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53. Volume 13- Number 1 - IP Australia

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DESCR<strong>IP</strong>TIONS<br />

PLANT VARIETIES JOURNAL 2000 VOL <strong>13</strong> NO. 1<br />

Table 52b Trifolium varieties<br />

Rust resistance trial<br />

‘Lightning’ *‘Laser’ *‘Leeton’ *‘Maral’ *‘Felix’ *‘Stemher’ *‘Lupers’ *‘Archibald’<br />

______________________________________________________________________________________________________________<br />

RUST RESISTANCE (rating)<br />

mean 8.0 6.0 3.5 6.75 7.25 6.25 7.75 6.25<br />

std deviation 0.8 0.5 1.0 0.5 0.5 0.9 0.9 1.6<br />

LSD/sig 1.3 P≤0.01 P≤0.01 ns ns P≤0.01 ns P≤0.01<br />

______________________________________________________________________________________________________________<br />

Trifolium subterraneum subsp brachycalycinum<br />

Subterranean Clover<br />

‘Antas’<br />

Application No: 1999/147 Accepted: 16 Mar 2000.<br />

Applicant: Istituto Sperimentale per le Colture<br />

Foraggere, Lodi, Italy.<br />

Agent: SEEDCO, Hilton, SA.<br />

Characteristics (Table 53, Figure 54) Seedling: moderately<br />

upright, medium to large, vigorous. Plant: dense, spreading,<br />

prostrate to semi-prostrate, late maturing. Stem: glabrous<br />

with rare hairs, green, some red (anthocyanin) pigmentation<br />

where exposed to sun in spring. Petioles: sparsely to<br />

moderately pubescent, green or with red pigmentation as<br />

for stems. Leaf: large, moderately pubescent lower, sparsely<br />

pubescent upper surface. Leaf mark: pale green crescent<br />

and arms (C 1 A 1-2 type, Nicholls et al, 1996), but crescent<br />

may be faint or absent in some stages, no fleck or flush.<br />

Stipules: green with lower red pigmented veins. Peduncle:<br />

medium to long, moderately pubescent, green or red<br />

pigmented where exposed to sun. Inflorescence: spikelet of<br />

4 to 5 florets, white to pink. Seed: large, black or dark<br />

purplish-brown. Burr: burial poor, calyx teeth moderately<br />

pubescent.<br />

Origin and Breeding Phenotypic selection: selection from<br />

a variable population designated as EP 19, segregating for<br />

flowering time, growth habit and leaf markers. Segregates<br />

were isolated, multiplied and trialed over 10 years and<br />

tested for persistance and seed yield in Sardinia. A number<br />

of elite lines were selected and then tested in various trials<br />

in Western <strong>Australia</strong>. One line, EP 19 brachy E was found<br />

to produce more herbage and burr, to have a higher<br />

percentage of hard seed at harvest and to regenerate more<br />

strongly than ‘Clare’. EP 19 brachy E was then used to<br />

develop the uniform single line, ‘Antas’ through single plant<br />

selection. Selection criteria: winter vigour, dry matter yield,<br />

seed and burr yield, regeneration, persistence. Propagation:<br />

by seed. Breeder: Dr Efisio Piano and staff, Istituto<br />

Sperimentale per le Colture Foraggere, Lodi, Italy.<br />

Choice of Comparators ‘Rosedale’, ‘Clare’ and ‘Nuba’ A<br />

were initially considered for the comparative trial as these<br />

are the only commonly available varieties of Trifolium<br />

subterraneum ssp brachycalicinum. ‘Rosedale’ was<br />

excluded as a comparator, as it is clearly distinguishable<br />

from ‘Antas’ in having cream to white seeds (‘Antas’ has<br />

dark purplish-brown to black seeds) and flowers over three<br />

weeks earlier than ‘Antas’. ‘Clare’ and ‘Nuba’ A have<br />

similar seed colour to and in most circumstances flower<br />

within 10 days of ‘Antas’, and were therefore chosen as<br />

comparators. The original source material (EP 19) was not<br />

considered because it is a heterogenous population and does<br />

not have well-defined C 1 A 1-2 type leaf markings.<br />

Comparative Trial Comparators: ‘Clare’, ‘Nuba’ A .<br />

Location: Currency Creek, about 75km SSE of Adelaide,<br />

SA, between Jun and Nov 1999. Conditions: trial conducted<br />

in the field. The soil was a moderately fertile, free draining<br />

sandy loam of approximately pH 6. A single spring<br />

irrigation of approximately 40mm rainfall equivalent was<br />

applied in mid Oct to allow plots to mature with minimum<br />

water stress. No chemical or fertiliser treatments were used<br />

and plots were hand weeded as required. Trial design: a<br />

randomised complete block with 4 replicates, each of 10<br />

plants. Plants were seeded and raised in Jiffy 7 pellets in a<br />

shadehouse, and then transplanted into the field at<br />

approximately 4 weeks of age in late Jun, 1999. Each<br />

replicate was comprised of 10 plants in 4 rows, with 20 cm<br />

between plants and 50 cm between rows. Measurements:<br />

from all plants.<br />

Prior Applications and Sales Nil.<br />

Description: Andrew W.H. Lake, Pristine Forage Technologies, Daw<br />

Park, SA.<br />

Table 53 Trifolium varieties<br />

‘Antas’ *‘Clare’ *‘Nuba’ A<br />

____________________________________________________<br />

DAYS TO FIRST FLOWER – days from germination in early<br />

June to first open floret<br />

mean 126.23 122.75 129.23<br />

std deviation 0.287 0.661 0.320<br />

LSD/sig 0.87 P≤0.01 P≤0.01<br />

____________________________________________________<br />

WINTER PETIOLE COLOR/PIGMENTATION<br />

green red-purple red-purple<br />

____________________________________________________<br />

PROXIMAL ANTHOCYANIN FLUSH ON LEAFLET<br />

absent present absent<br />

____________________________________________________<br />

LEAF MARK TYPE (Nicholls et al, 1996)*<br />

C 1 A 1-2 C 3 A 2-3 C 1 A 2<br />

____________________________________________________<br />

LEAF MARK PROMINENCE<br />

faint prominent faint<br />

____________________________________________________<br />

FLORET COLOUR<br />

white or pink white white<br />

____________________________________________________<br />

* Nicholls P.G.H., Collins W.J. and Barbetti M.J. (1996).<br />

Registered cultivars of subterranean clover.<br />

Bulletin number 4327, Agriculture Western <strong>Australia</strong>.<br />

73

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