E. coli - ForteBio

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Who KPL Is • Founded 1979, KPL developed the first affinity-purified polyclonal antibodies • KPL offers >800 catalog products • Offer kits and applications for protein detection and labeling – ELISA, Western blot, Immunohistology • Polyclonal antibodies – Secondary antibodies & conjugates – Primary antibodies against bacteria
• Model System Outline • Initial Studies with Intact Bacteria • Improving Sensitivity • Improving Signal • Complex Matrices • Future Directions
Page 1: Detecting Common Food-borne Pathoge
Page 5 and 6: ELISA Data Demonstrating the Specif
Page 7 and 8: Whole Bacteria: Culture Manipulatio
Page 9 and 10: l (nm) 0.45 0.25 0 The Octet Can De
Page 11 and 12: Simulating Very Dense Particles Goa
Page 13 and 14: l (nm) Subtracted Data 0.60 0.40 0.
Page 15 and 16: Improving Detection Limit Intact Ba
Page 17 and 18: Protein G Biosensors Disrupted Cell
Page 19 and 20: How to Increase Sensitivity • Dis
Page 21 and 22: Disrupted Cells + Enzymatic Reactio
Page 23 and 24: What Else Can Be Done to Improve Se
Page 25 and 26: Disrupted Cells + Enzymatic Reactio
Page 27 and 28: Bacteria in Hamburger Extract: Cult
Page 29 and 30: l (nm) Bacteria in Hamburger Extrac
Page 31 and 32: Octet vs. Traditional Methods Tradi
Page 33 and 34: Acknowledgements KPL R&D Team
Page 35: Monoclonal Antibodies Polyclonal vs

E. coli - ForteBio

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