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53. Volume 13- Number 1 - IP Australia

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PLANT VARIETIES JOURNAL 2000 VOL <strong>13</strong> NO. 1<br />

Table 18 Continued<br />

LEAF COLOUR (RHS, 1995) centre secondary colour<br />

green yellow-green yellow-green<br />

<strong>13</strong>7C 147C 146D<br />

____________________________________________________<br />

FLOWER BUD COLOUR (RHS, 1995)<br />

greyed-purple yellow yellow-green<br />

185B <strong>13</strong>D 149B<br />

____________________________________________________<br />

FLOWER PETAL COLOUR (RHS, 1995)<br />

red white white<br />

55B 155C 155C<br />

____________________________________________________<br />

LEAF TWISTING<br />

absent absent present<br />

____________________________________________________<br />

STEM COLOURATION<br />

green green yellow-green<br />

_______________________________________________<br />

Gossypium hirsutum<br />

Cotton<br />

‘Sicala V-2RR’<br />

Application No: 1999/036 Accepted: 16 March 1999.<br />

Applicant: CSIRO Plant Industry, Cotton Research<br />

Unit, Narrabri, NSW.<br />

Characteristics (Table 19, Figure 42) Plant: shape conical,<br />

height medium (mean 95.9cm), medium maturity (178 days<br />

to mature), medium foliage density. Leaf: palmate, very<br />

slight pubescence of midrib, gossypol and nectary glands<br />

present. Flower: colour of petals cream. Boll: size large,<br />

shape elliptical, pitting of surface fine, length of peduncle<br />

medium (mean 25mm), prominence of tip medium, opening<br />

medium, bract size large (44x29mm). Seeds: density of fuzz<br />

medium. Lint: proportion high (0.40), length medium<br />

(28.8mm), strength high (32g/tex), micronaire value<br />

medium (3.4). Disease reaction: resistant to bacterial blight<br />

(Xanthomonas campestris pv malvacearum), good<br />

tolerance to verticillium wilt (Verticillium dahliae).<br />

Herbicide reaction: tolerant to glyphosate.<br />

Origin and Breeding Controlled pollination: seed parent<br />

94608 x pollen parent ‘Sicala V-2’ A at <strong>Australia</strong>n Cotton<br />

Research Institute (ACRI), Narrabri, NSW. The seed parent<br />

is distinguished by its segregating glyphosate tolerance. The<br />

pollen parent is distinguished by its susceptibility to<br />

glyphosate. This cross was the third backcross of ‘Sicala V-<br />

2’ A onto a line transformed with a Monsanto glyphosate<br />

tolerance gene. The first cross was carried out at St. Louis,<br />

USA and the F1 sent to quarantine at CSIRO Plant Industry<br />

in Canberra, <strong>Australia</strong> where the first backcross was carried<br />

out using ‘Sicala V-2’ A . Two subsequent backcrosses using<br />

‘Sicala V-2’ A as the recurrent parent were carried out at<br />

ACRI. At all stages progeny were screened for the<br />

glyphosate tolerance gene and a marker gene. Following the<br />

final backcross selfing was done and single plant selection<br />

followed by progeny row and multiple environment trials<br />

were carried out. Selection criteria: glyphosate tolerance,<br />

resistance to bacterial blight and Verticillium wilt, leaf<br />

hairiness, fibre quality and yield. Propagation: by seed.<br />

Breeder: Mr P E Reid, CSIRO Plant Industry, Cotton<br />

Research Unit, Narrabri, NSW.<br />

Choice of Comparators ‘Sicala V-2’ A was chosen because<br />

it is the recurrent parent and the most similar variety of<br />

common knowledge. The parent 94608 was not considered<br />

as a comparator because it is easily distinguished from<br />

‘Sicala V-2RR’ because of its segregating glyphosate<br />

tolerance.<br />

Comparative Trials Comparator: ‘Sicala V-2’ A . Trial<br />

location: ACRI, Narrabri, NSW, 1998 - 99 summer.<br />

Morphology and fibre quality trial conditions: field grown<br />

irrigated trial with conventional management. Trial design:<br />

15 entry trial in a row and column design with three<br />

replicates and three row x 14m plots. Measurements:<br />

morphological measurements on 10 plants from each plot.<br />

Lint % and fibre quality measurements taken on a 50 boll<br />

sample from the centre row of each plot. Fibre quality was<br />

measured on a Zellweger Uster HVI 900 instrument.<br />

Glyphosate tolerance trial conditions: glasshouse trial using<br />

255mm pots. Trial design: four pots containing four plants<br />

each for each treatment in a completely randomised design.<br />

Standard commercial dose of Glyphosate was sprayed onto<br />

plants at the 4 leaf stage.<br />

Prior Application and sales Nil.<br />

Description: Peter Reid, CSIRO Plant Industry, Cotton Research Unit,<br />

Narrabri, NSW.<br />

Table 19 Gossypium varieties<br />

‘Sicala V-2RR’ *‘Sicala V-2’ A<br />

____________________________________________________<br />

PLANT WILTING 7 DAYS AFTER GLYPHOSATE<br />

APPLICATION<br />

no wilting severe wilting<br />

____________________________________________________<br />

PLANT DAMAGE 14 DAYS AFTER GLYPHOSATE<br />

APPLICATION<br />

no damage dead<br />

_______________________________________________<br />

‘Sicot 189RR’<br />

Application No: 1999/037 Accepted: 16 March 1999.<br />

Applicant: CSIRO Plant Industry, Cotton Research<br />

Unit, Narrabri, NSW.<br />

Characteristics (Table 20, Figure 43) Plant: shape conical,<br />

height medium (mean 91.9cm), late maturity (180 days to<br />

mature), medium foliage density. Leaf: palmate, very slight<br />

pubescence of midrib, gossypol and nectary glands present.<br />

Flower: colour of petals cream. Boll: size medium, shape<br />

elliptical, pitting of surface fine, length of peduncle medium<br />

(mean 24mm), prominence of tip medium, opening<br />

medium, bract size medium (41x24 mm). Seeds: density of<br />

fuzz medium. Lint: proportion high (0.38), length medium<br />

(29.8mm), strength high (33g/tex), micronaire value<br />

medium (3.4). Disease reaction: resistant to bacterial blight<br />

(Xanthomonas campestris pv malvacearum), good<br />

tolerance to verticillium wilt (Verticillium dahliae).<br />

Herbicide reaction: tolerant to glyphosate.<br />

38

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