methods for impurity profiling of heroin and cocaine - United Nations ...

Annex III 71
Table 4: Retention times for cocaine impurities in the capillary gas
chromatograms in figure V
Peak Compound Retention time (min)
1 Benzoic acid a 2.60
2 Anhydroecgonine methyl ester 3.52
3 Anhydroecgonine a 4.10
4 trans-Cinnamic acid a 4.36
5 Ecgonine methyl ester a 4.94
6 Ecgonine b 5.45
8 Tropacocaine 10.81
9 para-Fluorococaine c 14.68
10 Cocaine 15.45
11 Benzoylecgonine a 16.04
12 Norcocaine d 16.26
13 beta-Truxinic acid b 17.61
14 alpha-Truxillic acid b 17.89
15 cis-Cinnamoylecgonine methyl ester 18.47
16 trans-Cinnamoylecgonine methyl ester 20.55
17 N-Formyl cocaine 22.93
a Chromatographed as the O-TMS derivative.
b Chromatographed as the di-O-TMS derivative.
c Internal standard (ISTD).
d Chromatographed as the N-TMS derivative.
Source: J. F. Casale and R. W. Waggoner, Jr., “A chromatographic impurity signature profile analysis
for cocaine using capillary gas chromatography”, Journal of Forensic Sciences, vol. 36, No. 5 (1991),
pp. 1312-1330. Copyright ASTM International. Reprinted with permission.