E. coli - ForteBio

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Why Aren’t Bound Bacteria Inducing More Signal? • Bacteria are massive compared to the antibodies on the surface on the tip – ~950 bacteria should be able to bind the tip under perfect conditions • Bacteria aren’t all that dense – Mostly water, which light can penetrate easily – E. <strong>coli</strong> are Gram-negative and have a thin cell well • Bacteria are odd shaped – Comparing a cube to a football – Leads to inefficient loading at the tip surface
Simulating Very Dense Particles Goat- Anti- Rabbit Baseline (30 sec.) x56 Rabbit- Anti- Goat Protein G Biosensor Baseline (30 sec.)
Page 1 and 2: Detecting Common Food-borne Pathoge
Page 3 and 4: • Model System Outline • Initia
Page 5 and 6: ELISA Data Demonstrating the Specif
Page 7 and 8: Whole Bacteria: Culture Manipulatio
Page 9: l (nm) 0.45 0.25 0 The Octet Can De
Page 13 and 14: l (nm) Subtracted Data 0.60 0.40 0.
Page 15 and 16: Improving Detection Limit Intact Ba
Page 17 and 18: Protein G Biosensors Disrupted Cell
Page 19 and 20: How to Increase Sensitivity • Dis
Page 21 and 22: Disrupted Cells + Enzymatic Reactio
Page 23 and 24: What Else Can Be Done to Improve Se
Page 25 and 26: Disrupted Cells + Enzymatic Reactio
Page 27 and 28: Bacteria in Hamburger Extract: Cult
Page 29 and 30: l (nm) Bacteria in Hamburger Extrac
Page 31 and 32: Octet vs. Traditional Methods Tradi
Page 33 and 34: Acknowledgements KPL R&D Team
Page 35: Monoclonal Antibodies Polyclonal vs

E. coli - ForteBio

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