Gene Cloning and DNA Analysis: An Introduction, Sixth Edition ...

Recommendations

Info

PART III The Applications of Gene Cloning and DNA Analysis in Biotechnology 13 | Production of Protein from Cloned Genes 225 14 | Gene Cloning and DNA Analysis in Medicine 245 15 | Gene Cloning and DNA Analysis in Agriculture 264 16 | Gene Cloning and DNA Analysis in Forensic Science and Archaeology 282
Page 4:
GENE CLONING AND DNA ANALYSIS
Page 10:
This edition first published 2010,
Page 16:
Contents CONTENTS Preface to the Si
Page 20:
Contents ix 4.3 Ligation—joining
Page 24:
Contents xi 8 How to Obtain a Clone
Page 28:
Contents xiii 11.3 Identifying and
Page 32:
Contents xv 15.1.2 Herbicide resist
Page 36:
PART I The Basic Principles of Gene
Page 42:
4 Part I The Basic Principles of Ge
Page 46:
6 1.4 What is PCR? Figure 1.2 The b
Page 50:
8 Figure 1.3 Cloning allows individ
Page 54:
10 Figure 1.5 Gene isolation by PCR
Page 58:
12 Further reading FURTHER READING
Page 62:
14 Figure 2.1 Plasmids: independent
Page 66:
16 Figure 2.4 Plasmid transfer by c
Page 70:
18 Figure 2.6 Capsid components Pha
Page 74:
20 Figure 2.7 λ phage particle att
Page 78:
22 (a) The linear form of the λ DN
Page 82:
24 Part I The Basic Principles of G
Page 86:
26 Bacterial culture Table 3.1 Cent
Page 90:
28 Bacterial culture Figure 3.3 Har
Page 94:
30 Figure 3.6 Removal of protein co
Page 98:
32 Figure 3.8 Collecting DNA by eth
Page 102:
34 Figure 3.10 DNA purification by
Page 106:
36 Figure 3.12 Two conformations of
Page 110:
38 Figure 3.15 Partial unwinding of
Page 114:
40 Figure 3.18 Preparation of a pha
Page 118:
42 Figure 3.21 Collection of phage
Page 122:
44 Further reading FURTHER READING
Page 126:
Page 130:
48 Figure 4.3 The reactions catalyz
Page 134:
50 Figure 4.6 (a) Alkaline phosphat
Page 138:
52 Figure 4.8 (a) Restriction of ph
Page 142:
54 Figure 4.9 (a) Production of blu
Page 146:
56 Table 4.2 A 10 × buffer suitabl
Page 150:
58 Figure 4.13 Visualizing DNA band
Page 154:
60 Figure 4.15 Using a restriction
Page 158:
62 Figure 4.17 The influence of DNA
Page 162:
64 Figure 4.20 (a) Ligating blunt e
Page 166:
66 Figure 4.23 Adaptors and the pot
Page 170:
68 Figure 4.25 The use of adaptors:
Page 174:
70 Figure 4.28 (a) The ends of the
Page 178:
72 Chapter 5 Introduction of DNA in
Page 182:
Page 186:
76 Figure 5.4 Normal E. coli cell (
Page 190:
78 Figure 5.8 (b) Replica plating W
Page 194:
80 Figure 5.10 (a) The role of the
Page 198:
82 Figure 5.11 (a) A single-strain
Page 202:
84 Figure 5.13 Strategies for the s
Page 206:
86 Figure 5.14 Figure 5.15 (a) Prec
Page 210:
88 Chapter 6 Cloning Vectors for E.
Page 214:
90 during purification. pBR322 is 4
Page 218:
92 (a) pUC8 (b) Restriction sites i
Page 222:
94 Figure 6.5 The M13 genome, showi
Page 226:
96 not totally disrupt the lacZ′
Page 230:
98 Figure 6.10 The λ genetic map,
Page 234:
100 (a) Cloning with a λ replaceme
Page 238:
102 Figure 6.15 (a) A typical cosmi
Page 242:
104 capsid structure. Cosmid-type v
Page 246:
106 Figure 7.1 The yeast 2 µm plas
Page 250:
108 Figure 7.4 Cloning with an E. c
Page 254:
110 Figure 7.7 Chromosome structure
Page 258:
112 for instance to examine the seg
Page 262:
114 Figure 7.10 The Ti plasmid and
Page 266:
116 (a) Wound infection by recombin
Page 270:
118 Figure 7.15 Direct gene transfe
Page 274:
120 Caulimovirus vectors Although o
Page 278:
122 Figure 7.18 Cloning in Drosophi
Page 282:
124 cause the death of the mouse ce
Page 286:
126 Chapter 8 How to Obtain a Clone
Page 290:
128 Figure 8.2 The basic strategies
Page 294:
130 Figure 8.4 (a) Construction of
Page 298:
132 Figure 8.5 Preparation of a gen
Page 302:
134 Figure 8.7 One possible scheme
Page 306:
136 Figure 8.10 The structure of α
Page 310:
138 Figure 8.12 Two methods for the
Page 314:
140 Figure 8.15 A simplified scheme
Page 318:
142 Figure 8.17 Heterologous probin
Page 322:
144 skills (Figure 8.19b). The same
Page 326:
146 The problem of gene expression
Page 330:
148 Figure 9.1 Hybridization of the
Page 334:
150 Figure 9.3 Figure 9.4 5’ 3’
Page 338:
152 Figure 9.7 A typical temperatur
Page 342:
154 Figure 9.9 Calculating the T m
Page 346:
156 Figure 9.13 5’ 3’ A G A C T
Page 350:
158 Figure 9.16 Double-stranded PCR
Page 354:
160 Figure 9.18 Hybridization of a
Page 360:
PART II The Applications of Gene Cl
Page 366:
166 termination sequencing has gain
Page 370:
168 Figure 10.3 Reading the sequenc
Page 374:
170 Figure 10.5 The basis to therma
Page 378:
172 Figure 10.7 Pyrosequencing. Fig
Page 382:
174 Figure 10.9 Part II The Applica
Page 386:
176 Figure 10.11 Using oligonucleot
Page 390:
178 Figure 10.13 Chromosome walking
Page 394:
180 Figure 10.16 Part II The Applic
Page 398:
182 Figure 10.20 Fluorescent in sit
Page 402:
184 s Part II The Applications of G
Page 406:
186 Figure 11.1 Some fundamentals o
Page 410:
188 Figure 11.3 The 5′ end of an
Page 414:
190 Figure 11.6 5' 3' RNA transcrip
Page 418:
192 Figure 11.8 Possible positions
Page 422:
194 Figure 11.11 A bound protein pr
Page 426:
196 Figure 11.14 A modification int
Page 430: 198 Table 11.1 Figure 11.16 A repor
Page 434: 200 Pure mRNA Labelled translation
Page 438: 202 Figure 11.22 (a) Restriction fr
Page 442: 204 Part II The Applications of Gen
Page 450: 208 as molecular biology in silico,
Page 454: 210 Figure 12.4 Part II The Applica
Page 458: 212 Figure 12.7 Using comparisons b
Page 462: 214 Figure 12.10 The use of a yeast
Page 466: 216 Figure 12.11 Serial analysis of
Page 470: 218 Load the protein sample Figure
Page 474: 220 Figure 12.16 Analyzing two prot
Page 486: 226 Figure 13.1 Two different syste
Page 490: 228 Figure 13.3 The three most impo
Page 494: 230 Figure 13.6 Strong and weak pro
Page 498: 232 Part III The Applications of Ge
Page 502: 234 Figure 13.12 Fusion protein bou
Page 506: 236 organism has a bias toward pref
Page 510: 238 Figure 13.16 Part III The Appli
Page 514: 240 Figure 13.18 Crystalline inclus
Page 518: 242 Figure 13.20 Recombinant protei
Page 522: 244 s Part III The Applications of
Page 526: 246 14.1.1 Recombinant insulin Figu
Page 530:
248 Figure 14.2 The synthesis of re
Page 534:
250 Figure 14.5 (a) The factor VIII
Page 538:
252 Figure 14.7 Part III The Applic
Page 542:
Page 546:
256 Part III The Applications of Ge
Page 550:
258 Figure 14.10 Part III The Appli
Page 554:
260 Figure 14.11 Differentiation of
Page 558:
Page 562:
264 Chapter 15 Gene Cloning and DNA
Page 566:
266 Table 15.1 Part III The Applica
Page 570:
268 Figure 15.3 Positional effects.
Page 574:
270 (a) Production of two toxins (c
Page 578:
272 (a) Detoxification of glyphosat
Page 582:
274 are being produced by transferr
Page 586:
276 Figure 15.10 The differences in
Page 590:
278 Figure 15.11 DNA excision by th
Page 594:
Page 598:
282 Chapter 16 Gene Cloning and DNA
Page 602:
284 Figure 16.1 Genetic fingerprint
Page 606:
286 Figure 16.3 Inheritance of STR
Page 610:
Page 614:
290 Figure 16.6 Sex identification
Page 618:
Page 622:
Page 626:
296 Figure 16.11 Origin of agricult
Page 630:
298 Glossary GLOSSARY 2 Fm circle A
Page 634:
300 Glossary Chromosome walking A t
Page 638:
302 Glossary Ethanol precipitation
Page 642:
304 Glossary In vitro mutagenesis A
Page 646:
306 Glossary Nick translation The r
Page 650:
308 Glossary Proteome The entire pr
Page 654:
310 Glossary Single nucleotide poly
Page 658:
312 INDEX Index (g = glossary, f =
Page 662:
314 cloning vectors (continued) exp
Page 666:
316 herpes simplex virus glycoprote
Page 670:
318 polyethylene glycol, see PEG po
Page 674:
320 T m , 153, 305g tobacco, 269 TO
show all

Gene Cloning and DNA Analysis: An Introduction, Sixth Edition ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?