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Microbiology and Spoilage Trail in Nile Perch (Lates niloticus), Lake ...

Microbiology and Spoilage Trail in Nile Perch (Lates niloticus), Lake ...

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4.3.2.4 Establishment E4 – chilled fillets (5 days iced whole <strong>Nile</strong> perch)<br />

Chilled <strong>Nile</strong> perch fillets were processed from a lot of whole <strong>Nile</strong> perch stored <strong>in</strong> ice for 5<br />

days. Changes for spoilage bacteria on chilled fillets were constant up to 13 storage days<br />

(figure 24 <strong>and</strong> 25). The trend was characterised by an <strong>in</strong>crease for both SSO <strong>and</strong> TVC <strong>in</strong><br />

parralel <strong>and</strong> reached a stationary phase after 13-15 storage days. The changes <strong>in</strong> TVB-N<br />

showed moderate <strong>in</strong>crease from the beg<strong>in</strong>n<strong>in</strong>g up to 13 days <strong>and</strong> then very rapid <strong>in</strong>crease<br />

between days 13-15. The SSO <strong>and</strong> TVC <strong>in</strong>creased from 3-7log10cfu/g <strong>in</strong> the first 10 days of<br />

storage <strong>and</strong> to 9 log10 cfu/g after 15 days while the Enterobacteriaceae counts gave only a<br />

slight <strong>in</strong>crease from 2.5-3.7 log10 cfu/g. Aga<strong>in</strong> these results <strong>in</strong>dicate that the SSO counts are<br />

closely equals to TVC counts between 13-15 storage days. The chemical (TVB-N) changes<br />

dur<strong>in</strong>g 15 days storage of chilled <strong>Nile</strong> perch fillets show an <strong>in</strong>crease from about 5-<br />

13mgN/100g up to 15 storage days (figure 25 <strong>and</strong> 26). The changes <strong>in</strong> pH shifted from 6.5-<br />

6.9 units dur<strong>in</strong>g 15 storage days.<br />

Figure 24: Changes of specific spoilage<br />

organism (SSO), total viable counts<br />

(TVC) <strong>and</strong> Enterobacteriaceae (Ent.) on<br />

chilled fillets.<br />

55<br />

Figure 25: Changes of TVB-N on chilled<br />

fillets.<br />

Figure 26: Changes of specific spoilage<br />

organism (SSO), total viable counts<br />

(TVC), Enterobacteriaceae (Ent.) <strong>and</strong><br />

TVB-N on chilled fillets.

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