VectorCatalog2012.pdf? - Vector Laboratories

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V E C T O R L A B O R A T O R I E S
fluorochrome-Labeled Secondary Antibodies
Our affinity purified antibodies are of unmatched quality for
use in immunological techniques. All antibodies are prepared
using proprietary immunization schedules that produce
high affinity antibodies. The antibodies are then purified
by affinity chromatography, and cross-reactivities that are
likely to interfere with specific labeling are removed by solid
phase adsorption techniques. Antibodies are conjugated in a
manner that ensures the maximum degree of labeling without
compromising the specificity or affinity of the antibody.
Vector Laboratories produces fluorochrome-labeled secondary
antibodies conjugated using traditional fluors – fluorescein,
rhodamine, Texas Red ® , AMCA, phycoerythrin – as well as the
newer DyLight ® dyes.
Astrocytes: GFAP (rp), DyLight ® 488 anti-rabbit IgG, mounted in
VECTASHIELD ® HardSet Mounting Medium with DAPI. (Image
courtesy of Dr. Emma East, Department of Life Sciences, The Open
University, U.K.)
Secondary Antibodies
Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).
Antibody Description Conjugate Color (Ex/Em)
Mouse
Anti-Mouse IgG (H+L), made in horse
Anti-Mouse IgG Kit, made in horse
- 500 μg AMCA Anti-Mouse IgG
- 500 μg Fluorescein Anti-Mouse IgG
- 500 μg Texas Red ® Anti-Mouse IgG
Anti-Mouse IgG (H+L), rat adsorbed,
made in horse
Anti-Mouse IgM, mu chain specific,
made in goat
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DyLight ® dyes offer several advantages including greater
photostability, pH independence and brighter fluorescence.
Vector Laboratories offers DyLight ® conjugated antimouse
IgG and anti-rabbit IgG antibodies for use in a
variety of applications, in particular, cell- and tissue-based
immunofluorescent antigen detection/visualization. The
DyLight ® conjugates are completely stable from pH 4 to pH 9,
making them compatible with many buffers and diluents.
Phycoerythrin is a fluorescent protein that produces a bright
red-orange fluorescence. Our newly improved Phycoerythrin
conjugates are substantially brighter than previous conjugates.
Phycoerythrin-conjugated antibodies can be used in cell sorting
and in tissue staining.
Catalog
Number
Unit
Size
Stock
Concentration
AMCA Blue (350/450) CI-2000 1.5 mg 1.5 mg/ml 1:150
DyLight ® 488 Green (493/518) DI-2488 1.5 mg 1.5 mg/ml 1:150
Fluorescein Green (495/515) FI-2000 1.5 mg 1.5 mg/ml 1:150
DyLight ® 549 Orange (556/571) DI-2549 1.5 mg 1.5 mg/ml 1:150
R-Phycoerythrin Red-Orange (565/574) EI-2007 1.0 mg 1.0 mg/ml 1:100
DyLight ® 594 Red (592/617) DI-2594 1.5 mg 1.5 mg/ml 1:150
Texas Red ® Red (595/615) TI-2000 1.5 mg 1.5 mg/ml 1:150
DyLight ® 649 Far Red (655/670) DI-2649 1.5 mg 1.5 mg/ml 1:150
AMCA,
Fluorescein,
Texas Red ®
Blue (350/450)
Green (495/515)
Red (595/615)
Astrocytes: GFAP (rp), DyLight ® 594 anti-rabbit IgG, mounted in
VECTASHIELD ® HardSet Mounting Medium with DAPI. (Image
courtesy of Dr. Emma East, Department of Life Sciences, The Open
University, U.K.)
FI-2100 1 kit 0.5 mg/ml
0.5 mg/ml
0.5 mg/ml
Recommended
Dilution*
1:50
1:50
1:50
Fluorescein Green (495/515) FI-2001 0.5 mg 1.0 mg/ml 1:50
AMCA Blue (350/450) CI-2020 0.5 mg 0.5 mg/ml 1:25
Fluorescein Green (495/515) FI-2020 0.5 mg 1.0 mg/ml 1:100
Texas Red ® Red (595/615) TI-2020 0.5 mg 1.0 mg/ml 1:100
* The dilution factors shown are recommended for most applications.