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• PI: Matt Petroski (SBMRI) CUL1 Neddylation Project • The assay targeting NAE and Ubc12 designed and developed in collaboration with PI
• Assay Reagents: Assay captures CUL1 neddylation • NAE (His6-tag) and Ubc12 (His6-tag) • SCF (Skp1-CUL1-F-box) – CUL1 is FLAG-tagged • Nedd8 Fluorescein-Nedd8 Biotin-Nedd8 • Anti-FLAG antibodies Eu-anti-FLAG (Life Tech) Lumi4®-Tb anti-FLAG (CisBio) Streptavidin DyLight650 (Pierce; cat #84547) Streptavidin DyLight488 (Pierce; cat #21832) FITC Nedd8 O NH 2 Lys CUL1 (SCF) FLAG OH ATP AMP FITC Nedd8 O NH NAE Ubc12 CUL1 (SCF) FLAG
Page 1 and 2: HTRF Symposium Avignon, France Apri
Page 3 and 4: • SBMRI and CPCCG • NIH Roadmap
Page 5 and 6: Development of Sanford-Burnham Medi
Page 7 and 8: Sanford-Burnham’s Discovery Resea
Page 9 and 10: Conrad Prebys Center for Chemical G
Page 11 and 12: Sanford-Burnham uHTS Facility (Orla
Page 13 and 14: Specifics of MLPCN projects • MLP
Page 15 and 16: MLPCN screening at CPCCG Detection
Page 17 and 18: MLPCN UBL projects performed at CPC
Page 19 and 20: SUMO PPI project • PI: Yuan Chen,
Page 21 and 22: Assay design for SUMO PPI project B
Page 23 and 24: SUMO-PPI MLPCN screening project
Page 25 and 26: SUMOylation project: HTRF Assay GST
Page 27: SUMOylation project: Probe compound
Page 31 and 32: R a tio (5 2 0 /4 9 0 )x 1 0 0 0 0
Page 33 and 34: Z' factor CUL1 neddylation project
Page 35 and 36: MLN4924 has a complex mechanism of
Page 37 and 38: Concluding remarks • HTRF approac

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