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Microbiology and Spoilage Trail in Nile Perch (Lates niloticus), Lake ...

Microbiology and Spoilage Trail in Nile Perch (Lates niloticus), Lake ...

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49<br />

Figure 13: Changes of specific spoilage<br />

organisms (SSO), total viable counts<br />

(TVC), Enterobacteriaceae (Ent.) <strong>and</strong><br />

TVB-N <strong>in</strong> chilled fillets from newly<br />

caught <strong>Nile</strong> perch.<br />

4.3 PHASE III: MICROBIOLOGY OF WHOLE NILE PERCH SWABS, ON LINE<br />

FILLETS AND SHELF LIFE OF ESTABLISHMENTS CHILLED FILLETS<br />

4.3.1 Phase III - Trial 1: <strong>Microbiology</strong> of whole <strong>Nile</strong> perch swabs <strong>and</strong> onl<strong>in</strong>e fillets<br />

Whole <strong>Nile</strong> perch is normally collected from various l<strong>and</strong><strong>in</strong>g sites on shore <strong>and</strong> isl<strong>and</strong>s of<br />

<strong>Lake</strong> Victoria. Whole <strong>Nile</strong> perch is not washed nor guted at fish<strong>in</strong>g grounds/upstream. It is<br />

collected <strong>and</strong> iced <strong>in</strong> the transportation vessels, <strong>in</strong>sulated boats <strong>and</strong> trucks at isl<strong>and</strong>s or<br />

l<strong>and</strong><strong>in</strong>g sites. Usually collection vessels collect fish at m<strong>in</strong>imum of 3 days to maximum of 3<br />

weeks (21days), dur<strong>in</strong>g this period the fish is stored <strong>in</strong> ice until transported to the<br />

establishments. The fish is off loaded <strong>and</strong> washed at the receiv<strong>in</strong>g section of the<br />

establishments. There are at least two wash<strong>in</strong>g stages. At the first stage the fish is scrubbed<br />

us<strong>in</strong>g brush (coloured) <strong>and</strong> washed with runnig/shower water. Then it is r<strong>in</strong>sed <strong>in</strong> next<br />

(second) stage by another h<strong>and</strong>ler/person <strong>and</strong> f<strong>in</strong>ally size graded <strong>and</strong> placed <strong>in</strong> a conta<strong>in</strong>er<br />

(figure 14) with melt<strong>in</strong>g ice <strong>and</strong> stored chilled until they are subjected to alternative<br />

processes. The tables 18 <strong>and</strong> 19 below show the microbiological results for TVC <strong>and</strong><br />

Enterobacteriaceae before (BW) <strong>and</strong> after wash<strong>in</strong>g (AW) of whole <strong>Nile</strong> perch <strong>and</strong> onl<strong>in</strong>e<br />

fillets prior to the super chill<strong>in</strong>g stage at three fish process<strong>in</strong>g establishments (E1, E2, &<br />

E3).

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