VectorCatalog2012.pdf? - Vector Laboratories

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V E C T O R L A B O R A T O R I E S
VECTASTAIn ® ABC Systems (continued)
Peroxidase
Peroxidase substrates produce sharp, dense precipitates with
crisp localization. These characteristics, in conjuction with the
high sensitivity and low background of the VECTASTAIN ® ABC
systems, make the peroxidase enzyme a preferred choice
VECTASTAIN ® ® ABC System
• Advanced avidin/biotin technology: The ® ABC complex
is smaller, very uniform, and highly active, allowing more
accessibility for binding to a biotinylated target.
• Highest sensitivity, low background: The VECTASTAIN ®
® ABC system is the most sensitive avidin/biotin-based
peroxidase system. The ® ABC series is approximately 5
times more sensitive than the original VECTASTAIN ® ABC Kit
with the same low background.
• Cost effective: Higher sensitivity leads to lower cost per
slide.
• Available without (“Standard” kit) or with biotinylated speciesspecific
or universal secondary antibodies. See page A7.
• Available in Ready-To-Use (R.T.U.) formats: Prediluted,
stabilized working solutions of ® ABC Kit reagents
provide the same high sensitivity and low background as
the traditional VECTASTAIN ® ® ABC Kit reagents.
Original VECTASTAIN ® ABC Kit
• Good sensitivity/low background
• Original avidin/biotin ABC complex formulation
• Low cost
• Available without (“Standard” kit) or with biotinylated
species-specific secondary antibody. See page A8.
Tonsil: • Multi-Cytokeratin (m), VECTASTAIN ® Elite® ABC Kit, Vector ®
DAB (brown) • CD3 (m), VECTASTAIN ® Elite® ABC Kit, Vector ® VIP
(purple) • CD20 (m), VECTASTAIN ® Elite® ABC Kit, Vector ® SG (bluegray).
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in many applications. (eg. In neural tissue, the peroxidase
system is often preferred because it gives more consistent
labeling of both cell bodies and processes.) For peroxidase
substrates see pages A18-A21.
VECTASTAIN ® Universal Kits
The VECTASTAIN ® Universal Kit is designed for rapid
immunohistochemical staining of tissue sections. The kit relies
on a proprietary preformed peroxidase-streptavidin complex to
achieve outstanding sensitivity with short incubation times.
• Rapid protocol: Staining in less than 20 minutes following
primary antibody incubation. Working solutions can be used
immediately after dilution.
• High sensitivity, low background.
• Biotinylated, Universal Pan-Specific secondary antibody
recognizes mouse, rabbit, and goat primary antibodies as
well as those from related species such as rat, bovine, and
sheep.*
• Available in Ready-To-Use (R.T.U) format: Prediluted,
stabilized working solutions of VECTASTAIN ® Universal
Kit reagents provide the same high sensitivity and low
background as the concentrated VECTASTAIN ® Universal
Kit. See page A8.
* Do not use the pan-specific secondary antibody to stain rat, mouse or other
rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with
endogenous IgG.
Prostate: Desmin (m), VECTASTAIN ® Universal Quick Kit, ImmPACT
DAB (brown) substrate. Hematoxylin QS (blue) counterstain.