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Quattro 2 User's Guide, issue 2. 6666533 - Waters

Quattro 2 User's Guide, issue 2. 6666533 - Waters

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<strong>Quattro</strong> II<br />

<strong>User's</strong> <strong>Guide</strong><br />

Pumping Down<br />

For API operation check that the source is correctly configured for the chosen<br />

mode of operation. (See “Changing Between Electrospray and APcI” later in<br />

this chapter.)<br />

For non-API operation, insert the chosen ion source into the mass spectrometer.<br />

Connect the GC column (if required).<br />

Connect the LC transfer line (if required).<br />

Connect the source connector plugs. (Failure to do so will cause MassLynx not<br />

to recognise the ion source in the mass spectrometer.)<br />

Connect all gas supply and pumping lines to the source.<br />

The API gas lines are connected simply by pushing the tubes into the coloured<br />

collars on the bulkhead connectors.<br />

APcI<br />

Probe<br />

Nebuliser<br />

Gas (Green)<br />

HPLC<br />

Column<br />

Electrospray<br />

Exhaust<br />

(Black)<br />

Drying<br />

Gas<br />

(White)<br />

Sheath<br />

Gas<br />

(Yellow)<br />

Injector<br />

Probe<br />

HPLC<br />

Column<br />

HPLC<br />

Pump<br />

Drying<br />

Gas<br />

(White)<br />

Nebuliser<br />

Gas (Red)<br />

Injector<br />

Nitrogen<br />

Solvent<br />

Non-return<br />

Valves<br />

HPLC<br />

Pump<br />

Exhaust<br />

(Black)<br />

Nitrogen<br />

Solvent<br />

Routine Procedures<br />

Page 48

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