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Urologia, Azienda Ospedaliera San Paolo, Milano - Polo Universitario, Milan, Italy Aim: The purpose of this study was to evaluate the role of prognostic factors in disease progression and cancer-specific mortality in patients undergoing radical cystectomy (RC) and bilateral pelvic lymphadenectomy (PLAD) for urothelial cancer. Patients and Methods: We retrospectively reviewed 116 RC + PLAD cases performed for urothelial bladder cancer at the San Paolo Hospital, University of Milan from February 2004 to April 2010. Of the 116 procedures, 80 were evaluable. All surgical procedures were performed by the same operator. Of the 80 patients, 60 were males (75%) and 20 females (20%). The mean age of patients was 70.3 (range 52-88) years and the mean follow-up was 31 (range 2-102) months. The criteria used to allocate patients to CR + PLAD were: muscleinvasive cancer, non muscle-invasive high-grade tumors (HG), recurrence or no response to immunoprophylaxis/therapy with Calmette-Guerin bacillus (BCG). The performed urinary diversions were 48 Wallace-type ureteroileocutaneostomy, 23 Studer-type orthotopic ileal neobladder, 2 vescica ileale Padovana, 8 ureterocutaneostomy and 1 nephrostomy. The prognostic factors evaluated were: age (cut-off
Abstracts of the 21st Annual Meeting of the Italian Society of Uro-Oncology (SIUrO), 22-24 June, 2011, Naples, Italy (Ypsilon Biotechnology, Napoli, Italy). Cells starved in red phenol-free DMEM and 1% charcoal treated FBS for five days were treated with 20 mM E 2 or 10 –6 or 10 –8 SOM230 or 20 mM E 2+ SOM230 (10 –8 or +10 –6 ) for 48 h. Cells were differently harvested for semi-quantitative RT-PCR, Western blot or flow cytometry (FACS) analyses. Results: E 2 induced an up-regulation of SSR 1, 2 and 5 mRNA and proteins. E 2 and SOM 10 –6 alone induced apoptosis and slightly reduced proliferation. The combined treatment of 20 mM E 2 + SOM230 (10 –8 or 10 –6 ) induced a stronger rate of apoptosis and a greater decrease of proliferation compared to either alone. The synergistic action of SOM230 and E 2 induced a reduction in S-phase proliferation with an arrest in G 0/G 1 phase. Caspase-dependent apoptosis was induced by SOM230. while a reduction of BCL-2 levels was induced after the addition of E 2, which amplified SOM230 effects at lower doses. Conclusion: E 2 increases the inhibitory effects of SOM230 in a prostate cell model expressing ER alpha and beta, acting directly on cell growth and cell death control and up-regulating SSRs. 1 Tang X, Takekoshi S, Itoh J, Umemura S, Shoji S, Terachi T and Osamura RY: Somatostatin analogue inhibits the mobility of prostate carcinoma cells: A new therapeutic method for advanced prostate carcinoma. Int J Oncol 37(5): 1077-1083, 2010. 2 He Y, Yuan XM, Lei P, Wu S, Xing W, Lan XL, Zhu HF, Huang T, Wang GB, An R, Zhang YX and Shen GX: The antiproliferative effects of somatostatin receptor subtype 2 in breast cancer cells. Acta Pharmacol Sin 30(7): 1053-1059, 2009. 3 Kimura N, Takamatsu N, Yaoita Y, Osamura RY and Kimura N: Identification of transcriptional regulatory elements in the human somatostatin receptor sst2 promoter and regions including estrogen response element half-site for estrogen activation. J Mol Endocrinol 40(2): 75-91, 2008. 68 PROKINETICIN 1 STIMULATES THE MIGRATION AND PROLIFERATION OF PROSTATE EPITHELIAL CELLS IN VITRO Giuseppe Bellastella1 , Valentina Rossi1 , Paolo Chieffi2 , Antonio Alberto Sinisi1 , Domenico Prezioso3 , Fabrizio Iacono3 , Ester Illiano3 , Carmine Cicalese3 and Raffaele Galasso3 1Endocrinologia, Dipartimento di Scienze Cardiotoraciche and 2Dipartimento di Medicina Sperimentale, Seconda Università di Napoli, Napoli, Italy; 3Clinica Urologia, Universitá Federico II Napoli, Napoli, Italy Background and Aim: Increased PROK1 expression has been found in prostate hyperplasia and cancer, suggesting a role in prostate proliferative diseases. The aim of this study was to elucidate the role of PROK1 on prostate cell (PC) function and growth. We evaluated the effects of PROK1 on epithelial PC migration and proliferation using the androgen-dependent epithelial PC line EPN, and a stabilized PC line derived from prostate cancer (CPEC). Materials and Methods: Semiconfluent starved cultures were treated with recombinant PROK1 (5 nM), alone or associated with antiPROK1 monoclonal antibody or solvent. Cells were harvested 48 h after the treatment and stained with propidium iodide for flow cytometry of the cell cycle distribution by FACSCalibur or recovered for protein extraction for Western blot analysis, or for mRNA extraction for semi-quantitative RT-PCR. Cells grown on slides were also treated and harvested after 46 h for TUNEL assay. A wound assay was performed for the evaluation of cell motility after overnight incubation. For ERK phosphorylation assay, cell cultures were recovered after 5, 10. 20 and 60 min following treatment. Results: An increase of the cell number in S phase, with a decrease of cell counts in pre-G 1 and G 0/G 1 and a significant reduction of the percentage of fragmented nuclei was found after PROK1 treatment (p
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Nucciotti R, 35 Oderda M, 37, 38, 1
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