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VectorCatalog2012.pdf? - Vector Laboratories

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A<br />

A<br />

14<br />

V E C T O R L A B O R A T O R I E S<br />

ImmPRESS Polymer kits (continued)<br />

Multiple Antigen Labeling Simplified<br />

The ready-to-use, one-step, non-biotin, sensitive ImmPRESS <br />

Reagent significantly shortens staining times for multiple<br />

antigen labeling. The reduced number of steps compared<br />

to a conventional protocol directly decreases the amount of<br />

slide handling. In systems containing endogenous biotin, the<br />

ImmPRESS Reagent eliminates the need for avidin/biotin<br />

blocking steps. Detailed information for multiple antigen<br />

labeling can be found in our brochure, “ Discovery Through<br />

Color: A Guide to Multiple Antigen Labeling”, that is available<br />

on our website.<br />

Breast Carcinoma: • Ki67 (rm), ImmPRESS Reagent (HRP;<br />

Universal), <strong>Vector</strong> ® DAB (brown) • CD34, ImmPRESS Reagent<br />

(HRP; Universal), <strong>Vector</strong> ® VIP (purple).<br />

w w w . V E C T O R L A B S . C O m<br />

Colon: • M2A antigen (m), VECTASTAIN ® ABC-AP Kit (Universal),<br />

<strong>Vector</strong> ® Blue (blue) • CD20 (m), ImmPRESS Reagent (HRP;<br />

Universal), <strong>Vector</strong> ® VIP (purple).<br />

Colon: • CD3 (rm), ImmPRESS Reagent (HRP) Anti-Rabbit Ig,<br />

ImmPACT AMEC Red • CD34 (m), <strong>Vector</strong> ® AP detection system,<br />

<strong>Vector</strong> ® Blue (blue).<br />

Add First Primary Antibody Add ImmPRESS Reagent Add Enzyme Substrate I<br />

Add Second Primary Antibody Add ImmPRESS Reagent Add Enzyme Substrate II

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