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SUMO PPI project • PI: Yuan Chen, PhD (City of Hope, Duarte, CA) • MLPCN grant R21 NS066498-01 Song et al, PNAS, 2004 Song et al, JBC, 2005
Assay design for SUMO PPI project Biological reagents: • SUMO1-GST • SUMO2-GST • SUMO3-GST • FITC-S1 peptide (F-S1) • S1-FITC peptide (S1-F) • FITC-S2 peptide (F-S2) Detection reagents: • Lumi4® Tb anti-GST (CisBio; cat# 61GSTTLA) Tested a matrix of two SIM peptides and three SUMO paralogues in three assay formats Established two potential pairs for further assay development: SUMO1/S1 and SUMO2/S1
Page 1 and 2: HTRF Symposium Avignon, France Apri
Page 3 and 4: • SBMRI and CPCCG • NIH Roadmap
Page 5 and 6: Development of Sanford-Burnham Medi
Page 7 and 8: Sanford-Burnham’s Discovery Resea
Page 9 and 10: Conrad Prebys Center for Chemical G
Page 11 and 12: Sanford-Burnham uHTS Facility (Orla
Page 13 and 14: Specifics of MLPCN projects • MLP
Page 15 and 16: MLPCN screening at CPCCG Detection
Page 17 and 18: MLPCN UBL projects performed at CPC
Page 19: SUMO PPI project • PI: Yuan Chen,
Page 23 and 24: SUMO-PPI MLPCN screening project
Page 25 and 26: SUMOylation project: HTRF Assay GST
Page 27 and 28: SUMOylation project: Probe compound
Page 29 and 30: • Assay Reagents: Assay captures
Page 31 and 32: R a tio (5 2 0 /4 9 0 )x 1 0 0 0 0
Page 33 and 34: Z' factor CUL1 neddylation project
Page 35 and 36: MLN4924 has a complex mechanism of
Page 37 and 38: Concluding remarks • HTRF approac

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