optical interference filters - SPOT Imaging Solutions

More documents

Recommendations

Info

Fluorescence Filter setS Reference Table Fluorescence Filter Sets Reference Table Filter Set SKU Product Category Page # Filter Set SKU Product Category Page # Filter Set SKU Product Category Page # XF401 QuantaMAX, M-FISH 72,79 XF13-2 Standard 73 XF305-1 Quantum Dots 93 XF402 QuantaMAX 72 XF135 Multi-band - Dual 78 XF305-2 Quantum Dots 93 XF403 QuantaMAX, M-FISH 72,79 XF135-1 Pinkel 92 XF306-1 Quantum Dots 93 XF404 QuantaMAX 72 XF138-2 Standard 75 XF306-2 Quantum Dots 93 XF405 QuantaMAX 72 XF140-2 Standard 75 XF307-1 Quantum Dots 93 XF406 QuantaMAX 72 XF141-2 Standard 75 XF307-2 Quantum Dots 93 XF407 QuantaMAX 72 XF14-2 Standard 73 XF308-1 Quantum Dots 93 XF408 QuantaMAX, M-FISH 72,79 XF142-2 Standard 75 XF308-2 Quantum Dots 93 XF409 QuantaMAX 72 XF148 Standard 75 XF309-1 Quantum Dots 93 XF410 QuantaMAX 72 XF149 Standard 73 XF309-2 Quantum Dots 93 XF411 QuantaMAX 72 XF151-2 FRET 88 XF320 Quantum Dots 94 XF412 QuantaMAX 72 XF152-2 FRET 88 XF32 Standard 76 XF413 QuantaMAX 72 XF154-1 Pinkel 92 XF35 Standard 76 XF414 QuantaMAX 72 XF155 Sedat 95 XF37 Standard 76 XF416 QuantaMAX 72 XF156 Sedat 95 XF38 Standard 76 XF421 QuantaMAX M-FISH 79 XF157 Sedat 95 XF40-2 Standard 74 XF422 QuantaMAX M-FISH 79 XF158 FRET 88 XF43 Standard 76 XF424 QuantaMAX M-FISH 79 XF159 FRET 88 XF45 Standard 76 XF425 QuantaMAX M-FISH 79 XF16 Ratio Imaging 96 XF46 Standard 76 XF452 QuantaMAX Dual Band 91 XF160 FRET 88 XF47 Standard 76 XF453 QuantaMAX Dual Band 77 XF162 FRET 88 XF48-2 Standard 75 XF454 QuantaMAX Dual Band 77 XF163 FRET 88 XF50 Multi-band - Dual 78 XF467 QuantaMAX Triple Band 77 XF164 FRET 88 XF50-1 Pinkel 91 XF452-1 Pinkel 91 XF165 FRET 88 XF52 Multi-band - Dual 78 XF453-1 Pinkel 91 XF166 FRET 88 XF52-1 Pinkel 91 XF454-1 Pinkel 91 XF167 FRET 88 XF53 Multi-band - Dual 78 XF467-1 Pinkel, M-FISH 79,91 XF173 Standard 74 XF53-1 Pinkel 91 XF02-2 Standard 73,94 XF175 Standard 74 XF56 Multi-band - Triple 78 XF04-2 Ratio Imaging 96 XF179 Standard 76 XF57 Multi-band - Quad Set 78 XF05-2 Quantum Dots 73,94 XF18-2 Standard 73 XF57-1 Pinkel 92 XF06 Standard 73,80 XF201 M-FISH 80 XF59-1 Pinkel 91 XF09 Standard 76 XF202 M-FISH 80 XF63 Multi-band - Triple 78 XF100-2 Standard 74 XF203 M-FISH 80 XF63-1 Pinkel 92 XF100-3 Standard 74 XF204 M-FISH 80 XF66 Multi-band - Triple 78 XF101-2 Standard 74 XF206 M-FISH 80 XF67 Multi-band - Triple 78 XF102-2 Standard 74 XF207 M-FISH 80 XF67-1 Pinkel 92 XF103-2 Standard 74 XF208 M-FISH 80 XF68 Multi-band - Triple 78 XF104-2 Standard 74 XF21 Standard 76 XF68-1 Pinkel 92 XF105-2 Standard 74 XF23 Standard 74 XF69 Multi-band - Triple 78 XF106-2 Standard 73 XF25 Standard 76 XF69-1 Pinkel 92 XF108-2 Standard 74 XF300 Quantum Dots 93 XF72 Ratio Imaging 96 XF110-2 Standard 75 XF301-1 Quantum Dots 93 XF76 Standard 76 XF111-2 Standard 74 XF301-2 Quantum Dots 93 XF88-2 FRET 88 XF114-2 Standard 73 XF302-1 Quantum Dots 93 XF89-2 FRET 88 XF115-2 Standard 73 XF302-2 Quantum Dots 93 XF92 Multi-band - Dual 78 XF116-2 Standard 74 XF303-1 Quantum Dots 93 XF93 Multi-band - Triple 78 XF119-2 Standard 73 XF303-2 Quantum Dots 93 XF93-1 Pinkel 92 XF130-2 Standard 73 XF304-1 Quantum Dots 93 XF131 Standard 73 XF304-2 Quantum Dots 93 68 For current product listings, specifications, and pricing: www.omegafilters.com • sales@omegafilters.com 1.866.488.1064 (toll free within USA only) • +1.802.254.2690 (outside USA)
QuantaMAX and Standard Filters for Fluorescence Our fluorescence filter product line is comprised of Stock QuantaMAX and Standard Vivid and Basic excitation, emission and dichroic interference filters, and filter sets. For the visualization of fluorescence and imaging from deep UV absorbing compounds such as the aromatic amino acids Tyrosine and Tryptophan, to near IR absorbing dyes such as Indocyanine Green (ICG) Omega Optical offers a variety of interference filters and filter sets We have an impressive history of collaborating with researchers to identify filters that are uniquely compatible with specific fluorophores, as well as filters that are effective for fluorophores in a particular experimental design and optical set-up. These products are produced utilizing our multiple coating technologies, ion- assist, magnetron sputtering and physical vapor deposition, to best match the filter specifications to the application. QuantaMAX - Stock Interference Filters QuantaMAX are individual excitation, emission and dichroic filters and filter sets designed around the most commonly used fluorophores used in fluorescence detection and imaging. QuantaMAX (QMAX) filters are engineered and manufactured to meet the increased demands required of today’s imaging systems. Fluorophore Optimized: Organic fluorophores, whether a small molecule such as a cyanine dye, or a larger mass protein, such as e-GFP, absorb and emit photons in a highly wavelength dependent manner. This characteristic of a fluorescent compound can be illustrated by its specific fluorescence spectral curve, which describes the relative probabilities of the absorption and emission of photons across the wavelength spectrum. Figure 1 shows the excitation and emission curve for Cy5. This fluorophore is widely used in fluorescence techniques and exhibits an excitation absorption maximum at 649nm and emission maximum at 670nm. The slight separation of the two is called the Stokes shift and provides a spectral “window” through which researchers can (through the use of the appropriate interference filters) separate the incoming excitation light from the emitted fluorescence. deep out of band blocking are considerable, as generating high image contrast at low excitation light levels is a desirable condition in many protocols, particularly live cell imaging. The ability to place the excitation and emission filter pair’s passbands very close to the absorption and emission maximums of a particular fluorophore is a critical feature for obtaining this contrast. A filter set’s critical edges (the facing edges of the excitation and emission filters) are designed with a slope of 1% or less to allow for the closest placement of the two filters without sacrificing excitation light attenuation. (See figure 2 and 3) QuantaMAX - Stock interference filter sets provide optimal pass band placement to achieve efficient specific photon collection while simultaneously rejecting stray light and minimizing spectral bleed-through from spectrally close fluorophores. Figure 2 Transmission curve of XF407 set for Cy5 (showing steep edge slope) Figure 1 Excitation and Emission curves of Cy5 Given the small Stokes Shift of 20 nm or less of many of the typical fluorophores used in fluorescence systems, the demands placed on the filters to provide high transmission in the passband and Substrate Specifications: Each filter is produced on a single substrate which has been polished to < 15 arc seconds or better. This allows for minimal beam deviation and in most imaging systems leads to registrations shifts of 1 pixel or less. Excitation and emission filter substrates utilize a range of optical substrates which are optimized for low light scatter and high transmission through the pass band region of the filter. The use of certain high quality absorption glasses in the For current product listings, specifications, and pricing: www.omegafilters.com • sales@omegafilters.com 1.866.488.1064 (toll free within USA only) • +1.802.254.2690 (outside USA) 69
Page 1 and 2:
optical interference filters For Li
Page 3 and 4:
table of contents Laser Line Filter
Page 5 and 6:
about us Collaboration In any inst
Page 7 and 8:
Capabilities Design • Thin Film D
Page 9 and 10:
intellectual property 3 RD Millenni
Page 11 and 12:
photonics teaching kit Involved in
Page 13 and 14:
Longpass & Shortpass Filters 515ALP
Page 15 and 16:
Lot to Lot Reproducibility With the
Page 17 and 18: ColorMAX series is intended for spe
Page 19 and 20: Organic Semiconductor Overview Orga
Page 21 and 22: Filter design All boundaries betwee
Page 23 and 24: With Optical Monitoring, the intens
Page 25 and 26: highest spectral performance. Resea
Page 27 and 28: exposure to light, particularly sho
Page 29 and 30: Types of Anti-Reflective Treatments
Page 31 and 32: Figure 4 Multi-layer broadband anti
Page 33 and 34: Filter Design Considerations and Yo
Page 35 and 36: Optical Interference Filters for Ap
Page 37 and 38: Shack-Hartmann Method “Shack-Hart
Page 39 and 40: Stock and Standard products For a q
Page 41 and 42: Stock and Standard products 510BP3
Page 43 and 44: Stock and Standard products 850WB25
Page 45 and 46: Astronomy filters Throughout our hi
Page 47 and 48: Astronomy filters Projects Omega Op
Page 49 and 50: Our line of standard interference f
Page 53 and 54: For instrument developers: High vol
Page 55 and 56: Improved signal-to-noise Excellent
Page 57 and 58: Centered on the laser resonance Cl
Page 61 and 62: Full Width Half Max (FWHM) or bandw
Page 63 and 64: Steep slopes Specified by the crit
Page 65 and 66: UV capabilities We currently offer
Page 67: Dichroic Beamsplitters by Cut-On Pr
Page 71 and 72: Summary Given the vast number of fl
Page 77 and 78: Steep edges Exceptional transmissi
Page 79 and 80: QuantaMAX Filters for fish and M-FI
Page 81 and 82: FISH and M-FISH Imaging Application
Page 83 and 84: middle and bottom left images. Bott
Page 85 and 86: FLOW CYTOMETRY FILTERS Omega Optica
Page 87 and 88: FLOW CYTOMETRY - DICHROIC FILTERS D
Page 89 and 90: Optimizing Filter Sets for FRET App
Page 91 and 92: For multi-color high discrimination
Page 93 and 94: For imaging all UV-excited Qdot con
Page 95 and 96: Excitation and emission filters: 18
Page 99 and 100: Optimize Your System with the Right
Page 101 and 102: At the focal point, a fluorophore a
Page 103 and 104: choosing the optimal filter set Ple
Page 105 and 106: FLUOROPHORE REFERENCE CHART Fluorop
Page 107 and 108: Fluorophores (M-S) Fluorophores (S-
Page 109 and 110: Light Source and detector reference
Page 111 and 112: I R O P Q Intensity Crosstalk: Int
Page 113 and 114: Cleaning Optical Interference Filte
Page 115 and 116: general information Specifications
show all

optical interference filters - SPOT Imaging Solutions

Create successful ePaper yourself

Delete template?

Save as template?