Page 1 .76 .. 1993 ANNUAL MEETING ABSTRACTS Session A ...
Page 1 .76 .. 1993 ANNUAL MEETING ABSTRACTS Session A ...
Page 1 .76 .. 1993 ANNUAL MEETING ABSTRACTS Session A ...
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542<br />
Ra 0..... Oil-Com.,wllon Ind Cha~nislicL FnuIk T.ru-rer. Ri«Jand<br />
Foods. Inc., p.o. 8oJ. 927, Srungan. ~ 12160. and Raben J. Nicolosi.<br />
Univ.mty of M_~t5.I"ow(:Jl. MassxhuseIU01&54.<br />
Int~ in rice Inn oil,.."...enewed when rhe hypolipidemic propcniesofricc<br />
bnn. observed In ,,"imal and human trials, .. ...., ((lUnd to be roncrouated: in rhe oil<br />
componmts. StLKIies on bknded fa" &howePpin8 with .-4(pyridyl l-oxidc)-N-Iut·bulylnitronc (POBN). Lipid rldlcal<br />
form.tion Will' enh.""ed when the ,row!h medium WI. ""ppl.menled ... ilh a.<br />
lillie as 5 mM OOcO$llheueooic Kid (DHA. 22:6n_3). Computef simul.tion indi_<br />
caled Ihat It leOSI two c.rbon-eenIC"'" POBN spin adducts we'" formed. The<br />
spectral charKtcrillica ....en: consi.llcnt with lhe pn:Stoce of. pentenyl adduct<br />
(aN~15.15 0 and .H_2.61 OJ and an ethyl addotC\ (IN .. IS.13 G and all-2.78 OJ.<br />
These .Ikyl tMliuls probably are formed by jJ-..,ill$ion of.lkoAyl radic.l ..<br />
Allhough radical adduct rormation was not sp«ir", ror DHA. the amo\IIIt or Spill<br />
IMldUCtgenerllM wlS 1""ler when the «II. were .upp ....memed durinlll"Dwlh<br />
wilh n·) ... c:ontpllml with n·6 poIyuniollura,ed rltly .dds. Addilion of .oscorbale<br />
to the _y medium ,,,,ally ioc",ased the radical signal intensity. Radical ,mcro_<br />
tion .... s dependent on the presence of cells. bullhe POBN spin adduct was<br />
lktected primarily In lhe c11nocellullf fluid. COtTObonilion ofiron·induced lipid<br />
peroxidalion w'5 achlcved u,inl Ihe thiobarbiluric add n:"IC1ive substa.ncu .~say.<br />
These findings inditlle thRt lipid rodital formalion i. ~trongly