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VectorCatalog2012.pdf? - Vector Laboratories

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A<br />

A<br />

10<br />

V E C T O R L A B O R A T O R I E S<br />

VECTASTAIn ® ABC Systems (continued)<br />

Glucose Oxidase<br />

Endogenous glucose oxidase activity does not exist in<br />

mammalian tissues, so the VECTASTAIN ® ABC-GO Kit can be<br />

used when endogenous peroxidases or alkaline phosphatases<br />

interfere with specific staining and blocking of that<br />

VECTASTAIN ® ABC-GO Kits (Glucose Oxidase)<br />

Product<br />

VECTASTAIN ® ABC-GO Kit,<br />

Standard<br />

VECTASTAIN ® ABC-GO Kit,<br />

Rabbit IgG<br />

VECTASTAIN ® ABC-GO Kit,<br />

Mouse IgG<br />

Catalog<br />

Number<br />

Unit<br />

Size ABC Reagent<br />

OK-3000 1 Kit 2 ml Reagent A<br />

2 ml Reagent B<br />

(200 ml working<br />

solution)<br />

OK-3001 1 Kit 2 ml Reagent A<br />

2 ml Reagent B<br />

(200 ml working<br />

solution)<br />

OK-3002 1 Kit 2 ml Reagent A<br />

2 ml Reagent B<br />

(200 ml working<br />

solution)<br />

w w w . V E C T O R L A B S . C O m<br />

endogenous enzyme activity is ineffective or would destroy<br />

antigenic determinants. The VECTASTAIN ® ABC Glucose<br />

Oxidase Kit is the least sensitive of the VECTASTAIN ® ABC<br />

systems. For glucose oxidase substrates see pages A18-A21.<br />

Secondary<br />

Antibody<br />

Normal Blocking<br />

Serum<br />

none none 1000-2000<br />

1 ml biotinylated<br />

goat anti-rabbit IgG<br />

(200 ml working<br />

solution)<br />

1 ml biotinylated<br />

horse anti-mouse IgG<br />

(200 ml working<br />

solution)<br />

3 ml normal goat<br />

serum<br />

(200 ml working<br />

solution)<br />

3 ml normal horse<br />

serum<br />

(200 ml working<br />

solution)<br />

Approximate Number<br />

of sections stained<br />

1000-2000<br />

1000-2000

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