VectorCatalog2012.pdf? - Vector Laboratories
VectorCatalog2012.pdf? - Vector Laboratories
VectorCatalog2012.pdf? - Vector Laboratories
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A<br />
A<br />
22<br />
V E C T O R L A B O R A T O R I E S<br />
multiple Antigen Labeling<br />
<strong>Vector</strong> <strong>Laboratories</strong> is a leader in multiple antigen labeling<br />
applications because of our wide range of very sensitive and<br />
very low background detection reagents, our proprietary<br />
enzyme substrates, and our rigorously tested protocols. These<br />
detection systems and unique enzyme substrates offer an<br />
extensive choice of color combinations for multiple labeling<br />
using either:<br />
• The same enzyme system with different substrates to detect<br />
each antigen, or<br />
• Different enzyme systems and their substrates to detect each<br />
antigen<br />
Enzyme Substrate Combinations<br />
w w w . V E C T O R L A B S . C O m<br />
The photos on the following page illustrate a few possible<br />
combinations that give excellent results. The order of labeling,<br />
use of control sections, and additional blocking steps may be<br />
important to obtain optimal results. For a detailed description<br />
of these applications, protocols, and additional images, please<br />
visit our website or request a free copy of our brochure,<br />
“Discovery Through Color: A Guide to Multiple Antigen<br />
Labeling”.<br />
This table shows the substrates that are recommended for multiple antigen labeling and the order in which they are optimally used.<br />
Second Substrate<br />
First Substrate<br />
<strong>Vector</strong> ® Red<br />
(magenta)<br />
SK-5100<br />
<strong>Vector</strong> ® Blue<br />
(blue)<br />
SK-5300<br />
BCIP/NBT<br />
(indigo)<br />
SK-5400<br />
VIP/<br />
ImmPACT <br />
VIP<br />
(purple)<br />
SK-4605,<br />
SK-4600<br />
DAB/<br />
ImmPACT <br />
DAB<br />
(brown)<br />
SK-4105,<br />
SK-4100<br />
DAB-Ni<br />
(gray-black)<br />
SK-4100<br />
NovaRED /<br />
ImmPACT <br />
NovaRED <br />
(red)<br />
SK-4805,<br />
SK-4800<br />
SG/<br />
ImmPACT AEC/<br />
SG ImmPACT<br />
(blue-gray)<br />
SK-4705,<br />
SK-4700<br />
AEC<br />
(red)<br />
SK-4205,<br />
SK-4200<br />
<strong>Vector</strong> ® Red (magenta)<br />
Cat. No. SK-5100 – – – + + – + – –<br />
<strong>Vector</strong> ® Blue (blue)<br />
Cat. No. SK-5300 + – + + + + + + +<br />
BCIP/NBT (indigo)<br />
Cat. No. SK-5400 + – + + + + + + +<br />
VIP/ImmPACT VIP<br />
(purple)<br />
Cat. No. SK-4605, SK-4600<br />
DAB/ImmPACT DAB<br />
(brown)<br />
Cat. No. SK-4105, SK-4100<br />
ImmPACT <br />
– + – + + – + – –<br />
AMEC Red<br />
(red)<br />
SK-4285<br />
+ + + + – – + + +<br />
DAB-Ni (gray-black)<br />
Cat. No. SK-4100 + – – + + + – – –<br />
NovaRED /ImmPACT <br />
NovaRED (red)<br />
Cat. No. SK-4805, SK-4800<br />
SG/ImmPACT SG<br />
(blue-gray)<br />
Cat. No. SK-4705, SK-4700<br />
AEC/ImmPACT AEC<br />
(red)<br />
Cat. No. SK-4205, SK-4200<br />
ImmPACT AMEC Red<br />
(red)<br />
SK-4285<br />
– + + – + + + – –<br />
+ – – + + – – + +<br />
– – – – + – – + –<br />
– – – – + – – + –<br />
Key: Alkaline Phosphatase Peroxidase + Indicates good contrast – Indicates incompatiblity of substrates for various reasons
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