 4, 7.5, 15, 30, 60,
125, 250 or 500 mg/kg diet,
6 weeks
Promoting or anti-promoting effects
on preneoplastic or neoplastic
hepatocellular lesions
Eosinophilic foci; growth at 100 (7
<strong>and</strong> 60 days), 500 (30 <strong>and</strong> 60 days)
mg/kg diet; at 10 mg/kg diet, PB was
ineffective
GST-P-positive foci; promotion with
PB; inhibition with PB + MMTS
GST-P-positive foci; promotion by
PB; multiple injection of D-galactosamine
as effective as PH
GST-P- <strong>and</strong> TGF-α-positive foci;
promotion at 500 mg/kg diet;
inhibition at 1, 2, 4, 7.5 mg/kg diet
No promotion
GST-positive foci; promotion at
doses higher than 15 mg/kg diet;
inhibition at 2 <strong>and</strong> 4 mg/kg diet
Reference
Kolaja et al.
(1996b)
Sugie et al.
(1997)
Kim et al.
(1997)
Kitano et al.
(1998)
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<strong>IARC</strong> MONOGRAPHS VOLUME 79