Page 1 .76 .. 1993 ANNUAL MEETING ABSTRACTS Session A ...

DO'"
Llpld-Sllrface OrpnlUlUon ProbftI by Upase-C.lIIlyud 180 Euhanr. J.M.
Mudc:rhw .. W.E.. MI)n1JCf)and IU .. Brockman. Univeniry ofMirmcsouo. The
HOI'TIKI [n$I;IUIe. 801 16th "venue HE.. Austin. MiJ\l1eSOUl55912.
The exdwlp of 180 bttween H 2 160 and p""labeUt:d domsadicnoic Kid
(180. J 80-010) caca/yad by euboKylesier lipase and roti~ 1iP"R wq
inw:~ ,,"m, monolayer and billyer immKe5. In rum. of ISo.I80-0A aJone.
the oxy..... uchanje ruction protccdcd by • rarUxn, ...qumrial medIanism and
was limi~ by the IICY.laIion ~ will! ke.JKm of 0.12-0.16 =2pmo!-1s- I. With
mixed monoIayer$ of 180, lllo·DA and !.paImiloyl-2-okoy1-phosphatidyIdloliM,
the Ulft'll of lipue-aulyud ~y ..... ~ inaeased abrupdy bet ........ O.sand
0.6 """'" f..roo;., of DA. Concomitaoll. with IhiI critiaI tramitiCIU ... I dIInge in
the ~ IIIfdwIism from coupled IOrandorII.. ~ Re5ultswllh unilamel·
.. bilIIycQ ~ ..... ;1... nkcn 1Of"Iher. the com""';'~ swildlina of
ItCIivily and runion mechanism IUgati R'gulQ:III by the bunl orpni1.:Mion of
lipid hud-JIOIlPf in the inI«f_ Sud\. mnsiIion is bc:R IIDd=uIod in the IXInIUI
01 pcra>UbDn Ihro;wy and _Id play .. imporlld mil: in lipid-mtdialcd sip! uans-
......
0005
Soh.blllliel of SImple and Mind TriKyl&l}Unlls in Sapertttlkal Fluid COz.
W.B. Niluoo and J.K. Hudson. NOAA. NMFS, NW FISberie$ Sci. Ctr~UrilUMion
Rc:xaR:h I)jviJion. 2m Monllake IIQgLevatd &.I, SealtIc. WI. 98112.
The puI dec:wIo: hu .... ;messed aro"'illl im~ inthe appl~ion of 0..pt1l:ril;.
tal nuid (SF) Ulrxlion 10the proea!Iing of edible oils_ A complele undenlanding
of these ~ ..qui .... fundamental dua \0 ctw.:taize the behavior of ")'SIem!l
tmSisting of ';pick and IUptl'Critlcal n..ids. For eumpk. a rwmbo:r of stuWQ h.lve
reported iOIubilllla of.implo triacylJlycerols (TAGs) IUCb as Iripalmit)'lglyecroI
(PPP) and IriokyIJlym'Ol (000). SIICh simp\t: TAGs are no! normally Ihe prcdomi_
IWII ~ in nMUnII oil$. which mainlya>nlllia mixed TAGs IUCb III l-pllmit)'ldioIeylJlym'Ol
(1'00). A model miann. ~ of PPP. 000. 1'00 and PPO
_ .yn~ cncyraalicaUy. By·produls) wen: =nChemicaIprdermco of ~
_ Iynthetiad Ihe tnIIUiomericall JIUft ed!y\ ,,·huyl p-niuopbenypho5pbclnMe
..hidI_ found 10 be suiubk in Ihe dr:tcnninltlon of li~ inhibition ltindia.
Kinetic studies cIC. fINDIrfica tiptie ohowcd UI CfIIntiolcJecrlve inhibiDon K2i'Kd
• ] 1.4 mM-I.-1 for Ihe (.)~ and K2t'KcI- 1.1 mM-I.-I for the(+) CM!'Itlomer.
1bua. • learic acid (12·HSA) forms. thermon:vCl'Jibie 8"1 ....ith ph)'$ical
lUMIciotion nttWOl'l.: in orpnic soIvenII. In the prescroce of a small amowu ...- alkali
melll ion. we h.lve round a fomwion of IIO~I 8"11 II It>w conc:entntion of 12_HS.
The "",wort 1ItNCIW'e .... cum'nr:d by pIwc diagams, ein:ular dichroi .... (CD).
'nf!Wed r.pecI/I (IR) and electron microloope (EM). Alkali mt1al soaps were wed 15
a soutre cIalkali mcIII iom. The uperlmcnu wen: curled Out in the DCJRCIetIlrIIim
ronae of 1-3""'- 12-HSA for !he system 12-HSAhodium 12·ItSAhoyban oil. The
optimal molar lIlloofI2-HSA/N.+ with I'1:5]lcct to ,eI fOfmllion wu 100,1. Whi\t:
the muimum .... ~lensth of CD opa:UUm derived from lhechira!ity or 12_HSA was
kepi COI\SIInI, lhe mnarbble molarellipticilY [8[ decrease wasohscrvcd with an
inc~_ in the conccntnuion of N.... IR opu!lI indicated lhallhe inten.ity of
absorplion bands due 10 the h)'drogm bonded ..)'dto~yl &fOOl" was kept oomtant
with the addiliorl of NI+, Funhermon:. the fill(ly dilpersed fillets W