VectorCatalog2012.pdf? - Vector Laboratories

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A 28 V E C T O R L A B O R A T O R I E S Enzyme Conjugated Secondary Antibodies A Our high affinity antibodies are purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. These antibodies are then cross-linked with the highest specific activity alkaline phosphatase or horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. These antibodies are subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots. For maximum sensitivity and convenience, see also the one-step ImmPRESS Peroxidase Polymer Reagents (pages A12-A15). Enzyme Conjugated Secondary Antibodies Product Alkaline Phosphatase Anti-Mouse IgG (H+L) made in horse Alkaline Phosphatase labeled Anti-Rabbit IgG (H+L) made in goat Alkaline Phosphatase labeled Anti-Human IgG (H+L) made in goat Alkaline Phosphatase labeled Anti-Goat IgG H+L) made in horse Alkaline Phosphatase labeled Peroxidase Anti-Mouse IgG (H+L) made in horse Peroxidase labeled Anti-Rabbit IgG (H+L) made in goat Peroxidase labeled Anti-Human IgG (H+L) made in goat Peroxidase labeled Anti-Goat IgG (H+L) made in horse Peroxidase labeled Catalog Number Unit Size AP-2000 1.0 ml AP-1000 1.0 ml AP-3000 1.0 ml AP-9500 1.0 ml PI-2000 1.0 mg PI-1000 1.0 mg PI-3000 1.0 mg PI-9500 1.0 mg w w w . V E C T O R L A B S . C O m Avidin and Streptavidin Enzyme Conjugates Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules. Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates. These enzyme conjugates are suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications. Enzyme Conjugated Avidin and Streptavidin Product Alkaline Phosphatase Catalog Number Unit Size Alkaline Phosphatase Streptavidin SA-5100 1 ml Alkaline Phosphatase Avidin Da Peroxidase A-2100 100 U Horseradish Peroxidase Streptavidin, concentrate Horseradish Peroxidase Streptavidin, R.T.U. Horseradish Peroxidase Avidin D, concentrate SA-5004 1 mg SA-5704 100 ml A-2004 5 mg Horseradish Peroxidase Avidin D, R.T.U. A-2704 100 ml Beta-Galactosidase Beta-Galactosidase Avidin Db Glucose Oxidase A-2300 100 U Glucose Oxidase Avidin D A-2200 5 mg a One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 ºC, pH 9.8. b One unit is 1 µmole o-nitrophenyl-ß-D-galactopyranoside hydrolyzed per min. at 37 ºC, pH 7.3.
Biotinylated Secondary Antibodies Vector Laboratories’ affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the Product Anti-Cat IgG (H+L) biotinylated, made in goat Anti-Chicken IgG (H+L) biotinylated, made in goat Anti-Goat IgG (H+L) a, d biotinylated, made in rabbit Anti-Goat IgG (H+L) a biotinylated, made in horse Anti-Guinea Pig IgG (H+L) d biotinylated, made in goat Anti-Hamster IgG (H+L) biotinylated, made in goat Anti-Horse IgG (H+L) biotinylated, made in goat Anti-Human IgG (H+L) d biotinylated, made in goat Anti-Human IgA, biotinylated, α chain specific, made in goat Anti-Human IgE, biotinylated, ε chain specific, made in goat Anti-Human IgG, biotinylated, γ chain specific, made in goat Anti-Human IgM, biotinylated, µ chain specific, made in goat Anti-Human Kappa Chain biotinylated, κ chain specific, made in goat Anti-Human Lambda Chain biotinylated, λ chain specific, made in goat Catalog Number Unit Size BA-9000 1.5 mg BA-9010 1.5 mg BA-5000 1.5 mg BA-9500 1.5 mg BA-7000 1.5 mg BA-9100 1.5 mg BA-8000 1.5 mg BA-3000 1.5 mg BA-3030 0.5 mg BA-3040 0.5 mg BA-3080 0.5 mg BA-3020 0.5 mg BA-3060 0.5 mg BA-3070 0.5 mg a - Suitable for use with goat, sheep, and bovine IgG primary antibodies. b - Designed for use in rat tissues. c - Designed for use in mouse tissues. d - Antibodies included in VECTASTAIN ® ABC Kits. T O O R d E R C A L L ( 8 0 0 ) 2 2 7 - 6 6 6 6 ImmunOhISTOChEmISTRy A29 antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots. Most of these antibodies are supplied in lyophilized form and can be reconstituted with 1 ml water. With some exceptions the recommended dilution for most applications is 1:200. (H+L) indicates the antibody recognizes both heavy and light chains. Product Anti-Mouse IgG (H+L) d biotinylated, made in horse Anti-Mouse IgG (H+L) b biotinylated, rat adsorbed, made in horse Anti-Mouse IgG (H+L) biotinylated, made in goat Anti-Mouse IgM d , biotinylated, μ chain specific, made in goat Anti-Rabbit IgG (H+L) d biotinylated, made in goat Anti-Rabbit IgG (H+L) biotinylated, made in horse Anti-Rat IgG (H+L) d biotinylated, made in rabbit Anti-Rat IgG (H+L) c , biotinylated, mouse adsorbed, made in rabbit Anti-Rat IgG (H+L) biotinylated, made in goat Anti-Rat IgG (H+L) c , biotinylated, mouse adsorbed, made in goat Anti-Sheep IgG (H+L) a, d biotinylated, made in rabbit Anti-Swine IgG (H+L) biotinylated, made in goat Universal Anti-Mouse/Rabbit IgG (H+L) d, e biotinylated, made in horse Universal Pan-Specific Anti-Mouse/ Rabbit/Goat IgG (H+L) f, g biotinylated, made in horse Catalog Number Unit Size BA-2000 1.5 mg BA-2001 0.5 mg BA-9200 1.5 mg BA-2020 0.5 mg BA-1000 1.5 mg BA-1100 1.5 mg BA-4000 1.5 mg BA-4001 0.5 mg BA-9400 1.5 mg BA-9401 0.5 mg BA-6000 1.5 mg BA-9020 1.5 mg BA-1400 2.1 mg BA-1300 2.2 ml e - Universal Anti-Mouse/Rabbit IgG (BA-1400) should be reconstituted with 2 ml water and diluted 1:50 for use. Do not use the Universal antibody to stain rodent or rabbit tissue because of cross reactivity with endogenous IgG. f - Universal Pan-Specific Anti-Mouse/Rabbit/Goat IgG (BA-1300) should be diluted 1:20. Do not use this Pan-Specific antibody to stain rodent, rabbit, goat, sheep, or bovine tissue because of cross reactivity with endogenous IgG. g - Antibody used in the VECTASTAIN ® Universal Quick Kits. A
Page 1 and 2: VECTOR L A B O R A T O R I E S
Page 3 and 4: QuICk guIdE Immunohistochemistry VE
Page 5 and 6: T O O R d E R C A L L ( 8 0 0 ) 2 2
Page 7 and 8: Proprietary Enzyme Substrates. Alon
Page 9 and 10: Choosing a VECTASTAIN ® ABC Kit 1)
Page 11 and 12: VECTASTAIN ® ABC ® Kits (Peroxida
Page 13 and 14: Alkaline Phosphatase The sensitivit
Page 15 and 16: VECTASTAIN ® ABC Custom Kits If a
Page 17 and 18: ImmPRESS Kits Product Catalog Numb
Page 19 and 20: Breast Carcinoma: • Estrogen Rece
Page 21 and 22: Vector ® M.O.M. Basic Kit Vector
Page 23 and 24: Enzyme Substrate Properties Substra
Page 25 and 26: Enzyme Substrate Kits Enzyme Substr
Page 27 and 28: Multiple Label Slides Tonsil: • C
Page 29 and 30: Counterstain/Substrate Compatibilit
Page 31: Normal Sera Our Normal Sera (S-1000
Page 35 and 36: Vectamount mounting media Permanen
Page 39 and 40: Choice of Fluorophores. We offer se
Page 41 and 42: Antibody Description Conjugate Colo
Page 43 and 44: Anti-Streptavidin and Anti-Avidin A
Page 45 and 46: VECTAShIELd ® mounting media for f
Page 47 and 48: Blocking Reagents Normal Sera Our N
Page 51 and 52: Detection and Amplification. Factor
Page 53 and 54: Fluorochrome-Labeled Streptavidin a
Page 55 and 56: Detection of Fluorescein-Labeled Pr
Page 57 and 58: VECTASHIELD ® Mounting Media for F
Page 59 and 60: Alkaline Phosphatase Detection Opti
Page 61 and 62: Alkaline Phosphatase Detection Opti
Page 63 and 64: Detection of DNP- or Texas Red ® -
Page 65 and 66: Permanent Mounting Medium VectaMoun
Page 67 and 68: ImmEdge Hydrophobic Barrier Pen Th
Page 71 and 72: neuronal Tracers Anterograde/Retrog
Page 73 and 74: neuronal glycobiology Reagents T O
Page 77 and 78: Reagent Options for Enzymatic Detec
Page 79 and 80: Amplified Detection Greater sensiti
Page 81 and 82: Detection of Fusion Protein Tags Fu
Page 83 and 84:
UltraSNAP Detection System for Sou
Page 85 and 86:
Blocking Reagents 10x Casein Soluti
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Sequence Specific dnA Ligands Resol
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T O O R d E R C A L L ( 8 0 0 ) 2 2
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Some commonly used labels that are
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FastTag ® and PHOTOPROBE ® Reagen
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EndTag Labeling Kits Product Catal
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Thiol Group Modification (continued
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Biotin Quantitation kit Quant*Tag
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T O O R d E R C A L L ( 8 0 0 ) 2 2
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Amyloid Precursor Protein Clone 3G1
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CD1a Clone JPM30 VP-C311 • 1 ml H
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CD23 Clone 1B12 VP-C339 • 1 ml H,
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CD66a (CEACAM1) Clone 29H2 VP-C363
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c-kit Oncoprotein (CD117) Clone T59
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Cytokeratin 10 Clone LHP1 VP-C408
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Dystrophin Rod Domain Clone Dy4/6D3
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Galectin-3 Clone 9C4 VP-G802 • 1
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Ki67 is a cell cycle associated pro
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Myeloperoxidase Clone 59A5 VP-M661
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p73 Protein Clone 24 VP-P961 • 1
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to its reported high expression in
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Tyrosinase Clone T311 VP-T488 • 1
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Product Conjugate Catalog Number Un
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Anti-Avidin and Anti-Streptavidin A
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Anti-Fluorescein Fluorescein is not
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BIOTIn And AVIdIn/STREPTAVIdIn REAg
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Biotinylated Lectins These conjugat
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Biotinylated Antibodies against Fus
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Avidin and Streptavidin Reagents Av
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Anti-Avidin and Anti-Streptavidin R
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VECTREX ® Matrices for Nucleic Aci
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T O O R d E R C A L L ( 8 0 0 ) 2 2
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Substrates at a glance Properties S
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Enzyme Substrate kits Peroxidase En
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Vector ® NovaRED Substrates Vecto
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Endogenous Peroxidase Blocking BLOX
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BCIP/NBT Substrate Kit 5-bromo-4-ch
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Applications The DuoLuX Chemilumin
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T O O R d E R C A L L ( 8 0 0 ) 2 2
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Agarose Bound Lectins Lectin affini
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Chitin Hydrolysate Chitin Hydrolysa
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VECTREX ® matrices for nucleic Aci
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T O O R d E R C A L L ( 8 0 0 ) 2 2
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Agarose Bound Lectins Lectin affini
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Lectin Mitogenic Activity Blood Gro
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Lectin Mitogenic Activity Blood Gro
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Datura Stramonium Lectin (DSL) DSL
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Jacalin Jacalin is a lectin compose
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Phaseolus Vulgaris Agglutinin (PHA)
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Solanum Tuberosum (Potato) Lectin (
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Lectin Screening kits Our lectin sc
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Antibodies to Lectins These antibod
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T O O R d E R C A L L ( 8 0 0 ) 2 2
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PAGE PRODUCT DESCRIPTION NO. Animal
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PAGE PRODUCT DESCRIPTION NO. Avidin
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PAGE PRODUCT DESCRIPTION NO. Casein
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PAGE PRODUCT DESCRIPTION NO. Fluore
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PAGE PRODUCT DESCRIPTION NO. Lens C
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PAGE PRODUCT DESCRIPTION NO. PHA-E+
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PAGE PRODUCT DESCRIPTION NO. Strept
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Catalog number Index CATALOG PAGE N
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CATALOG PAGE NO. NO. MB-8000 F4-F5
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CATALOG PAGE NO. NO. VP-M655 G20 VP
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histology flow cytometry fusion pro
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