VectorCatalog2012.pdf? - Vector Laboratories

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V E C T O R L A B O R A T O R I E S
mouse on mouse (m.O.m. ) Immunodectection kits
The Vector ® M.O.M. Immunodetection Kits are specifically
designed to localize mouse primary antibodies on mouse
tissues. The major problem with using mouse primary
antibodies on mouse tissues in immunohistochemistry is the
inability of the anti-mouse secondary antibody to distinguish
between the mouse primary antibody and endogenous
mouse immunoglobulins in the tissue. This can result in high
background staining which obscures specific staining. This
problem can be essentially eliminated by using a Vector ®
M.O.M. Immunodetection Kit which includes a proprietary
M.O.M. Mouse Ig Blocking Reagent with a specialized
M.O.M. Biotinylated Anti-Mouse Ig to significantly reduce
undesired binding of the secondary antibody to endogenous
tissue immunoglobulin.
Key advantages of the Vector ® M.O.M.
Immunodetection Kits:
• Significant reduction of endogenous mouse Ig staining when
using mouse primary antibodies on mouse tissue
• Clear, crisp specific staining of antigens of interest
• Based on VECTASTAIN ® ABC reagent protocols
• Procedures are simple and easy to follow
• No tedious calculations or antibody prebinding steps
required
The Vector ® M.O.M. Immunodetection Kits are useful in any
mouse-on-mouse application, such as studies in genetically
engineered mice, including transgenic and knock-out models,
as well as mouse xenografts.
The M.O.M. Kits can be also used in multiple antigen staining
protocols to localize two different mouse primary antibodies on
the same mouse tissue sections using fluorescent or enzymebased
detection systems. Detailed information for multiple
antigen labeling can be found in our brochure, “ Discovery
Through Color: A Guide to Multiple Antigen Labeling”,
available on our website.
Three Vector ® M.O.M. Kits are available. All kits use the
same proprietary M.O.M. Mouse Ig Blocking Reagent and
the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a
choice of an enzyme-based or fluorescent-based visualization
method. M.O.M. Mouse Ig Blocking Reagent and M.O.M.
Biotinylated Anti-Mouse Ig Reagent are also available
separately.
w w w . V E C T O R L A B S . C O m
Vector ® M.O.M. Peroxidase Kit
Vector ® M.O.M. Peroxidase Kit (PK-2200) includes the
following:
• M.O.M. Mouse Ig Blocking Reagent
• M.O.M. Biotinylated Anti-Mouse Ig Reagent
• M.O.M. Protein Concentrate for general protein blocking
and diluent
• VECTASTAIN ® ® ABC Reagent
Choose from the wide variety of peroxidase substrates
available (pages A18-A21) for chromogenic development.
Mouse Newborn Tongue: • Synapsin (m), M.O.M. Peroxidase Kit,
Vector ® NovaRED (red) • Desmin (m), M.O.M. Peroxidase Kit,
Vector ® DAB-Ni (black).
Vector ® M.O.M. Fluorescein Kit
Vector ® M.O.M. Fluorescein Kit (FMK-2201) includes the
following:
• M.O.M. Mouse Ig Blocking Reagent
• M.O.M. Biotinylated Anti-Mouse Ig Reagent
• M.O.M. Protein Concentrate for general protein blocking
and diluent
• Fluorescein Avidin DCS (green fluorescence, excitation at
495 nm; emission at 515 nm)
Mouse Intestine: • Desmin (m), M.O.M. Fluorescein Kit (green)
• Multi-Cytokeratin (m), M.O.M. Basic Kit, Texas Red ® Avidin DCS.