Granulocyte colony-stimulating factor (G-CSF ... - ResearchGate

226 BRAIN RESEARCH 1149 (2007) 223– 231
2E, arrow). No cells were double-positive for GFP and a neural
lineage marker in the injured spinal cord in both the SCI+PBS
and SCI+G-CSF groups 24 h after injury (not shown).
To confirm G-CSF-mediated mobilization of bone marrowderived
hematopoietic stem cells, we performed immunohistochemistry
for CD34 one week after injury. In the SCI+G-CSF
group, GFP- and CD34-double-positive cells were detected
near the lesion epicenter (Figs. 2G–I, arrow).
Six weeks after injury, there were many GFP-positive round
cells in the lesioned site and cells with some processes in the
white matter (Figs. 3A, B). The number of GFP-positive cells in
the SCI+G-CSF group was significantly larger than that in the
SCI+PBS group 6 weeks after injury (449.0±28.5 in the SCI+PBS
group and 610.3±21.9 in the SCI+G-CSF group, p