optical interference filters - SPOT Imaging Solutions

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standard Filters for fish and M-FISH For maximizing multi-color labeling applications Steep edges and narrow bandwidth FISH and M-FISH Filters Arranged by fluorophores and emission wavelength. Fluorophores Filter Set SKU Components Type Product SKU Description DAPI, AMCA, Cascade Blue® XF06 Excitation XF1005 365WB50 SpectrumBlue® Dichroic XF2001 400DCLP Emission XF3002 450AF65 SpectrumAqua®, CFP, DEAC XF201 Excitation XF1201 436AF8 Dichroic XF2034 455DRLP Emission XF3075 480AF30 SpectrumGreen®, FITC, EGFP, Cy2®, Alexa Fluor® 488, Oregon Green® 488, Rhodamine GreenTM SpectrumGold®, Alexa Fluor® 532 YFP Cy3®, TRITC, Alexa Fluor® 546 5-TAMRA, BODIPY® TMR/X SpectrumOrange® XF202 Excitation XF1202 485AF20 Dichroic XF2010 505DRLP Emission XF3017 530DF30 XF203 Excitation XF1203 520AF18 Dichroic XF2203 545DRLP Emission XF3302 565DF20 XF204 Excitation XF1204 546AF10 Dichroic XF2062 555DRLP Emission XF3022 580DF30 Cy3.5® XF206 Excitation XF1206 572AF15 Dichroic XF2019 590DRLP Emission XF3020 620DF35 SpectrumRed®, Texas Red® Alexa Fluor® 568, BODIPY® TR/X Alexa Fluor® 594 Cy5®, BODIPY® 650/665-X Alexa Fluor® 647 XF207 Excitation XF1207 580AF20 Dichroic XF2020 600DRLP Emission XF3028 630DF30 XF208 Excitation XF1208 640AF20 Dichroic XF2035 650DRLP Emission XF3031 682DF22 80 For current product listings, specifications, and pricing: www.omegafilters.com • sales@omegafilters.com 1.866.488.1064 (toll free within USA only) • +1.802.254.2690 (outside USA)
FISH and M-FISH Imaging Application Note Interference Filters and Fluorescence Imaging In an upright microscope, the fluorescence illuminator follows an epi-fluorescent path (illumination from above) to the specimen. In the pathway is housed the filter blocks containing the dichroic mirror, excitation, and emission filters, which work to greatly improve the brightness and contrast of the imaged specimens, even when multiple fluorochromes are being used. Figure 1 illustrates the basic setup of the fluorescence illuminator on an upright microscope. Overview The application of in situ hybridization (ISH) has advanced from short lived, non-specific isotopic methods, to very specific, long lived, and multi-color Fluorescent-ISH probe assays (FISH). Improvements in the optics, interference filter technology, microscopes, cameras, and data handling by software have allowed for a cost effective FISH setup to be within reach of most researchers. The application of mFISH (multiplex-FISH), coupled to the advances in digital imaging microscopy, have vastly improved the capabilities for non-isotopic detection and analysis of multiple nucleic acid sequences in chromosomes and genes. Figure 2 Figure 1 XF424 SpectrumRed ® or TexasRed ® filter set XF1424 Excitation 580QM30 XF2029 Dichroic 595DRLP XF3418 Emission 63QM36 The principle components in the episcopic (reflected illumination) pathway consist of the light source (here depicted as a Mercury arc lamp), a series of lenses that serve to focus the light and correct for optical aberrations as the beam travels towards the filters, diaphragms which act to establish proper and even illumination of the specimen, and the filter turret which houses the filter sets. In the diagram it can be seen schematically how the broad band excitation light from the light source is selectively filtered to transmit only the green component by the excitation filter in the turret, which is in turn reflected by the dichroic mirror to the specimen. The red fluorescence emission is then transmitted back through the objective lens, through the mirror and is further filtered by the emission filter before visualization by eye or camera. An expanded view of the filter cube is shown in Figure 2. The excitation filter is shown in yellow and the emission filter in red to describe a typical bandpass Texas Red filter set. For current product listings, specifications, and pricing: www.omegafilters.com • sales@omegafilters.com 1.866.488.1064 (toll free within USA only) • +1.802.254.2690 (outside USA) 81
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optical interference filters For Li
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table of contents Laser Line Filter
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about us Collaboration In any inst
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Capabilities Design • Thin Film D
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intellectual property 3 RD Millenni
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photonics teaching kit Involved in
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Longpass & Shortpass Filters 515ALP
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Lot to Lot Reproducibility With the
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ColorMAX series is intended for spe
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Organic Semiconductor Overview Orga
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Filter design All boundaries betwee
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With Optical Monitoring, the intens
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highest spectral performance. Resea
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exposure to light, particularly sho
Page 29 and 30: Types of Anti-Reflective Treatments
Page 31 and 32: Figure 4 Multi-layer broadband anti
Page 33 and 34: Filter Design Considerations and Yo
Page 35 and 36: Optical Interference Filters for Ap
Page 37 and 38: Shack-Hartmann Method “Shack-Hart
Page 39 and 40: Stock and Standard products For a q
Page 41 and 42: Stock and Standard products 510BP3
Page 43 and 44: Stock and Standard products 850WB25
Page 45 and 46: Astronomy filters Throughout our hi
Page 47 and 48: Astronomy filters Projects Omega Op
Page 49 and 50: Our line of standard interference f
Page 53 and 54: For instrument developers: High vol
Page 55 and 56: Improved signal-to-noise Excellent
Page 57 and 58: Centered on the laser resonance Cl
Page 61 and 62: Full Width Half Max (FWHM) or bandw
Page 63 and 64: Steep slopes Specified by the crit
Page 65 and 66: UV capabilities We currently offer
Page 67 and 68: Dichroic Beamsplitters by Cut-On Pr
Page 69 and 70: QuantaMAX and Standard Filters for
Page 71 and 72: Summary Given the vast number of fl
Page 77 and 78: Steep edges Exceptional transmissi
Page 79: QuantaMAX Filters for fish and M-FI
Page 83 and 84: middle and bottom left images. Bott
Page 85 and 86: FLOW CYTOMETRY FILTERS Omega Optica
Page 87 and 88: FLOW CYTOMETRY - DICHROIC FILTERS D
Page 89 and 90: Optimizing Filter Sets for FRET App
Page 91 and 92: For multi-color high discrimination
Page 93 and 94: For imaging all UV-excited Qdot con
Page 95 and 96: Excitation and emission filters: 18
Page 99 and 100: Optimize Your System with the Right
Page 101 and 102: At the focal point, a fluorophore a
Page 103 and 104: choosing the optimal filter set Ple
Page 105 and 106: FLUOROPHORE REFERENCE CHART Fluorop
Page 107 and 108: Fluorophores (M-S) Fluorophores (S-
Page 109 and 110: Light Source and detector reference
Page 111 and 112: I R O P Q Intensity Crosstalk: Int
Page 113 and 114: Cleaning Optical Interference Filte
Page 115 and 116: general information Specifications
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