VectorCatalog2012.pdf? - Vector Laboratories

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V E C T O R L A B O R A T O R I E S
Blocking Reagents
BLOXALL Endogenous Peroxidase/Alkaline
Phosphatase Blocking Solution
• Inhibits endogenous peroxidase, pseudoperoxidase, and
alkaline phosphatase activities
• Ready-to-use in convenient dropper bottle
• Simple one-step protocol
• Compatible with formalin-fixed, paraffin-embedded tissue
sections, frozen tissue sections, and cell preparations
• More effective than conventional blocking methods
Tissues can contain endogenous peroxidase, pseudoperoxidase,
and/or alkaline phosphatase activity that will produce
background staining if an alkaline phosphatase and/or
peroxidase detection system and corresponding substrates are
used. BLOXALL Endogenous Peroxidase/Alkaline Phosphatase
Blocking Solution (SP-6000) inactivates endogenous peroxidase,
pseudoperoxidase, and alkaline phosphatase in formalin-fixed,
paraffin-embedded tissue sections, frozen tissue sections, and
cell preparations in a single 10 minute incubation step. This
reagent is provided as a ready-to-use reagent in a convenient
dropper bottle.
Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in
frozen, acetone-fixed intestine revealed with Vector ® Red AP Substrate
(magenta) and ImmPACT DAB HRP Substrate (brown), (left). Same
substrates used on BLOXALL Solution-treated tissue (right). BLOXALL
Blocking Solution completely eliminates both endogenous enzyme
activities.
w w w . V E C T O R L A B S . C O m
Levamisole Solution
Tissues can contain endogenous alkaline phosphatase activity
that will produce background staining, if an alkaline phosphatase
detection system and substrate are used. To eliminate this
problem, Levamisole Solution (SP-5000), an inhibitor of alkaline
phosphatase, is provided in a convenient dropper bottle, and
can be added to the alkaline phosphatase substrate solution.
Levamisole does not inhibit the isoenzyme used for either the
VECTASTAIN ® ABC-AP reagents or other alkaline phosphatase
conjugates. 100x concentrate.
Avidin/Biotin Blocking Kit
Avidin/Biotin Blocking Kit (SP-2001) blocks all endogenous
biotin, biotin receptors, and avidin binding sites present
in tissues. This kit is designed for use with biotin/avidin
detection systems such as the VECTASTAIN ® ABC Kits if avidin
or biotinylated reagents bind non-specifically to tissues or
proteins. This blocking kit consists of 18 ml of Avidin D and 18 ml
of biotin in convenient dropper bottles.
Streptavidin/Biotin Blocking Kit
Streptavidin/Biotin Blocking Kit (SP-2002) blocks all
endogenous biotin, biotin receptors, and streptavidin binding
sites present in tissues. This kit is designed for use with biotin/
streptavidin detection systems such as the VECTASTAIN ®
Universal kits if streptavidin or biotinylated reagents bind
non-specifically to tissues or proteins. This blocking kit consists
of 18 ml of streptavidin and 18 ml of biotin in convenient
dropper bottles.