VectorCatalog2012.pdf? - Vector Laboratories

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V E C T O R L A B O R A T O R I E S
Accessory Reagents
Control Antibodies
These IgG preparations (I-1000, I-2000, I-4000, and I-5000)
are intended for use as controls for primary antibodies
made in rabbit, mouse, rat, or goat. Supplied as lyophilized
powders, these antibodies have been purified from pooled
serum of healthy adult animals and contain a spectrum of the
IgG subclasses present in serum. They should be applied to
the control tissue section at the same concentrations as the
primary antibody. These control sections will help determine
whether staining with the primary antibody is specific for
the antigen or whether staining is the result of nonspecific
adsorption of primary antibody to tissue sites.
VECTABOND Reagent
VECTABOND Reagent (SP-1800) is designed to significantly
enhance the adherence of both frozen and paraffinembedded
tissue sections and cell preparations to glass slides
and coverslips. Tissue sections will remain attached even
when subjected to the most extreme conditions, such as
high temperature antigen unmasking techniques and in situ
hybridization applications. VECTABOND Reagent-treated slides
can be stored for long periods. This product chemically modifies
the glass to form a highly adherent surface. VECTABOND
Reagent is provided as a 7 ml concentrate that dilutes to 350 ml
of treatment solution, sufficient for at least 500 standard slides.
ImmEdge Hydrophobic Barrier Pen
The ImmEdge Pen (H-4000) is a hydrophobic barrier pen for
immunohistochemistry and in situ hybridization. It is designed
to provide a heat-stable, water-repellent barrier that keeps
reagents localized on tissue specimens and prevents mixing
of reagents when multiple sections are mounted on the same
slide.
The pale blue barrier is insoluble in alcohol and acetone but is
completely removed by all commonly used xylene and “xylene
substitute” clearing agents. The ImmEdge Pen contains no
ozone-depleting solvents and is compatible with enzyme- or
fluorescence-based detection systems.
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ImmPrint Histology Pen
The ImmPrint Histology Pen (H-6100) is a solvent-resistant
permanent marking pen, designed for writing on glass
microscope slides, tissue cassettes, and most hard surfaces.
The high density, fast-drying black ink is resistant to most
organic solvents encountered in histological applications, such
as alcohols or clearing agents. Unlike other pens commonly
used for histology, the ImmPrint pen has a smooth writing
tip that resists drying out.
Antigen Unmasking Solutions
Vector Laboratories’ Antigen Unmasking Solutions are highly
effective at revealing antigens in formalin-fixed, paraffinembedded
tissue sections when used in combination with
a high temperature treatment procedure. The Antigen
Unmasking Solutions are available in two formulations. The
citrate-based solution (H-3300) is pH 6.0; the Tris-based
solution (H-3301) is pH 9.0. Both solutions are supplied as
convenient 100x concentrated stocks, sufficient for preparation
of 25 L of working solution.
Accessory Reagents
Product
Control Antibodies
Catalog
Number Unit Size
Rabbit IgG I-1000 5 mg
Mouse IgG I-2000 1 mg
Rat IgG I-4000 1 mg
Goat IgG I-5000 5 mg
VECTABOND Reagent SP-1800 7 ml
ImmEdge Pen H-4000 2 pens
ImmPrint Histology Pen H-6100 5 pens
Antigen Unmasking Solution,
Citrate-based (100x)
Antigen Unmasking Solution, Trisbased
(100x)
H-3300 250 ml
H-3301 250 ml