optical interference filters - SPOT Imaging Solutions
optical interference filters - SPOT Imaging Solutions
optical interference filters - SPOT Imaging Solutions
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Optimize Your System<br />
with the Right Filter Set<br />
application note Optimize Your System with the Right Filter Set<br />
multi-band sets include increased filter cost and the inability to<br />
image multiple colors simultaneously. Instead, commercially available<br />
imaging software can be used to merge separate images.<br />
Fluorescence in situ hybridization (FISH) applications attempt to<br />
image as many colors as possible in a single sample. For example,<br />
multiple fluorescent labeled DNA probes can identify genes colorimetrically<br />
on a single chromosome. Optimized signal-to-noise ratio<br />
and color discrimination require narrowband single- dye filter sets.<br />
The <strong>filters</strong> must conform to tighter spectral tolerances than standard<br />
bandpass filter sets to minimize excitation/emission overlap<br />
of spectrally close fluorophores. Minor passband edge shifts may<br />
significantly compromise fluorophore discrimination. In addition,<br />
these narrowband <strong>filters</strong> must be optimized for transmission to provide<br />
adequate signal.<br />
Choosing <strong>optical</strong> filter sets for fluorescence microscopy can be<br />
confusing. Proper bandwidths, degree and extent of blocking, and<br />
80<br />
the type of filter design for your application are important considerations.<br />
We can help with your decision-making. Please feel free to<br />
contact us for assistance.<br />
70<br />
100<br />
90<br />
Transmission (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
450 500 550 600 650 700 750<br />
Wavelength (nm)<br />
XF<br />
XF<br />
XF<br />
Sp<br />
Ex<br />
Sp<br />
Em<br />
60<br />
50<br />
40<br />
30<br />
20<br />
Figure 6 <br />
Filter sets used in mFISH assays exhibit narrow<br />
filter bandwidths for minimizing spectral<br />
bleedthrough of non-specfic fluorophores and<br />
accurate color reperesentation. Reperesented<br />
here is Omega filter set XF424 for Spectrum Red,<br />
Texas Red, and similar fluors.<br />
XF1424 580QM30<br />
XF3418 630QM36<br />
XF2029 595DRLP<br />
Spectrum Red<br />
Excitation<br />
Spectrum Red<br />
Emission<br />
10<br />
0<br />
450 500 550 600 650 700 750<br />
102<br />
For current product listings, specifications, and pricing:<br />
www.omega<strong>filters</strong>.com • sales@omega<strong>filters</strong>.com<br />
1.866.488.1064 (toll free within USA only) • +1.802.254.2690 (outside USA)
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