E. coli - ForteBio

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Disrupted Cells: Culture Manipulation Grow E. <strong>coli</strong> Assay on Octet RED96 Scrape colonies of the plate and suspend in buffer Pellet for 2 min. Bath Sonicate for 30 min. in EDTA + 1% Triton-X 10-fold Serial Dilution Plate dilutions to determine cfu/ml
Disrupted Cells + Enzymatic Reaction: Detection Streptavidin Biosensors Biotin Streptavidin Biotin Biosensor Biotin Biotin Biotin HRP HRP HRP HRP HRP Antibody Load (300 sec.) Incubate with Metal Enhanced DAB Block w/ Goat Serum (600 sec.) Baseline (30 sec.) Block w/ Goat Serum (300 sec.) Block w/ Goat Serum (300 sec.) Capture Bacteria Particles (300 sec.) HRP
Page 1 and 2: Detecting Common Food-borne Pathoge
Page 3 and 4: • Model System Outline • Initia
Page 5 and 6: ELISA Data Demonstrating the Specif
Page 7 and 8: Whole Bacteria: Culture Manipulatio
Page 9 and 10: l (nm) 0.45 0.25 0 The Octet Can De
Page 11 and 12: Simulating Very Dense Particles Goa
Page 13 and 14: l (nm) Subtracted Data 0.60 0.40 0.
Page 15 and 16: Improving Detection Limit Intact Ba
Page 17 and 18: Protein G Biosensors Disrupted Cell
Page 19: How to Increase Sensitivity • Dis
Page 23 and 24: What Else Can Be Done to Improve Se
Page 25 and 26: Disrupted Cells + Enzymatic Reactio
Page 27 and 28: Bacteria in Hamburger Extract: Cult
Page 29 and 30: l (nm) Bacteria in Hamburger Extrac
Page 31 and 32: Octet vs. Traditional Methods Tradi
Page 33 and 34: Acknowledgements KPL R&D Team
Page 35: Monoclonal Antibodies Polyclonal vs

E. coli - ForteBio

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