Quattro 2 User's Guide, issue 2. 6666533 - Waters

Quattro II
User's Guide
Electrospray
Introduction 55
Post-column Splitting 57
Megaflow 58
Changing Between Flow Modes 58
The Triaxial Flow Probe 59
The CE Probe 59
Operation 60
Obtaining an Ion Beam 60
Source Voltages 62
CE Buffers and Make-up Solvents 62
Tuning and Optimisation 63
Probe Position 63
Setting Up the Probe 63
Coaxial Probe 63
Triaxial Probe 64
CE Probe 64
Sample Cone Position 66
Operating Parameters 66
Drying Gas Flow 66
ESI Nebulising Gas Flow 66
Probe (Capillary) Voltage 66
Counter Electrode (HV Lens) Voltage 67
Sample Cone Voltage 67
Skimmer and RF Lens 67
Skimmer Lens Voltage 67
Low Mass Resolution and High Mass Resolution 67
Ion Energy 67
Source Temperature 67
Megaflow Hints 68
Tuning for CE Operation 70
Removing the Probe 70
Sample Analysis and Calibration 71
General Information 71
Typical Positive Ion Samples 71
Typical Negative Ion Samples 71
Horse Heart Myoglobin 72
Polyethylene Glycol (PEG) 300/600/1000/1500 Mixture 74
Sugar Mixture 75
Sodium Iodide and Caesium Iodide Mixture 76
Positive Ion 76
Negative Ion 77
Chromatographic Interfacing 78
LC-MS Sensitivity Enhancement 79
Running CE Samples 79
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